Two-way detection of image features and immunolabeling of lymphoma cells with one-step microarray analysis

Abstract

Detecting the number of pathological lymphoma cells and lymphocyte subtypes in blood is helpful for clinical diagnosis and typing of lymphoma. In the current study, cell type is identified by cell morphological features and immunolabeled lymphocyte subtypes. Red blood cells and leukocytes were separated using a microfluidic cell chip based on physical blood cell parameters, and leukocytes were identified using five characteristic parameters: energy variance, entropy variance, moment of inertia variance, color mean, and cell area individually. The number of red blood cells that could come into contact with the leukocyte membrane was ≤2 based on the microfluidic injection flow rate of microfluidic chips. Anti-CD3 and anti-CD19 antibodies were used for immunofluorescence staining of T-lymphocyte and B-lymphocyte surface antigens, respectively. The results suggested that the microfluidic assay could detect lymphocyte surface antigen markers and intact leukocytes. Therefore, we report a one-step microfluidic chip for classifying hematological lymphoma cells based on the physical parameters of cells, which can simultaneously measure the overall morphology of blood cells and immunolabeling of lymphocyte surface antigens in one step, solving the current problem of detecting subtypes of hematological lymphoma cells based on multiple methods and multi-step detection.

Advances in Microfluidics for Single Red Blood Cell Analysis

“…Furthermore, the IR laser capabilites to form a bubble to displace trapped cell/particle Deformability analysis of RBCs using micro-capillaries has shown experimental observations based on the RBC dynamics to detect cellular subpopulations [171]. Other prospective studies include, RBC shape assessment [313], malaria screening [314][315][316][317][318], analysis of microcapillary occlusionsickle cell disease [319][320][321][322], sickle cell disease [323,324], detection and labeling of lymphoma cells [325], blood grouping and phenotyping [326,327], and the study of dispersive RBCs flowing through microchannels [328].…”

The Use of Upconversion Nanoparticles in Prostate Cancer Photodynamic Therapy

“…It seems that reversing the negative trend can be significantly helped by the use of precision medicine, including sensitive early-stage cancer detection, as it has promising potential in terms of lower healthcare costs and better treatment outcomes [ 4 , 5 , 6 ]. The dynamic development of advanced techniques for the immunolabeling of diseased cells [ 7 ], super-resolution imaging [ 8 ] and bionanomedicine [ 9 ] has laid a good foundation and provided powerful tools for advanced theranostics [ 10 ] and the implementation of precision medicine.…”

A Microfluidic Detection System for Bladder Cancer Tumor Cells

“…The microfluidic device proposed here can be used to directly identify different cells using different morphological features under an optical microscope, similar to the automatic urine sediment analyzer. We previously reported a method used to detect hematological tumor cells and pleural effusion tumor mass cells based on microfluidics technology [16,17]. Based on previous experiments, this study applied microfluidic chip technology to establish a system for detecting urinary tumor cells.…”