Ty1 Integrase Interacts with RNA Polymerase III-specific Subcomplexes to Promote Insertion of Ty1 Elements Upstream of Polymerase (Pol) III-transcribed Genes

Cheung, Stephanie; Ma, Lina; Chan, Patrick H. W.; Hu, Hui-Lan; Mayor, Thibault; Chen, Hung-Ta; Measday, Vivien

doi:10.1074/jbc.m115.686840

Cited by 27 publications

(66 citation statements)

References 77 publications

(88 reference statements)

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“…We provide several lines of evidence supporting a major role for AC40 in recruiting Ty1 to , 2015) and this study) was not observed in a rpc53∆2-280 mutant, which decreases Ty1 integration at the SUF16 tRNA gene (Cheung et al, 2016). This suggests that either C53 secures IN1 binding to Pol III once IN1 has been recruited by AC40 or helps Ty1 integration at a step downstream of IN1 recruitment.…”

Section: Discussionmentioning

confidence: 49%

“…HA-IN1 occupancy was not completely suppressed in the three mutants, suggesting that the K 617 , S 621 or L 622 Ala substitutions may have a residual level of interaction with AC40 not detected by two-hybrid or coIP assay. Alternatively, additional protein-protein interactions, like those previously identified with other Pol III subunits, could contribute to the recruitment of Ty1 integration complex at Pol III transcribed-genes in the absence of the IN1-AC40 interaction (Cheung et al, 2016).…”

Section: Ac40 Recruits In1 At Pol I and Pol Iii-transcribed Genesmentioning

confidence: 87%

“…In addition to AC40, other Pol III subunits have been suggested to mediate the interaction between IN1 and Pol III (Cheung et al 2016). To determine whether IN1 remains associated with Pol III in the absence of the IN1-AC40 interaction, we immunoprecipitated Pol III (Oficjalska-Pham et al, 2006) in yeast cells expressing hemagglutinin (HA)-tagged C160, the largest Pol III subunit, and WT or mutant IN1 fused to streptavidin (IN1-strep).…”

Section: Ac40 Recruits In1 At Pol I and Pol Iii-transcribed Genesmentioning

confidence: 99%

“…The replacement of AC40 by AC40sp severely compromised Ty1 integration upstream of Pol III-transcribed genes, leading to a redistribution of Ty1 insertions in the genome. IN1 binding to other Pol III subunits was also described in vitro (Cheung et al, 2016). However, it is not clear whether these interactions participate to Ty1 integration site selection.…”

Section: Introductionmentioning

confidence: 99%

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The Ty1 integrase nuclear localization signal is necessary and sufficient for retrotransposon targeting to tRNA genes

Asif-Laidin

Conesa

Bonnet

et al. 2019

Preprint

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Integration of transposable elements into the genome is mutagenic. Mechanisms that target integration into relatively safe locations and minimize deleterious consequences for cell fitness have emerged during evolution. In budding yeast, the integration of the Ty1 LTR retrotransposon upstream of RNA polymerase III (Pol III)-transcribed genes requires the interaction between the AC40 subunit of Pol III and Ty1 integrase (IN1). Here we show that the IN1-AC40 interaction involves a short linker sequence in the bipartite nuclear localization signal (bNLS) of IN1. Mutations in this sequence do not impact the frequency of Ty1 retromobility, instead they decrease the recruitment of IN1 to Pol III-transcribed genes and the subsequent integration of Ty1 at these loci. The replacement of Ty5 retrotransposon targeting sequence by the IN1 bNLS induces Ty5 integration into Pol III-transcribed genes. Therefore, the IN1 bNLS is both necessary and sufficient to confer integration site specificity on Ty1 and Ty5 retrotransposons.

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Section: Discussionmentioning

confidence: 49%

Section: Ac40 Recruits In1 At Pol I and Pol Iii-transcribed Genesmentioning

confidence: 87%

Section: Ac40 Recruits In1 At Pol I and Pol Iii-transcribed Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Ty1 integrase nuclear localization signal is necessary and sufficient for retrotransposon targeting to tRNA genes

Asif-Laidin

Conesa

Bonnet

et al. 2019

Preprint

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“…Focusing on RNAP III itself, Bridier-Nahmias et al found that its AC40 subunit interacts with and is the molecular target of the Ty1 integrase [79]. Independent studies found that the Ty1-Integrase interacts with RNAP III-specific subunits to promote integration upstream of class III genes [80]. It would appear that RNAP III subunits mediate direct functional interactions with Ty1 integrase and this is a basis of integration upstream of class III transcription complexes in S. cerevisiae .…”

Section: Long Retrotransposons Target Tfiiib-rnap III Gene Complexes mentioning

confidence: 99%

RNA Polymerase III Advances: Structural and tRNA Functional Views

Arimbasseri

Maraia

2016

Trends in Biochemical Sciences

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RNA synthesis in eukaryotes is divided among three RNA polymerases (RNAP). RNAP III transcribes hundreds of tRNA genes and fewer additional short RNA genes. We survey recent work on transcription by RNAP III including an atomic structure, mechanisms of action, interactions with chromatin and retroposons, and a conserved link between its activity and a tRNA modification that enhances mRNA decoding. Other new work suggests important mechanistic connections to oxidative stress, autoimmunity and cancer, embryonic stem cell pluripotency, and tissue-specific developmental effects. We consider that, for some of its complex functions, variation in RNAP III activity levels lead to nonuniform changes in tRNA pools that can shift the translation profiles of key codon-biased mRNAs with resultant phenotypes or disease states.

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Light and shadow on the mechanisms of integration site selection in yeast Ty retrotransposon families

Bonnet

Lesage

2021

Curr Genet

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Transposable elements are ubiquitous in genomes. Their successful expansion depends in part on their sites of integration in their host genome. In Saccharomyces cerevisiae, evolution has selected various strategies to target the five Ty LTR-retrotransposon families into gene-poor regions in a genome, where coding sequences occupy 70% of the DNA. The integration of Ty1/Ty2/Ty4 and Ty3 occurs upstream and at the transcription start site of the genes transcribed by RNA polymerase III, respectively. Ty5 has completely different integration site preferences, targeting heterochromatin regions. Here, we review the history that led to the identification of the cellular tethering factors that play a major role in anchoring Ty retrotransposons to their preferred sites. We also question the involvement of additional factors in the fine-tuning of the integration site selection, with several studies converging towards an importance of the structure and organization of the chromatin.

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Ty1 Integrase Interacts with RNA Polymerase III-specific Subcomplexes to Promote Insertion of Ty1 Elements Upstream of Polymerase (Pol) III-transcribed Genes

Cited by 27 publications

References 77 publications

The Ty1 integrase nuclear localization signal is necessary and sufficient for retrotransposon targeting to tRNA genes

The Ty1 integrase nuclear localization signal is necessary and sufficient for retrotransposon targeting to tRNA genes

RNA Polymerase III Advances: Structural and tRNA Functional Views

Light and shadow on the mechanisms of integration site selection in yeast Ty retrotransposon families

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