Type 1 and Type 2 Herpes Simplex Viruses: Serological and Biological Differences

Abstract

Forty isolates of herpes simplex virus were compared by means of cross-neutralization curves. The 11 oral isolates were serotype 1, and all 29 genital/anal isolates were serotype 2. The cytopathic effects of the two serotypes were consistently different. Passage of strains of type 1 and type 2 in mice and in rabbits yielded two variants, although the majority of the strains remained unchanged serologically and in their cytopathic effects. The two variants were derived from type 1 strains and differed from the parent strains in their cytopathic effects, each of them producing syncytia and enlarged plaques. They had, however, retained the serotypic properties and the deoxyribonucleic acid (DNA) densities of their parent strains. The Roizman syncytial/macroplaque strain of herpes simplex virus was also included in the study;

Efficiency of Plating on Chick Embryo Cells and Kinetic Neutralization of Herpesvirus hominis Strains

“…Whether this antigenic difference is of sufficient magnitude and stability to warrant "type" distinctions is a matter of present controversy (6,9). Although antigenic character does not appear to vary with EOP in this or another study (8), or with other markers (12,13), there is great diversity in the degree of relatedness between strains within groups. Hampar et al (6) have suggested that H. hominis strains should be classified into one serological group as a species by the use of 7S antibodies and that late 19S antibodies should be used for determining subtypes within that species.…”

“…The examination of these herpesvirus strains suggests, as have the results of other studies (2,9,12), that neutralization divides strains into two rather distinct groups, which also differ in biological characteristics. Whether this antigenic difference is of sufficient magnitude and stability to warrant "type" distinctions is a matter of present controversy (6,9).…”

“…Nahmias et al (11) identified eight type 1 strains among 226 genital isolates, and three of Schneweis' nine genital isolates fit the type 2 designation (9). Other investigators have not reported type 1 strains from genital sources (8,12). The weight of evidence at present indicates that type 2 virus is limited to the genital tract and has a venereal mode of transmission, but it appears that at least certain type 1 viruses may also behave like genital strains.…”

Laser‐capture microdissection: Applications in routine molecular dermatopathology

“…Two distinct variants of HSV exist. HSV‐1 is usually located in the face, while HSV‐2 is responsible for genital infections 3 . If the identification of the virus is needed, the liquid in a fresh blister is subjected to PCR or negative staining can be performed using electron microscopy 4…”

The effect of pseudorabies-virus infection on cellular deoxyribonucleic acid

“…Viru8e8. Vienna Herpes simplex viruses form one serologic group, but minor antigenic differences have resulted in a classification into two types (Plummer, Waner, Phuangsab & Goodheart, 1970): type I is associated with non-genital and type II with genital infections.…”