Type 1 Pili Enhance the Invasion of <i>Salmonella braenderup</i> and <i>Salmonella typhimurium</i> to HeLa Cells

Horiuchi, Sankichi; Inagaki, Yutaka; Okamura, Noboru; Nakaya, Rintaro; Yamamoto, Naoki

doi:10.1111/j.1348-0421.1992.tb02059.x

Cited by 33 publications

(27 citation statements)

References 17 publications

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“…Type 1 fimbriae have previously been shown to confer S. enterica serotype Typhimurium adhesion to a variety of mammalian epithelial cells such as human Hep-2, HeLa, and small intestinal and bladder epithelial cell lines (Bäumler et al, 1996c;Boddicker et al, 2002;Hancox et al, 1998;Thankavel et al, 1999), and to contribute to the bacterial invasion of some of these cells (Bäumler et al, 1997;Ernst et al, 1990;Horiuchi et al, 1992;van der Velden et al, 1998). More recently, S. enterica serotype Typhimurium colonization of the murine intestine by biofilm formation was shown to require type 1 fimbriae carrying an adhesive allelic FimH (Boddicker et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Guo

Lasaro

Sirard³

et al. 2007

Microbiology

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Section: Discussionmentioning

confidence: 99%

Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Guo

Lasaro

Sirard³

et al. 2007

Microbiology

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“…Type 1 fimbriae, on the other hand, are hairlike appendages that carry adhesins specific for mannosylated glycoproteins on eukaryotic cell surfaces (2, 25, 54, 78). They are thought to be involved in pathogenesis by facilitating binding to intestinal epithelial cells (4,24,35,40,78). As with flagella, type 1 fimbriae do not appear to play a direct role in intestinal invasion but rather are thought to contribute to intestinal colonization and persistent infections (1,19).…”

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confidence: 99%

Role of Cross Talk in Regulating the Dynamic Expression of the FlagellarSalmonellaPathogenicity Island 1 and Type 1 Fimbrial Genes

et al. 2010

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Salmonella enterica, a common food-borne pathogen, differentially regulates the expression of multiple genes during the infection cycle. These genes encode systems related to motility, adhesion, invasion, and intestinal persistence. Key among them is a type three secretion system (T3SS) encoded within Salmonella pathogenicity island 1 (SPI1). In addition to the SPI1 T3SS, other systems, including flagella and type 1 fimbriae, have been implicated in Salmonella pathogenesis. In this study, we investigated the dynamic expression of the flagellar, SPI1, and type 1 fimbrial genes. We demonstrate that these genes are expressed in a temporal hierarchy, beginning with the flagellar genes, followed by the SPI1 genes, and ending with the type 1 fimbrial genes. This hierarchy could mirror the roles of these three systems during the infection cycle. As multiple studies have shown that extensive regulatory cross talk exists between these three systems, we also tested how removing different regulatory links between them affects gene expression dynamics. These results indicate that cross talk is critical for regulating gene expression during transitional phases in the gene expression hierarchy. In addition, we identified a novel regulatory link between flagellar and type 1 fimbrial gene expression dynamics, where we found that the flagellar regulator, FliZ, represses type 1 fimbrial gene expression through the posttranscriptional regulation of FimZ. The significance of these results is that they provide the first systematic study of the effect of regulatory cross talk on the expression dynamics of flagellar, SPI1, and type 1 fimbrial genes.Salmonella enterica causes a large number of diseases ranging from self-limiting gastroenteritis to life-threatening systemic infection (21, 62). Previous studies have identified multiple factors involved in Salmonella pathogenesis, including those related to motility, adhesion, invasion, and intestinal persistence (12,18,39,43,45,55,66,83,(86)(87)(88). Key among them is a type 3 secretion system (T3SS) encoded within a 40-kb region of the chromosome called Salmonella pathogenicity island 1 (SPI1) (46-48, 51, 63, 76). The SPI1 T3SS functions as a molecular hypodermic needle, enabling Salmonella to inject proteins into host cells (11-13). These injected proteins both commandeer the actin cytoskeleton to facilitate the invasion of host cells and induce inflammatory diarrhea (27,29,33,52,59,92).In addition to the SPI1 T3SS, other systems, including flagella and type 1 fimbriae, have been implicated in Salmonella pathogenesis (31,36,75). Briefly, flagella are long helical filaments attached to rotary motors embedded within the membrane that enable the bacterium to swim in liquids and swarm over surfaces (9). Flagella are thought to facilitate invasion by enabling Salmonella to swim to sites of invasion (39,75). In addition to motility, flagellin activates the expression of proinflammatory cytokines (26,60,61,77,84). Type 1 fimbriae, on the other hand, are hairlike appendages that carry adhesins...

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“…In Salmonella, these appendages have been implicated in initiating intestinal colonization, and they may contribute to tissue tropism by adhering to specific mannosylated host proteins (3,20,37,51). In addition, type 1 fimbriae of Salmonella are known to mediate binding to a number of human epithelial cell lines in vitro (4,19,29,32,51). The phenotypic expression of type 1 fimbriae is phase variable, allowing a transition between fimbriate and nonfimbriate phenotypes (1,17,21).…”

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confidence: 99%

Characterization of FimY as a Coactivator of Type 1 Fimbrial Expression in Salmonella enterica Serovar Typhimurium

Tinker

Clegg

2000

Infect Immun

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Type 1 fimbriae of Salmonella enterica serovar Typhimurium are surface appendages that carry adhesins specific for mannosylated host glycoconjugates. Regulation of the major fimbrial subunit is thought to be controlled by a number of ancillary fim genes, including fimZ, fimY, fimW, and fimU. Previous studies using a FimZ mutant have indicated that this protein is necessary for fimA expression, and in vitro DNA binding assays determined that FimZ is a transcriptional activator that binds directly to the fimA promoter. To determine the role of FimY as a potential regulator of fimbrial expression, a fimY mutant of serovar Typhimurium was generated by allelic exchange. This mutant was found to be phenotypically nonfimbriate. No transcription from the fimA promoter was detected in a fimY mutant containing a fimA-lacZ reporter construct located on the chromosome. In addition, transcription from the cloned fimY promoter was not detected in Escherichia coli unless both FimZ and FimY were present, indicating that these proteins also act as coactivators of fimY expression. Consistent with these results, there is no transcription from a fimY-lacZ reporter construct within a serovar Typhimurium fimY or fimZ mutant. Studies using the fimY-lacZ construct reveal that expression of this gene varies with environmental conditions in a manner similar to fimA expression. Extensive in vitro DNA binding assays using extracts from E. coli that overexpress FimY, as well as partially purified FimY, were unable to identify a specific interaction between FimY and the fimA or fimY promoter. The results indicate that FimY is a positive regulator of fimbrial expression and that this protein acts in cooperation with FimZ to regulate the expression of Salmonella type 1 fimbrial appendages.

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Type 1 Pili Enhance the Invasion of Salmonella braenderup and Salmonella typhimurium to HeLa Cells

Cited by 33 publications

References 17 publications

Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Role of Cross Talk in Regulating the Dynamic Expression of the FlagellarSalmonellaPathogenicity Island 1 and Type 1 Fimbrial Genes

Characterization of FimY as a Coactivator of Type 1 Fimbrial Expression in Salmonella enterica Serovar Typhimurium

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