Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells

Miyoshi, Isao; Kubonishi, Ichiro; S, Yoshimoto; Akagi, Tadaatsu; Ohtsuki, Yuji; Shiraishi, Yukimasa; Nagata, Kazuhiro; Hinuma, Yorio

doi:10.1038/294770a0

Cited by 1,345 publications

(571 citation statements)

References 3 publications

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“…Similar results have been reported by investigators in Japan, who also isolated retroviruses from A TLL cell lines [25,54]. These retroviruses are now known to be isolates of HLTV-I [52].…”

Section: B Htl V -I and Adult T Cell Leukemia/ Lymphomasupporting

confidence: 78%

The Human T-Cell Leukemia Virus Family, Adult T Cell Leukemia, and AIDS

Gallo¹,

Sarngadharan²,

Popovič³

et al. 1985

Modern Trends in Human Leukemia VI New Results in Clinical and Biological Research Including Pediatric Oncology

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“…Similar results have been reported by investigators in Japan, who also isolated retroviruses from A TLL cell lines [25,54]. These retroviruses are now known to be isolates of HLTV-I [52].…”

Section: B Htl V -I and Adult T Cell Leukemia/ Lymphomasupporting

confidence: 78%

The Human T-Cell Leukemia Virus Family, Adult T Cell Leukemia, and AIDS

Gallo¹,

Sarngadharan²,

Popovič³

et al. 1985

Modern Trends in Human Leukemia VI New Results in Clinical and Biological Research Including Pediatric Oncology

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“…The clonal origin of HUT-102, however, is unclear. MT-2, 21 MT-4, 22 C5/MJ, 23 and SLB-1 24 cell lines are HTLV-I-transformed T-cell lines, established by an in vitro coculture protocol. MT-2, MT-4 and C5/MJ are umbilical cord blood T-cell lines, whereas SLB-1 is an adult peripheral blood T-cell line.…”

Section: Cell Linesmentioning

confidence: 99%

Constitutive expression of the cyclooxygenase-2 gene in T-cell lines infected with human T cell leukemia virus type I

Mori

Inoue

Yoshida³

et al. 2001

Int. J. Cancer

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Cyclooxygenase-2 (COX-2), an inducible enzyme that catalyzes the formation of prostaglandins and other eicosanoids from arachidonic acid, is constitutively expressed in several human carcinomas. COX-2 expression, however, has not been extensively studied in leukemia. Human T cell leukemia virus type I (HTLV-I) is the etiologic agent of adult T cell leukemia, an aggressive form of human T cell malignancy. We studied COX-2 mRNA expression in various human Tcell lines. Northern blot analysis revealed that COX-2 mRNA steady-state levels were high in 4 of 7 T-cell lines infected with HTLV-I. COX-2 mRNA, however, was not expressed in any of 3 HTLV-I-negative T-cell lines. We also confirmed COX-2 expression in 6 of 7 HTLV-I-positive T-cell lines by reverse transcription-PCR. HTLV-I Tax is known to increase the expression of cellular genes and thus we assayed Tax for its ability to increase transcription from the COX-2 promoter. Although Tax increased transcription of the COX-2 promoter in a T-cell line, Tax expression did not induce COX-2 mRNA expression, indicating that Tax alone is not sufficient for significant accumulation of COX-2 mRNA, which probably requires an additional viral protein. To evaluate the potential role of COX-2 in T cells, the HTLV-Iinfected T-cell lines were treated with NS398, a selective COX-2 inhibitor. NS398 treatment inhibited proliferation and induced apoptosis of HTLV-I-infected T-cell lines and downregulated Bcl-2 and Bcl-x L mRNA expression, followed by chromosomal DNA fragmentation. Our data suggest that COX-2 is expressed selectively in T-cell lines infected with HTLV-I and that this gene may play a role in cell survival.

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“…HTLV-I infection disrupts the physiological mechanisms of T-cell growth regulation (Markham et al, 1983;Miyoshi et al, 1981;Popovic et al, 1983;Franchini, 1995). While in the early phase of transformation HTLV-I-infected T-cells require IL-2 to proliferate, in the late phase of HTLV-I transformation, T-cells appear to lose the R point control as indicated by their continuous proliferative state even in the absence of IL-2.…”

Section: Introductionmentioning

confidence: 99%