Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles

Ramírez, Orlando; Álvarez, Simón; Contreras-Kallens, Pamina; Barrera, Nelson P.; Aguayo, Sebastian; Schuh, Christian

doi:10.1080/10717544.2020.1818880

Cited by 29 publications

(33 citation statements)

References 51 publications

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“…Recent reports from our group have demonstrated the presence of EVs in Apis mellifera bee-derived products such as royal jelly and honey. 8,12 In the present study, EVs from a monofloral source of honey such as Eucryphia cordifolia (Ec) were isolated employing a similar ultracentrifugation-based approach (HEc-EVs). These vesicles were found to be mostly in the size range of <150nm, with a mode of 138nm (Figure 1B), consistent with previous reports of exosome-like EVs derived from bee products.…”

Section: Honey-derived Extracellular Vesicles Contain Antibacterial Cargo and Specific Exosomal Markersmentioning

confidence: 99%

“…8 This promising antibacterial effect was also observed for bee-derived EVs released from type-I collagen matrixes, further displaying their potential as therapeutic agents for biofilm control. 12 Despite demonstrating the presence of EVs in bee-derived products, and their effect on human and bacterial cells, questions remain regarding their specific cargo as well as the mechanisms behind their observed antibacterial and antibiofilm effect. Furthermore, their potential effect on other Gram-positive biofilm-forming bacteria such as S. mutans and S. sanguinis has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci

Leiva-Sabadini¹,

Álvarez²,

Barrera³

et al. 2021

IJN

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Purpose: Recently, our group found exosome-like extracellular vesicles (EVs) in Apis mellifera honey displaying strong antibacterial effects; however, the underlying mechanism is still not understood. Thus, the aim of this investigation was to characterize the molecular and nanomechanical properties of A. mellifera honey-derived EVs in order to elucidate the mechanisms behind their antibacterial effect, as well as to determine differential antibiofilm properties against relevant oral streptococci. Methods: A. mellifera honey-derived EVs (HEc-EVs) isolated via ultracentrifugation were characterized with Western Blot and ELISA to determine the presence of specific exosomal markers and antibacterial cargo, and atomic force microscopy (AFM) was utilized to explore their ultrastructural and nanomechanical properties via non-destructive immobilization onto poly-L-lysine substrates. Furthermore, the effect of HEc-EVs on growth and biofilm inhibition of S. mutans was explored with microplate assays and compared to S. sanguinis. AFM was utilized to describe ultrastructural and nanomechanical alterations such as cell wall elasticity changes following HEc-EV exposure. Results: Molecular characterization of HEc-EVs identified for the first time important conserved exosome markers such as CD63 and syntenin, and the antibacterial molecules MRJP1, defensin-1 and jellein-3 were found as intravesicular cargo. Nanomechanical characterization revealed that honey-derived EVs were mostly <150nm, with elastic modulus values in the low MPa range, comparable to EVs from other biological sources. Furthermore, incubating oral streptococci with EVs confirmed their antibacterial and antibiofilm capacities, displaying an increased effect on S. mutans compared to S. sanguinis. AFM nanocharacterization showed topographical and nanomechanical alterations consistent with membrane damage on S. mutans. Conclusion: Honey is a promising new source of highly active EVs with exosomal origin, containing a number of antibacterial peptides as cargo molecules. Furthermore, the differential effect of HEC-EVs on S. mutans and S. sanguinis may serve as a novel biofilmmodulating strategy in dental caries.

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Section: Honey-derived Extracellular Vesicles Contain Antibacterial Cargo and Specific Exosomal Markersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci

Leiva-Sabadini¹,

Álvarez²,

Barrera³

et al. 2021

IJN

Self Cite

View full text Add to dashboard Cite

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“…The concurrent use of royal jelly with hydroxyapatite for bone healing has been reported; however, relevant literature is lacking in the combined use of royal jelly with biomaterials [ 76 ]. Very recently, extracellular vesicles of royal jelly were incorporated in a type I collagen matrix [ 72 ]. The released vesicles modulated the capacity of fibroblasts to migrate in vitro and were effective against S. aureus biofilm formation, thus demonstrating a potential delivery system for wound healing therapies.…”

Section: Scaffold Design and Experimental Studiesmentioning

confidence: 99%

The Potential of Honeybee Products for Biomaterial Applications

Rossi

Marrazzo

2021

Biomimetics

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The development of biomaterials required continuous improvements in their properties for new tissue engineering applications. Implants based on biocompatible materials and biomaterial-based dressings are susceptible to infection threat; moreover, target tissues can suffer injuring inflammation. The inclusion of nature-derived bioactive compounds usually offers a suitable strategy to expand or increase the functional properties of biomaterial scaffolds and can even promote tissue healing. Honey is traditionally known for its healing property and is a mixture of phytochemicals that have a proven reputation as antimicrobial, anti-inflammatory, and antioxidant agents. This review discusses on the potential of honey and other honeybee products for biomaterial improvements. Our study illustrates the available and most recent literature reporting the use of these natural products combined with different polymeric scaffolds, to provide original insights in wound healing and other tissue regenerative approaches.

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“…Furthermore, the research group that first identified bee product-derived extracellular vesicles tried to develop novel type I collagen hydrogels as a continuous delivery matrix for royal jelly-derived extracellular vesicles [ 93 ]. Type I collagen hydrogels are well-established biomaterials for wound healing that can be also a local delivery system for EVs [ 107 , 108 ].…”

Section: Extracellular Vesicles As New Nutraceuticals From Bee Productsmentioning

confidence: 99%

“…Therefore, the combination of EVs and type I collagen may offer a number of benefits in wound healing therapies. Ramírez et al (2020) showed that the collagen concentration determines release patterns of royal jelly-derived extracellular vesicles and that gels containing 2 mg/mL collagen display the most stable release kinetics [ 93 ]. Released extracellular vesicles were biologically active as the integration into collagen did not alter their size or integrity.…”

Section: Extracellular Vesicles As New Nutraceuticals From Bee Productsmentioning

confidence: 99%

Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

Peršurić

Pavelić

2021

Molecules

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In recent years, interest has surged among researchers to determine compounds from bee products such as honey, royal jelly, propolis and bee pollen, which are beneficial to human health. Mass spectrometry techniques have shown that bee products contain a number of proven health-promoting compounds but also revealed rather high diversity in the chemical composition of bee products depending on several factors, such as for example botanical sources and geographical origin. In the present paper, we present recent scientific advances in the field of major bioactive compounds from bee products and corresponding regenerative properties. We also discuss extracellular vesicles from bee products as a potential novel bioactive nutraceutical component. Extracellular vesicles are cell-derived membranous structures that show promising potential in various therapeutic areas. It has been extensively reported that the use of vesicles, which are naturally formed in plant and animal cells, as delivery agents have many advantages. Whether the use of extracellular vesicles from bee products represents a new solution for wound healing remains still to be elucidated. However, promising results in specific applications of the bee products in wound healing and tissue regenerative properties of extracellular vesicles provide a good rationale to further explore this idea.

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Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles

Cited by 29 publications

References 51 publications

Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci

Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci

The Potential of Honeybee Products for Biomaterial Applications

Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

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