Type I collagen structure modulates the behavior of osteoblast-like cells

Tsai, Shih Chang; Chang, Yung; Liu, Hsuan‐Liang; Tsai, Wei‐Bor

doi:10.1016/j.jtice.2009.10.002

Cited by 20 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tsai studied the effects of three types of collagen structures (acid soluble, fibrillar and heat-denatured) on osteoblast-like cell behavior. They found that heat-denatured collagen promoted the osteoblasts to enter the differentiation stage [35] . Taubenberger [36] found that partially-denatured collagen exposes RGD-motifs and stimulates osteoblast adhesion, spreading, motility and differentiation.…”

Section: Discussionmentioning

confidence: 99%

MG63 Osteoblast-Like Cells Exhibit Different Behavior when Grown on Electrospun Collagen Matrix versus Electrospun Gelatin Matrix

et al. 2012

Self Cite

View full text Add to dashboard Cite

Electrospinning is a simple and efficient method of fabricating a non-woven polymeric nanofiber matrix. However, using fluorinated alcohols as a solvent for the electrospinning of proteins often results in protein denaturation. TEM and circular dichroism analysis indicated a massive loss of triple-helical collagen from an electrospun collagen (EC) matrix, and the random coils were similar to those found in gelatin. Nevertheless, from mechanical testing we found the Young's modulus and ultimate tensile stresses of EC matrices were significantly higher than electrospun gelatin (EG) matrices because matrix stiffness can affect many cell behaviors such as cell adhesion, proliferation and differentiation. We hypothesize that the difference of matrix stiffness between EC and EG will affect intracellular signaling through the mechano-transducers Rho kinase (ROCK) and focal adhesion kinase (FAK) and subsequently regulates the osteogenic phenotype of MG63 osteoblast-like cells. From the results, we found there was no significant difference between the EC and EG matrices with respect to either cell attachment or proliferation rate. However, the gene expression levels of OPN, type I collagen, ALP, and OCN were significantly higher in MG63 osteoblast-like cells grown on the EC than in those grown on the EG. In addition, the phosphorylation levels of Y397-FAK, ERK1/2, BSP, and OPN proteins, as well as ALP activity, were also higher on the EC than on the EG. We further inhibited ROCK activation with Y27632 during differentiation to investigate its effects on matrix-mediated osteogenic differentiation. Results showed the extent of mineralization was decreased with inhibition after induction. Moreover, there is no significant difference between EC and EG. From the results of the protein levels of phosphorylated Y397-FAK, ERK1/2, BSP and OPN, ALP activity and mineral deposition, we speculate that the mechanism that influences the osteogenic differentiation of MG63 osteoblast-like cells on EC and EG is matrix stiffness and via ROCK-FAK-ERK1/2.

show abstract

Section: Discussionmentioning

confidence: 99%

MG63 Osteoblast-Like Cells Exhibit Different Behavior when Grown on Electrospun Collagen Matrix versus Electrospun Gelatin Matrix

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Different structures composed of type I collagen have different effects on the behavior of osteoblasts. In contrast to soluble and fibrillar forms, denatured forms of type I collagen inhibit the proliferation of osteoblast-like cells and can stimulate osteoblastic differentiation (Tsai et al, 2010). A small amount of type III collagen is also found in collagen fibrils of bone.…”

Section: Regulation Of Osteoblasts By the Ecmmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

View full text Add to dashboard Cite

Bone regeneration repairs bone tissue lost due to trauma, fractures, and tumors, or absent due to congenital disorders. The extracellular matrix (ECM) is an intricate dynamic bio-environment with precisely regulated mechanical and biochemical properties. In bone, ECMs are involved in regulating cell adhesion, proliferation, and responses to growth factors, differentiation, and ultimately, the functional characteristics of the mature bone. Bone ECM can induce the production of new bone by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and the absorption of bone by osteoclasts. With the rapid development of bone regenerative medicine, the osteoinductive, osteoconductive, and osteogenic potential of ECM-based scaffolds has attracted increasing attention. ECM-based scaffolds for bone tissue engineering can be divided into two types, that is, ECM-modified biomaterial scaffold and decellularized ECM scaffold. Tissue engineering strategies that utilize the functional ECM are superior at guiding the formation of specific tissues at the implantation site. In this review, we provide an overview of the function of various types of bone ECMs in bone tissue and their regulation roles in the behaviors of osteoblast-lineage cells and osteoclasts. We also summarize the application of bone ECM in bone repair and regeneration. A better understanding of the role of bone ECM in guiding cellular behavior and tissue function is essential for its future applications in bone repair and regenerative medicine.

show abstract

“…The triple-helical conformation in Col is particularly critical for a 2 b 1 -and a 1 b 1 -dependent adhesion, and a 2 b 1 -dependent adhesion seems to involve multiple conformational binding sites, while a 1 b 1 -dependent adhesion is more restricted to the heterotrimeric native form of the molecule 2,4 .Yamamoto et al 5 identified that rabbit arterial smooth muscle cells recognized Col and DCol via interactions with triple helix-binding receptors and a chain-binding receptors. Tsai et al 6,7 suggested that the native collagen structures modulated the behavior of human osteosarcoma cells and enhanced the expression of osteopontin (OPN), collagen I (Col I), alkaline phosphatase (ALP), and osteocalcin (OCN) compared with gelatin matrix.…”

Section: Introductionmentioning

confidence: 99%

Shape-memory collagen scaffold for enhanced cartilage regeneration: native collagen versus denatured collagen

Jiang

Liu

et al. 2018

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Taken together, these insights suggest that the better proliferation, adhesion and redifferentiation of chondrocytes in Col scaffolds with the triple-helical structure may contribute to the greater cartilage repair ability. Col scaffolds may be more appropriate for repairing cartilage defects than DCol scaffolds, and DCol cannot as an alternative when using collagen-based materials for cartilage engineering applications.

show abstract

Type I collagen structure modulates the behavior of osteoblast-like cells

Cited by 20 publications

References 15 publications

MG63 Osteoblast-Like Cells Exhibit Different Behavior when Grown on Electrospun Collagen Matrix versus Electrospun Gelatin Matrix

MG63 Osteoblast-Like Cells Exhibit Different Behavior when Grown on Electrospun Collagen Matrix versus Electrospun Gelatin Matrix

The Bone Extracellular Matrix in Bone Formation and Regeneration

Shape-memory collagen scaffold for enhanced cartilage regeneration: native collagen versus denatured collagen

Contact Info

Product

Resources

About