Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid inE. coli
Weiwei Yang,
Michael Kuska,
Nan Dai
et al.
Abstract:The modified DNA base 2,6 aminopurine (2-aminoadenine, (d)Z base) was originally found in phages to counteract host encoded restriction systems. However, only a limited number of restriction endonucleases (REases) have been tested on dZ-modified DNA. Herein we report the results of 147 REases activity on dZ-modified PCR DNA. Among the enzymes tested, 53.1% are resistant or partially resistant, and 46.9% are sensitive when the restriction sites contain 1 to 6 modified bases. Sites with 4-6 dZ substitutions are … Show more
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