Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection

Mayo-Muñoz, David; Smith, Lisa F.; Garcia-Doval, Carmela; Malone, Lucia M; Harding, Kate R.; Jackson, Simon A.; Hampton, Hannah G; Fagerlund, Robert D.; Gumy, Laura F.; Fineran, Peter C.

doi:10.1016/j.molcel.2022.10.028

Cited by 38 publications

(29 citation statements)

References 82 publications

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“…Relying on these associated effectors, the long-B pAgos mediate Abi response to provide immunoprotection against invading plasmid. In particular, the long-B pAgo-nuclease system performs RNA-guided unspecific DNA degradation following recognition of a specific DNA target, indicating that both CRISPR-Cas systems and pAgo systems can utilize the target recognition-activated collateral DNA degradation as a defense strategy [30][31][32][33][34] .…”

Section: Introductionmentioning

confidence: 99%

Long-B prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Song

Lei

Liu

et al. 2023

Preprint

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Argonaute proteins (Agos) bind short nucleic acids as guides and are directed by them to recognize target complementary nucleic acids. Prokaryotic Agos (pAgos) are extremely diverse, with potential functions in microbial defense. The functions and mechanisms of a group of full-length yet inactive pAgos, long-B pAgos, remain enigmatic. Here, we show that most long-B pAgos constitute cell suicide systems together with their various associated proteins, including nucleases, Sir2-domain-containing proteins and trans-membrane proteins, respectively. Among them, the long-B pAgo-nuclease system utilizes an RNA-programmed and target-recognition-activated collateral DNA cleavage activity to sense invaders and kill the infected cells. This results in depletion of the invading plasmid from the cell population. Together, our data indicate that the long-B pAgo systems induce cell death with various effector proteins after recognition of invading nucleic acids, corresponding to an immune response via abortive infection.

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Section: Introductionmentioning

confidence: 99%

Long-B prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Song

Lei

Liu

et al. 2023

Preprint

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“…Members of the superfamily are involved in restriction-modi cation systems, DNA metabolism, tRNA splicing 40 . Recently, many members of the superfamily are found as effectors of type III CRISPR-Cas and CBASS defense systems 30,31 . We expressed EcbAgaN in E. coli BL21 and puri ed it to apparent homogeneity (Figure S2A).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA degradation are performed by some defense systems as an immune strategy [29][30][31] , and potentially by more systems that employ nuclease effectors. In particular, a few type III CRISPR-Cas systems with NucC nucleases as effectors and type V-A2 CRISPR-Cas systems cleave genomic DNA after crRNA-directed target RNA recognition 29,30 . This suggests that a subgroup of pAgo and CRISPR-Cas, the two nucleic acid-directed defense systems, have convergently evolved to adopt the genomic DNA degradation Abi strategy.…”

Section: Discussionmentioning

confidence: 99%

“…Relying on these associated effectors, the long-B pAgos mediate Abi responses to provide immunoprotection against invading plasmid. In particular, the long-B pAgo-nuclease system performs RNA-guided unspeci c DNA degradation following recognition of a speci c DNA target, indicating that both CRISPR-Cas systems and pAgo systems can utilize the target recognition-activated collateral DNA degradation as a defense strategy [29][30][31][32] . Our ndings also provide novel opportunities to develop pAgo-based technologies.…”

Section: Introductionmentioning

confidence: 99%

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Inactive long prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Song

Lei

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

Argonaute proteins (Agos) bind short nucleic acids as guides and are directed by them to recognize target complementary nucleic acids. Prokaryotic Agos (pAgos) are extremely diverse, with potential functions in microbial defense. The functions and mechanisms of a group of full-length yet inactive pAgos, long-B pAgos, remain enigmatic. Here, we show that most long-B pAgos constitute cell suicide systems together with their various associated proteins, including nucleases, Sir2-domain-containing proteins and trans-membrane proteins, respectively. Among them, the long-B pAgo-nuclease system utilizes an RNA-programmed and target-recognition-activated collateral DNA cleavage activity to sense invaders and mediate genomic DNA degradation. This kills the infected cells and results in depletion of the invader from the cell population. The data indicate that the long-B pAgo systems induce cell death with various effector proteins after recognition of invading nucleic acids, corresponding to an immune response via abortive infection.

show abstract

Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex

Tuck,

Adler,

Armbruster

et al. 2024

Cell

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Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection

Cited by 38 publications

References 82 publications

Long-B prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Long-B prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Inactive long prokaryotic Argonaute systems employ various effectors to confer immunity via abortive infection

Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex

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