Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic
            <i>Escherichia coli</i>

Bieber, David; Ramer, Sandra W.; Wu, Changjiang; Murray, William S.; Tobe, Toru; Fernandez, Rachel C.; Schoolnik, Gary K.

doi:10.1126/science.280.5372.2114

Cited by 436 publications

(437 citation statements)

References 24 publications

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“…Actin condensation beneath the attached bacteria, which indicates intimate attachment, was often detectable at this stage (data not shown). EPEC grown in DMEM produces BFP, and forms autoaggregates at the late logarithmic phase (Giron et al, 1991;Bieber et al, 1998). Removal of non-adherent bacteria 30 min after infection allowed us to examine the development of microcolonies after the initial stage of adherence.…”

Section: Microcolony Development After Initial Adherencementioning

confidence: 99%

“…BfpF is necessary for bacterial dispersal and the structural alteration of BFP from thin-to-thick bundles (Bieber et al, 1998;Knutton et al, 1999). Therefore, we examined a bfpF mutant EPEC strain, B171±8F ma , to determine whether the observed morphological changes in response to the cellular extract were mediated by BfpFregulated structural changes in BFP.…”

Section: Production Of Bfp In Adherent Epec On Caco-2 Cellsmentioning

confidence: 99%

“…Transcription of the bfp operon occurs during the logarithmic-growth phase, and is maximized in the tissue culture medium, or in the presence of calcium . Autoaggregation, which is caused by BFPmediated bacteria±bacteria interactions, occurs under optimal bfp operon-inducing conditions Bieber et al, 1998). The dispersal of bacteria from autoaggregates occurs with continuing bacterial growth to the late-logarithmic and stationary phases (Bieber et al, 1998).…”

Section: Production Of Bfp In Adherent Epec On Caco-2 Cellsmentioning

confidence: 99%

“…In vitro experiments with human epithelial cell lines have implicated bundle-forming pili (BFP) in the initial binding and in microcolony formation (Giron et al, 1991;Donnenberg et al, 1992;Sohel et al, 1996;Stone et al, 1996). BFP have been shown to be important in EPEC pathogenicity (Bieber et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…BFP biogenesis is directed by an operon of 14 genes Stone et al, 1996), including bfpA, which encodes the major subunit of the pilus fibre (Donnenberg et al, 1992;Sohel et al, 1993), and bfpP, which encodes a prepilin peptidase specific for BfpA (Zhang et al, 1994). In addition, the bfpF gene, which encodes a putative nucleotide-binding protein, is required for the dispersal phase of EPEC autoaggregation (Bieber et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Tobe¹,

Sasakawa

2001

Cellular Microbiology

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SummaryEnteropathogenic Escherichia coli (EPEC) adheres to epithelial cells and forms microcolonies in localized areas. Bundle-forming pili (BFP) are necessary for autoaggregation and the formation of microcolonies. In this study, we show that BFP, expressed by EPEC on epithelial cells, disappeared with the expansion of the microcolony. Bacterial dispersal and the release of BFP from the EPEC aggregates were induced by contact with host cellular membrane extract. In addition, BFP-expressing EPEC adhered directly to cell surfaces, in preference to attaching to pre-formed microcolonies on the cells. These results suggested that BFP mediate the initial attachment of EPEC through direct interaction with the host cell rather than through the recruitment of unattached bacteria to microcolonies on the cell.

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Section: Microcolony Development After Initial Adherencementioning

confidence: 99%

Section: Production Of Bfp In Adherent Epec On Caco-2 Cellsmentioning

confidence: 99%

Section: Production Of Bfp In Adherent Epec On Caco-2 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Tobe¹,

Sasakawa

2001

Cellular Microbiology

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Gastrointestinal Tract: Bacterial Infections

Conlon

2010

Encyclopedia of Life Sciences

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Almost every human will experience, at some stage in their life, an episode of diarrhoea due to a bacterial infection of the gastrointestinal tract. There are a variety of protective mechanisms in place, such as the high acid content of the stomach, to reduce the risk of such infections, but bacteria have evolved a variety of virulence factors, such as adhesion molecules and toxins, that enable active infections to take place. Enterotoxins, such as the cholera toxin, can cause a net secretion of water into the small bowel lumen, overwhelming the absorptive capacity of the large bowel. Cytotoxins can cause damage to the large bowel mucosa, leading to marked inflammation and causing bloody diarrhoea. Most cases of gastroenteritis can be treated with rehydration alone, but in some circumstances specific antimicrobial therapy is needed. This article highlights the pathogens implicated, the symptoms that usually result and the way such infections are managed. Key Concepts: Gastrointestinal infections due to bacteria are very common. Bacteria that cause infection of the gastrointestinal tract have developed a variety of virulence factors, including adhesion molecules and toxin secretion. Although most bacterial toxins are produced in the gut after infection, some are ingested preformed in food. Watery diarrhoea is usually the result of enterotoxins acting in the small bowel, causing net secretion of water and electrolytes into the lumen. Dysentery is bloody diarrhoea caused by bacteria that have caused inflammation in the large bowel mucosa. Although Campylobacter species are the most common cause of sporadic bacterial food poisoning, more outbreaks are caused by Salmonella species. Clostridium difficile has, in the past decade, become an important healthcare‐associated infection in hospitals in developed countries. Enterotoxigenic E. coli (ETEC) is a very common cause of travellers’ diarrhoea. Most cases of diarrhoea due to bacteria can be treated with rehydration therapy alone. Although some bacterial infections of the gastrointestinal tract require specific antibiotic therapy, empiric therapy should only be given in certain circumstances to vulnerable patients to minimise overuse of antimicrobials.

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