Type specific persistence of high risk human papillomavirus (HPV) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up study

Kjær, Susanne K.; Brule, Adriaan J. C. van den; Paull, Gerson; Svare, Edith I.; Sherman, Mark E.; Thomsen, Birthe Lykke; Suntum, Mette; Bock, Johannes E.; Poll, P; Meijer, Chris J.L.M.

doi:10.1136/bmj.325.7364.572

Cited by 382 publications

(314 citation statements)

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“…Indeed, in the work of Kjaer et al (2002) on women o29 years, PCR testing for the detection of HPV was performed on specimens collected into phosphate-buffered saline, and the authors focused their study on women with normal smears and HPV infection. In a related study, the work of Sherman et al (2003) was conducted with HC-II assay on cervicovaginal lavage specimens, a technique that is not as good for sampling the endocervical canal as direct brushing.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, in the cohort of 2432 women testing negative for HR-HPV infection followed on a period of 60 months, only two women developed an HSIL (Bory et al, 2002). Thus the NPV of a negative test with HC-II could be useful in primary screening when associated with a smear within normal limits.At the present time, there are few longitudinal studies with a detailed and prolonged follow-up of women with a normal smear with a negative HR-HPV testing (Kjaer et al, 2002;Sherman et al, 2003). Thus, the aim of the present study is to evaluate on a large population of women with an initial smear within normal limits the usefulness and limitations of a negative HR-HPV testing in such women.…”

mentioning

confidence: 98%

“…At the present time, there are few longitudinal studies with a detailed and prolonged follow-up of women with a normal smear with a negative HR-HPV testing (Kjaer et al, 2002;Sherman et al, 2003). Thus, the aim of the present study is to evaluate on a large population of women with an initial smear within normal limits the usefulness and limitations of a negative HR-HPV testing in such women.…”

mentioning

confidence: 98%

“…Moreover, only low-grade SIL (LSIL) containing HR-HPV progress to high-grade SIL (HSIL) (Koutsky et al, 1992). Owing to this, there is an increasing interest in using HPV DNA detection either alone or in addition to classic cytological examination for primary cervical screening (Cuzick et al, 1995(Cuzick et al, , 1999(Cuzick et al, , 2003Meijer et al, 1998;Clavel et al, 1999Clavel et al, , 2001Kuhn et al, 2000;Ratnam et al, 2000;Schiffman et al, 2000;Schneider et al 2000;Kjaer et al, 2002;Petry et al, 2003;Lorincz and Richart, 2003;Sherman et al, 2003). Most authors consider that a positive HPV testing selects a population at high risk for developing an HSIL, while a negative HPV testing has a very good negative predictive value (NPV).…”

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Negative human papillomavirus testing in normal smears selects a population at low risk for developing high-grade cervical lesions

et al. 2004

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High-risk human papillomaviruses (HR-HPV) are the necessary cause of cervical carcinomas and there is an increasing interest in using HR-HPV DNA detection in adjunction to cytological examination for primary cervical screening. To determine whether women with a normal smear negative for HR-HPV DNA detection with the Hybrid Capture II assay might represent a low-risk population for developing a high-grade squamous intraepithelial lesion (HSIL), 4401 women have been followed in a period of 12 -72 months (median ¼ 34 months). During this follow-up, four HSIL and one microinvasive carcinoma have been detected in this cohort (three in the cohort of 3526 women 429 years). The global negative predictive value (NPV) of double-negative tests is thus of 99.9% (ninetyfive percent confidence interval (95% CI): 99.8 -100%), whereas cytology alone gives an NPV of 99.2% (95% CI: 98.9 -99.5%). If we obtain a second negative HR-HPV test 1 -2 years after the initial test, the NPV is 100%. The NPV is also of 100% in the cohort of women 449 years. We conclude that all these women could be safely screened at longer intervals between 3 and 5 years. This policy will offset the increased costs induced by an additional HR-HPV testing in primary screening. It is now well established that oncogenic (high-risk) human papillomaviruses (HPV) are a necessary causal factor in the development of cervical intraepithelial and invasive neoplasias (Lorincz et al, 1992; Bosch et al, 1995;Walboomers et al, 1999;Zur Hausen, 2002). Infections with high-risk HPV (HR-HPV) are associated with a relative risk of between 8 and 11 for the development of squamous intraepithelial lesions (SIL) (Gaarenstroom et al, 1994). Moreover, only low-grade SIL (LSIL) containing HR-HPV progress to high-grade SIL (HSIL) (Koutsky et al, 1992). Owing to this, there is an increasing interest in using HPV DNA detection either alone or in addition to classic cytological examination for primary cervical screening (Cuzick et al, 1995(Cuzick et al, , 1999(Cuzick et al, , 2003Meijer et al, 1998;Clavel et al, 1999Clavel et al, , 2001Kuhn et al, 2000;Ratnam et al, 2000;Schiffman et al, 2000;Schneider et al 2000;Kjaer et al, 2002;Petry et al, 2003;Lorincz and Richart, 2003;Sherman et al, 2003). Most authors consider that a positive HPV testing selects a population at high risk for developing an HSIL, while a negative HPV testing has a very good negative predictive value (NPV). Indeed, considering that the mean time from detectable LSIL to preclinical invasive cancer is 12 -13 years (Gustafson and Adami, 1989), Meijer et al (1998) have proposed that women with cytologically normal smears and a negative HR-HPV test could be rescreened every 8 years.Hybrid Capture-II (HC-II), a commercial HPV detection test, (Digene, Gaithersburg, MD, USA) was introduced 7 years ago (Lorincz, 1996). Hybrid Capture-II is a nonradioactive, reproducible, relatively rapid, liquid hybridisation assay in microtiters designed to detect 18 HPV types divided into high-risk (types 16, 18, 31, 33, 35, 39, 45, 51, ...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Negative human papillomavirus testing in normal smears selects a population at low risk for developing high-grade cervical lesions

et al. 2004

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“…Carcinogenic HPV infections are extremely common and most are transient, lasting only 1-2 years at most. A number of large population cohorts of HPV and cervical neoplasia have been conducted (Bratti et al 2004;Franco et al 1999;Kjaer et al 2002), providing much information on causality and HPV natural history. These studies have provided strong empirical support for 4-stage model of cervical carcinogenesis that replaces the older model of stepwise progression through increasing severe grades of cervical intraepithelial neoplasia: 1) HPV acquisition; 2) HPV persistence [vs. clearance]; 3) neoplastic progression to cervical precancer; and 4) invasion (Wright, Jr. and Schiffman 2003).…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

LaMere

Kornegay

Fetterman

et al. 2007

Journal of Virological Methods

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Human papillomavirus (HPV) genotyping could be clinically useful, depending on the results of large, prospective studies like the HPV Persistence and Progression cohort. The cohort is based on genotyping and follow-up of Hybrid Capture-positive women at Kaiser Permanente, Northern California. HPV DNA testing by Hybrid Capture 2 requires denaturation with alkali, possibly damaging the DNA for optimal PCR-based genotyping. A feasibility study was conducted on paired aliquots of anonymized specimens from 100 women with low-grade intraepithelial lesion cytology. Test aliquots were left in denaturant for 10 or 18 hours at 4°C and then neutralized; comparison aliquots were not denatured but diluted to match the timing, temperature, concentration and salt conditions of the treated specimens. The masked aliquots were tested using a commercialized PCRbased assay that detects of 37 HPV genotypes. There was no overall effect of treatment on test Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. positivity or number of types. HPV16 was marginally more likely to be detected in untreated versus treated aliquots (P = 0.09) but HPV45 was marginally more likely to be detected in treated than untreated aliquots (P = 0.07), suggesting that these differences represented chance (intra-test variability). It can be concluded that residual Hybrid Capture-positive specimens can be accurately genotyped by PCR after Hybrid Capture 2 processing. NIH Public Access

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Current trends in the etiology and diagnosis of HPV‐related head and neck cancers

Chai

Lambie²,

Verma

et al. 2015

Cancer Medicine

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Human papilloma virus (HPV) infection is a major risk factor for a distinct subset of head and neck squamous cell carcinoma (HNSCC). The current review summarizes the epidemiology of HNSCC and the disease burden, the infectious cycle of HPV, the roles of viral oncoproteins, E6 and E7, and the downstream cellular events that lead to malignant transformation. Current techniques for the clinical diagnosis of HPV-associated HNSCC will also be discussed, that is, the detection of HPV DNA, RNA, and the HPV surrogate marker, p16 in tumor tissues, as well as HPV-specific antibodies in serum. Such methods do not allow for the early detection of HPV-associated HNSCC and most cases are at an advanced stage upon diagnosis. Novel noninvasive approaches using oral fluid, a clinically relevant biological fluid, allow for the detection of HPV and cellular alterations in infected cells, which may aid in the early detection and HPV-typing of HNSCC tumors. Noninvasive diagnostic methods will enable early detection and intervention, leading to a significant reduction in mortality and morbidity associated with HNSCC.

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Type specific persistence of high risk human papillomavirus (HPV) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up study

Cited by 382 publications

References 10 publications

Negative human papillomavirus testing in normal smears selects a population at low risk for developing high-grade cervical lesions

Negative human papillomavirus testing in normal smears selects a population at low risk for developing high-grade cervical lesions

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

Current trends in the etiology and diagnosis of HPV‐related head and neck cancers

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