Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells

Abstract

a b s t r a c tIn search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display-and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis.

Single-Cell RNA Sequencing of Bone Marrow Mesenchymal Stem Cells from the Elderly People

“…COL11A1 plays an essential role during the cartilage development process ( 32 ). Type V collagen might be considered as a stromal component that impairs osteogenesis ( 33 ). It can be seen that many top 20 DEGs in cluster 2 are related to multidirectional differentiation of BMSCs.…”

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

“…It was shown that the differentiation of stem cells toward osteoblasts depends on numerous signaling pathways and that the process itself could be enhanced and regulated by certain growth factors [8,[238][239][240][241][242]. Some growth factors are already approved by the FDA as drugs for treatment, such as the recombinant human bone morphogenic protein 7 (rhBMP7) in 2001 or the rhBMP2 in 2002 [243,244].…”

Engineering the Tenogenic Niche In Vitro with Microenvironmental Tools

“…[96] It has been used along with various tenogenic inductive treatments, [97][98][99][100] as it facilitates enhanced collagen type I synthesis in vitro. [101,102] Low glucose dose has also been shown [102] Glucose Low doses upregulated expression of scleraxis, SOX9, COL1A1, and COL1A2, but not COL2A [103] TSPCs Tendon-derived matrix Stimulated proliferation and maintained the stemness [216] Collagen type I/fibronectin Demonstrated greater attachment on fibronectin than on collagen [217] Exhibited spindle-shaped morphology with long processes on collagen, but not on fibronectin [217] BMSC Collagen type I Tenogenic differentiation was improved when PDMS replicas of Achilles tendon sections were coated with collagen type I, however this was shown to be concentration-dependent [218] Collagen type I/fibronectin Collagen promoted scleraxis, tenomodulin and tenascin C expression, and inhibited osteogenic differentiation, while fibronectin upregulated RUNX-2 and ALP [91] ADSCs Tendon-derived matrix/fibronectin Deposited more collagen type I and exhibited greater collagen type I to collagen type III ratio on tendon-derived matrix [219] Tendon-derived matrix hydrogel/Collagen type I hydrogel Tendon-derived matrix demonstrated higher proliferation; upregulation of scleraxis, tenascin C, and tenomodulin; enhanced mechanical properties; lower osteogenic potential [220] Collagen type V Reduction of osteogenic differentiation markers, even in the presence of osteogenic media [221] Gelatin Did not show any additional benefits beyond improved adhesion [222] Did not affect proliferation and decreased protein and collagen synthesis [223] 2.7 Tenocyte proliferation was increased at early passage cells [207,212] TSPCs 0.5 Inhibited self-renewal capacity and reduced adipogenic, chondrogenic, and osteogenic potential in differentiation media [224] 2 Increased proliferation, retained their tenogenic differentiation potential, and had a lower osteogenic, chondrogenic, and adipogenic differentiation capacity [212] Reduced Sox-9 and RUNX-2 expression level, did not affect PPAR expression and culture in differentiation media led to enhanced adipogenesis, chondrogenesis, and osteogenesis [225] 5…”