Types and Designs of Bioreactors for Hairy Root Culture

Choi, Yoon E.; Kim, Yoon-Soo; Paek, Kee-Yoeup

doi:10.1007/1-4020-3694-9_9

Cited by 10 publications

(13 citation statements)

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“…A) is one of the most popular industrial scale bioreactor systems. This design includes stirrers that facilitate mass transfer and regulate medium currency . Typical stirrers are propellers and pinched‐blade turbines that cause axial fluid motion.…”

Section: Bioreactors Designs For Cultivation Of Differentiated Plant mentioning

confidence: 99%

“…Mean power input per volume was suggested to be kept under 1000 W m 3 . The controllability of the parameters of cultivation (pH, temperature, dissolved oxygen, and nutrient concentration) can be performed in a better way than with any other type of bioreactor design . Although it is often employed for high‐density cultures, this type of bioreactor was originally not considered suitable for differentiated plant in vitro cultures cultivation due to several limitations: high shear stress; high power consumption; problems with inoculation and harvesting biomass after the end of a cultivation due to the propeller are possible .…”

Section: Bioreactors Designs For Cultivation Of Differentiated Plant mentioning

confidence: 99%

“…The cultivation of plant in vitro systems is being considered as a prospective opportunity for production of valuable phytochemicals . In this way, numerous restrictions are being avoided including ecological problems, work with restricted or endangered species or genus, seasonal, and environmental factors and offering the advantages of cultivation under controlled conditions (safer production and stick rigidity to good manufacturing practice; reduced contamination; simpler downstream processes; shorter production cycles and constancy of the quality of the products ) in any place or season . Secondary metabolites are by definition a product of differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Bioprocessing of differentiated plant in vitro systems

Steingroewer

Bley

Georgiev

et al. 2012

Engineering in Life Sciences

123

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Plant cells contain a wide range of interesting secondary metabolites, which are used as natural pigments and flavoring agents in foods and cosmetics as well as phyto‐pharmaceutical products. However, conventional industrial extraction from whole plants or parts of them is limited due to environmental and geographical issues. The production of secondary metabolites from in vitro cultures can be considered as alternative to classical technologies and allows a year‐round cultivation in the bioreactor under optimal conditions with constant high‐level quality and quantity. Compared to plant cell suspensions, differentiated plant in vitro systems offer the advantage that they are genetically stable. Moreover, the separation of the biomass from culture medium after fermentation is much easier. Nevertheless, several investigations in the literature described that differentiated plant in vitro systems are instable concerning the yield of the target metabolites, especially in submerged cultivations. Other major problems are associated with the challenges of cultivation conditions and bioreactor design as well as upscaling of the process. This article reviews bioreactor designs for cultivation of differentiated plant in vitro systems, secondary metabolite production in different bioreactor systems as well as aspects of process control, management, and modeling and gives perspectives for future cultivation methods.

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Section: Bioreactors Designs For Cultivation Of Differentiated Plant mentioning

confidence: 99%

Section: Bioreactors Designs For Cultivation Of Differentiated Plant mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioprocessing of differentiated plant in vitro systems

Steingroewer

Bley

Georgiev

et al. 2012

Engineering in Life Sciences

123

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show abstract

“…Due to their intrinsic configuration, they are more suitable for research-oriented applications, although mass micropropagation of many species is also feasible, provided an appropriate protocol exists. Larger systems (up to 20 000 l or more) [13,23] are specifically designed, home-built models, which are operated to fulfill particular requirements and, therefore, are not widely available. It should be remembered that as scale-up of the culture increases, considerable compromises to shear stress, mixing and oxygen transfer are required, the calculation of which lies beyond the scope of this chapter.…”

Section: Medium Scale Disposable or Semidisposable Airlift Reactorsmentioning

confidence: 99%

“…They are also taller and thinner and have no moving parts. This type of bioreactor is of the bubble column or the airlift design [1, 2, 12,13].…”

Section: Introductionmentioning

confidence: 99%

Bioreactors

and

Anthony

2010

Plant Cell Culture

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Culture Establishment, Plant Cell

Eibl

Rischer

et al. 2009

Encyclopedia of Industrial Biotechnology

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Alternatively to whole plants, plant cell cultures are used to produce bioactive substances for food industry, cosmetics and pharmacy. This mainly concerns secondary metabolites and recombinant proteins (so‐called plant made proteins, PMPs). Among the employed culture types (which comprise suspension cultures, root cultures and shoot cultures), plant cell suspensions induced via callus cultures dominate. This fact can be explained by the suspension culture's morphology being less complex than those of root and shoot cultures, which allows easier cell banking and cultivation up to m 3 ‐scale. Based on a summary of semi‐ and commercial plant cell‐derived products the preferable culture types and their establishment are described in more detail. Finally, most common indirect and direct methods of gene transformation ( Agrobacterium ‐and virus‐mediated transformation, particle bombardment, polyethylene glycol method, electroporation, microinjection) and of cell banking (slow growth storage, cryopreservation) are discussed for highly productive cell lines of plant origin.

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Types and Designs of Bioreactors for Hairy Root Culture

Cited by 10 publications

References 0 publications

Bioprocessing of differentiated plant in vitro systems

Bioprocessing of differentiated plant in vitro systems

Bioreactors

Culture Establishment, Plant Cell

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