Types of cell death and methods of their detection in yeast <i>Saccharomyces cerevisiae</i>

Wloch‐Salamon, Dominika; Bem, A.E.

doi:10.1111/jam.12024

Cited by 37 publications

(25 citation statements)

References 77 publications

(153 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result confirms captan non-specific thiol reactivity as one of the most important direct effects of the phtalimide fungicides [3]. Moreover, captan-treated cells exhibited a drastic loss of membrane integrity, a typical necrotic behavior [16], which can be the result of the modification of membrane protein structures by protein sulfhydryldisulfide transitions, and consequent increase in membrane permeability [17]. As previously observed in another thiol-reactant fungicide Mancozeb [18], captan-treated cells exhibited an important increase in ROS concentration.…”

Section: Resultssupporting

confidence: 74%

The effect of the fungicide captan onSaccharomyces cerevisiaeand wine fermentation

et al. 2016

View full text Add to dashboard Cite

Abstract. Fungicides, particularly those used during grape maturation, as captan, can affect the natural yeast population of grapes, and can reach grape must affecting wine fermentation. The objective of the present work was to study the effect of captan on the viability and fermentative behavior of S. cerevisiae. S. cerevisiae (BY4741) on exponential phase was treated with captan (0 to 40 µM) for different periods, and their cell viability analyzed. Cell membrane integrity, thiols concentration, and reactive oxygen species (ROS) accumulation was determined. The fermentation experiments were conducted in synthetic must using wine yeast strain Y904. The results showed that under aerobic conditions, 20 µM of captan reduce 90% of yeast viability in 6 hours. Captan treated cells exhibited alteration of membrane integrity, reduction of thiol compounds and increase in intracellular ROS concentration, suggesting a necrotic and pro-oxidant activity of the fungicide. Fermentative experiments showed that concentrations above 2.5 µM captan completely inhibited fermentation, while a dose dependent fermentation delay associated with the reduction of yeast viability was detected in sub-inhibitory concentrations. Petit mutants increase was also observed. In conclusion, the captan induces yeast necrotic cell death on both aerobic and anaerobic conditions causing fermentation delay and/or sucking fermentations.

show abstract

Section: Resultssupporting

confidence: 74%

The effect of the fungicide captan onSaccharomyces cerevisiaeand wine fermentation

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In response to stresses, S. cerevisiae could regulate different biological processes in the cell [6]–[8], [41], [42], involving phosphatidylserine, mitochondrial membrane, ROS and nucleus [3], [43]. For example, pheromone stress can induce phosphatidylserine externalization and cause the hyperpolarization of mitochondrial membrane (the elevation of ΔΨ m ), the ROS production, and then the fragmentation of mitochondria (the decrease of ΔΨ m ) and the release of cytochrome c to finally induce cell death [41].…”

Section: Discussionmentioning

confidence: 99%

Response of Saccharomyces cerevisiae to the Stimulation of Lipopolysaccharide

Shen

Jiang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Lipopolysaccharide, known as endotoxin, can stimulate potent host immune responses through the complex of Toll-like-receptor 4 and myeloid differentiation protein 2; but its influence on Saccharomyces cerevisiae, a model organism for studying eukaryotes, is not clear. In this study, we found that lipopolysaccharide-treated S. cerevisiae cells could be stained by methylene blue, but did not die. Transcriptional profiling of the lipopolysaccharide-treated S. cerevisiae cells showed that 5745 genes were modulated: 2491 genes up-regulated and 3254 genes down-regulated. Significantly regulated genes (460 up-regulated genes and 135 down-regulated genes) in lipopolysaccharide-treated S. cerevisiae cells were analyzed on Gene Ontology, and used to establish physical protein-protein interaction network and protein phosphorylation network. Based on these analyses, most of the regulated genes in lipopolysaccharide-treated S. cerevisiae cells were related to cell wall, membrane, peroxisome and mitochondrion. Further experiments demonstrated that lipopolysaccharide stimulation caused the exposure of phosphatidylserine and the increase of mitochondrial membrane potential in S. cerevisiae cells, but levels of intracellular reactive oxygen species and metacaspase activation were not increased. This study demonstrated that lipopolysaccharide stimulation causes significant changes in S. cerevisiae cells, and the results would contribute to understand the response of eukaryotic cells to lipopolysaccharide stimulation.

show abstract

“…PCD in yeast displays many of the same cellular landmarks as apoptosis in higher organisms, such as DNA fragmentation, cell surface changes, and involvement of mitochondria and reactive oxygen species (ROS) 9495. For this reason, yeast PCD is often referred to as "yeast apoptosis".…”

Section: Varying Fates Varying Functionsmentioning

confidence: 99%

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation

Honigberg¹

2016

Microbial Cell

View full text Add to dashboard Cite

Diploid budding yeast (Saccharomyces cerevisiae) can adopt one of several alternative differentiation fates in response to nutrient limitation, and each of these fates provides distinct biological functions. When different strain backgrounds are taken into account, these various fates occur in response to similar environmental cues, are regulated by the same signal transduction pathways, and share many of the same master regulators. I propose that the relationships between fate choice, environmental cues and signaling pathways are not Boolean, but involve graded levels of signals, pathway activation and master-regulator activity. In the absence of large differences between environmental cues, small differences in the concentration of cues may be reinforced by cell-to-cell signals. These signals are particularly essential for fate determination within communities, such as colonies and biofilms, where fate choice varies dramatically from one region of the community to another. The lack of Boolean relationships between cues, signaling pathways, master regulators and cell fates may allow yeast communities to respond appropriately to the wide range of environments they encounter in nature.

show abstract

Types of cell death and methods of their detection in yeast Saccharomyces cerevisiae

Cited by 37 publications

References 77 publications

The effect of the fungicide captan onSaccharomyces cerevisiaeand wine fermentation

The effect of the fungicide captan onSaccharomyces cerevisiaeand wine fermentation

Response of Saccharomyces cerevisiae to the Stimulation of Lipopolysaccharide

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation

Contact Info

Product

Resources

About