TYPES OF HEMOLYSINS PRODUCED BY
            <i>STAPHYLOCOCCUS AUREUS</i>
            , AS DETERMINED BY THE REPLICA PLATING TECHNIQUE

Haque, Riaz-Ul; Baldwin, Jack N.

doi:10.1128/jb.88.5.1442-1447.1964

Cited by 19 publications

(6 citation statements)

References 11 publications

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“…Fibrinolytic activity was noted on medium containing 12% human citrated plasma (18). The determination of the production of a-, ,B-, and 8-hemolysins was with rabbit, sheep, and human erythrocytes, which had been added to heart infusion agar to a final concentration of 5%, by the replica plating technique (9). Pigment formation was observed on agar (Difco) supplemented with 3% skim milk powder (4).…”

Section: Methodsmentioning

confidence: 99%

Animal fecal carriership and biotypes of Staphylococcus aureus

Dimitracopoulos¹,

Kalkani-Boussiakou²,

Papavassiliou³

1977

Appl Environ Microbiol

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Staphylococcus aureus was found in 44% of the rectal swabs from 377 pigs and in 12% of the swabs from 147 cows. Seventy-one percent of the isolates from pigs showed fibrinolytic activity and grew in colonies of the negative violet type on crystal violet agar. In this group, 47% of the isolates coagulated both human and bovine plasmas and were characterized as intermediate type, whereas 24% coagulated human plasna and were classified as human biotype A. Among the fibrinolysin-negative isolates, 15, 12, and 2% were identifiable as animal biotypes B, C, and E, respectively. The cow isolates were classified as intermediate type (51%), human biotype A (40%o), and animal biotype C (9%).

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Section: Methodsmentioning

confidence: 99%

Animal fecal carriership and biotypes of Staphylococcus aureus

Dimitracopoulos¹,

Kalkani-Boussiakou²,

Papavassiliou³

1977

Appl Environ Microbiol

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“…This medium prevented the emergence of variants producing lysins other than ,B-hemolysin. A representative number of colonies were frequently checked for the production of 3-hemolysin by the replica plating tech-nique described by Haque and Baldwin (1964), with the use of sheep, rabbit, human, and horse blood agar plates.…”

Section: Methodsmentioning

confidence: 99%

Purification and Properties of Staphylococcal Beta-Hemolysin I

Haque

Baldwin

1964

J Bacteriol

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HAQUE, RIAZ-UL (Ohio State University, Columbus), AND JACK N. BALDWIN. Purification and properties of staphylococcal beta-hemolysin. I. Production of beta-hemolysin. J. Bacteriol. 88:1304-1309. 1964.-Highest activity of f-hemolysin was observed when buffered saline (pH 7.0) containing 0.001 M magnesium sulfate was used as a diluent, and the tubes were incubated at 37 C for 80 min and then refrigerated for 30 min. Either Heart Infusion semisolid agar or a dialysate of Heart Infusion containing 0.3% agar was suitable for the production of large quantities of ,B-hemolysin. The concentration of ,B-hemolysin in semisolid and broth cultures was greatest after incubation for 24 hr. Continued incubation resulted in a loss of active hemolysin in broth cultures but not in semisolid agar cultures. Incubation in atmospheres containing 20% carbon dioxide greatly enhanced the production of fl-hemolysin. The presence of fermentable sugars inhibited the production of ,3-hemolysin. Highest yields of fl-hemolysin were obtained when the initial pH of the medium was 5.5 to 5.8. 3-Hemolysin is one of a variety of diffusible substances produced by Staphylococcus aureus. The only property of 3-hemolysin which is universally accepted is the "hot-cold" lysis of sheep or bovine red blood cells. Other properties of the culture filtrates of S. aureus were also ascribed to /-hemolysin. Thus, Bryce and Rountree (1936) found that f-hemolysin was toxic for guinea I Taken in part from a thesis submitted by R. Haque to the Graduate School of The Ohio State University in partial fulfillment of the requirements for M.S. degree.

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“…Production of a-, /3-, and 8-hemolysins was noted on rabbit, sheep, and human blood agar plates, respectively (7).…”

Section: Subcommittee On Taxonomy Of Staphylococci Andmentioning

confidence: 99%

Staphylococci from the feces of different animal species: biotypes of Staphylococcus aureus strains of sheep and goat origin

Dimitracopoulos¹,

Sakellariou²,

Papavassiliou³

1976

Appl Environ Microbiol

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Staphylococcus aureus was found in 96% of the rectal swabs from 133 sheep and in 80% of the swabs from 125 goats. Seventy-seven percent of the isolates from both hosts exhibited the fibrinolytic and growth characteristics of human biotype A on human plasma and crystal violet agar, repsectively, but 99% of these isolates resembled S. aureus of animal origin in their other properties. Only 21% of the sheep and 24% of the goat isolates were clearly identifiable as human biotype A and animal biotypes B and C.

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TYPES OF HEMOLYSINS PRODUCED BY STAPHYLOCOCCUS AUREUS , AS DETERMINED BY THE REPLICA PLATING TECHNIQUE

Abstract: , AND JACK N. BALDWIN. Types of hemolysins produced by Staphylococcus aureus as determined by the replica plating technique.

Cited by 19 publications

References 11 publications

Animal fecal carriership and biotypes of Staphylococcus aureus

Animal fecal carriership and biotypes of Staphylococcus aureus

Purification and Properties of Staphylococcal Beta-Hemolysin I

Staphylococci from the feces of different animal species: biotypes of Staphylococcus aureus strains of sheep and goat origin

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