Types of Microbicidal and Microbistatic Agents

Al-Adham, Ibrahim; Haddadin, Randa N.; Collier, Phillip J.

doi:10.1002/9781118425831.ch2

Cited by 22 publications

(28 citation statements)

References 444 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are synthesized from food-grade organic acids and do not generate any toxic by-products. Like PAA, the three other peroxyacids appear to be bactericidal and fungicidal and to destroy spores (Al-Adham et al 2013). These non-specific oxidizers would leave few possibilities for the development of resistance in microorganisms.…”

Section: Discussionmentioning

confidence: 98%

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

2014

View full text Add to dashboard Cite

This study was conducted to evaluate the efficacy of four different peroxyacids, namely peracetic (PAA), perpropionic (PPA), perlactic (PLA), and percitric (PCA) for inactivating viruses in suspension or attached to stainless steel or polyvinyl chloride surfaces. The test virus was a proxy for human norovirus, namely murine norovirus 1. Plaque-forming units in suspension (10(7) per mL) were treated with 50-1,000 mg L(-1) peroxyacid (equilibrium mixture of organic acid, hydrogen peroxide, peroxyacid, and water) for 1-10 min. Inactivation was measured by plaque assay. PAA and PPA were the most effective, with a 5 min treatment at 50 mg L(-1) being sufficient to reduce viral titer by at least 3.0 log10, whether the virus was in suspension or attached to stainless steel or polyvinyl chloride disks under clean or fouled conditions. Combinations of organic acid and hydrogen peroxide were found ineffective. Similar inactivation was observed in the case of virus in artificial biofilm (alginate gel). These short super-oxidizers could be used for safe inactivation of human noroviruses in water or on hard surfaces.

show abstract

Section: Discussionmentioning

confidence: 98%

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

2014

View full text Add to dashboard Cite

show abstract

“…The healthy, unbroken and pink nodules were separated from roots by using sterile razor blade and placed in petri plates. These nodules were surface-disinfected with 95% ethanol for twenty seconds followed by immersing in 0.2% mercuric chloride (HgCl 2 ) solution for three minutes and five times washing with sterile-distilled water [35]. After that, nodules were aseptically crushed with a glass rod in a test tube containing distilled water, and a final suspension of 1 mL was prepared.…”

Section: Isolation Of Endophytic Strainsmentioning

confidence: 99%

“…Seven P-solubilizing endophytic strains were evaluated for their ability to promote growth of chickpea under natural climatic conditions. The YEMB media was cultured with overnight grown bacterial strains and incubated at 28 ± 1 • C for 48 h. Seeds of chickpea were surface-disinfected by dipping in 95% ethanol for twenty seconds then 0.2% HgCl 2 solution for three minutes and rinsed five times with sterilized distilled water [35]. Three surface-disinfected seeds were inoculated with each of selected bacterial culture by dipping in the respective broth for 30 min.…”

Section: Screening Of Endophytes For Growth Promotion Of Chickpea (Jamentioning

confidence: 99%

Appraising Endophyte–Plant Symbiosis for Improved Growth, Nodulation, Nitrogen Fixation and Abiotic Stress Tolerance: An Experimental Investigation with Chickpea (Cicer arietinum L.)

et al. 2019

View full text Add to dashboard Cite

Chickpea is an important leguminous crop that improves soil fertility through atmospheric nitrogen fixation with the help of rhizobia present in nodules. Non-rhizobia endophytes are also capable of inducing nodulation and nitrogen fixation in leguminous crops. The aim of the current study was to isolate, characterize and identify the non-rhizobia endophytic bacterial strains from root nodules of chickpea. For this purpose, more than one hundred isolates were isolated from chickpea root nodules under aseptic conditions and were confirmed as endophytes through re-isolating them from root nodules of chickpea after their inoculation. Nineteen confirmed endophytic bacterial strains revealed significant production of indole acetic acid (IAA) both in presence and absence of L-tryptophan and showed their ability to grow under salt, pH and heavy metal stresses. These strains were evaluated for in vitro plant growth promoting (PGP) traits and results revealed that seven strains showed solubilization of P and colloidal chitin along with possessing catalase, oxidase, urease and chitinase activities. Seven P-solubilizing strains were further evaluated in a jar trial to explore their potential for promoting plant growth and induction of nodulation in chickpea roots. Two endophytic strains identified as Paenibacillus polymyxa ANM59 and Paenibacillus sp. ANM76 through partial sequencing of the 16S rRNA gene showed the maximum potential during in vitro PGP activities and improved plant growth and nodulation in chickpea under the jar trial. Use of these endophytic strains as a potential biofertilizer can help to reduce the dependence on chemical fertilizers while improving crop growth and soil health simultaneously.

show abstract

“…Oxidizing agents in particular are used for spore killing, in particular in applied settings, where agents such as hydrogen peroxide (H 2 O 2 ) and sodium hypochlorite are used (Al‐Adham et al . ). The mechanism whereby these latter two agents kill spores has been reasonably well studied, and despite the potential genotoxicity of these agents, they do not kill spores by DNA damage (Young and Setlow ; Cortezzo et al .…”

Section: Introductionmentioning

confidence: 97%

Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite

Setlow

et al. 2013

Letters in Applied Microbiology

View full text Add to dashboard Cite

Significance and Impact of the Study: This work shows that with Bacillus subtilis spore populations in which approximately 95% of individual spores were killed by several oxidizing agents, >95% of the spores in these populations germinated with nutrients, albeit slowly. This is important, as assay of an early germination event, release of dipicolinic acid, has been suggested as a rapid assay for spore viability and would give false-positive readings for the level of the killing of oxidizing agent-treated spore populations. Analysis of the germination kinetics of multiple individual untreated or oxidizing agenttreated spores also provides new information on proteins damaged by oxidizing agent treatment, and at least some of which are in spores' inner membrane. AbstractMore than 95% of individuals in populations of Bacillus subtilis spores killed approximately 95% by hydrogen peroxide or hypochlorite germinated with a nutrient, although the germination of the treated spores was slower than that of untreated spores. The slow germination of individual oxidizing agent-treated spores was due to: (i) 3-to 5-fold longer lag times (T lag ) between germinant addition and initiation of fast release of spores' large dipicolinic acid (DPA) depot (ii) 2-to 10-fold longer times (DT release ) for rapid DPA release, once this process had been initiated; and (iii) 3-to 7-fold longer times needed for lysis of spores' peptidoglycan cortex. These results indicate that effects of oxidizing agent treatment on subsequent spore germination are on: (i) nutrient germinant receptors in spores' inner membrane (ii) components of the DPA release process, possibly SpoVA proteins also in spores' inner membrane, or the cortex-lytic enzyme CwlJ; and (iii) the cortex-lytic enzyme SleB, also largely in spores' inner membrane. This study further indicates that rapid assays of spore viability based on measurement of DPA release in spore germination can give false-positive readings.

show abstract

Types of Microbicidal and Microbistatic Agents

Cited by 22 publications

References 444 publications

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

Study of the Virucidal Potential of Organic Peroxyacids Against Norovirus on Food-Contact Surfaces

Appraising Endophyte–Plant Symbiosis for Improved Growth, Nodulation, Nitrogen Fixation and Abiotic Stress Tolerance: An Experimental Investigation with Chickpea (Cicer arietinum L.)

Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite

Contact Info

Product

Resources

About