Typhonium flagelliforme induces apoptosis in CEMss cells via activation of caspase-9, PARP cleavage and cytochrome c release: Its activation coupled with G0/G1 phase cell cycle arrest

Mohan, Syam; Abdul, Ahmad Bustamam; Abdelwahab, Siddig Ibrahim; Al-Zubairi, Adel Sharaf; Sukari, Mohamed Aspollah; Abdullah, Rasedee; Taha, Manal Mohamed Elhassan; Ibrahim, Mohamed Yousif; Syam, Suvitha

doi:10.1016/j.jep.2010.07.043

Cited by 57 publications

(48 citation statements)

References 45 publications

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“…A recent study reported that MCPH1 is involved in E2F1-mediated apoptosis; knockdown of MCPH1 in HEK293 cells resulted in decreased expression of E2F1 target genes, such as caspase-3, caspase-7, BRCA1 and Chk1 (23), which are important in cell apoptosis. Mitochondria participate in the intrinsic apoptotic pathway and tumors arise more frequently through the intrinsic pathway than the extrinsic pathway as a result of the sensitivity of the intrinsic pathway to apoptosis (24). In the intrinsic pathway, apoptosis is mediated by proteins in the Bcl-2 family.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Zhou

Bai

et al. 2015

Oncology Letters

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Abstract. Microcephalin (MCPH1/BRIT1) is a large nuclear protein that is involved in the early cellular response to DNA damage, the expression of which is reduced in a variety of types of human tumors. A recent study by our group demonstrated that MCPH1 expression is markedly decreased in lung cancer. However, it remains unclear whether inducing the expression of MCPH1 may ameliorate lung cancer, and, if so, which mechanisms underlie this process. The results of the present study demonstrated that MCPH1 expression was downregulated in lung cancer tissues compared with that in normal lung tissues. Furthermore, MCPH1 overexpression in A549 non-small cell lung carcinoma cells, successfully inhibited cell proliferation via arrest of the cell cycle in the S and G2/M phases. In addition, MCPH1 overexpression promoted cell apoptosis, in association with a significant increase in the quantities of Bax and active caspase-3, as well as a decrease in the level of Bcl-2. In conclusion the present results indicated that MCPH1 is involved in the regulation of apoptosis and entry into mitosis, suggesting that MCPH1 may function as a tumor suppressor and that it may be important in the pathogenesis of lung cancer.

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Section: Discussionmentioning

confidence: 99%

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Zhou

Bai

et al. 2015

Oncology Letters

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“…Herbs studied for leukemia therapy include Hibiscus cannabinus [3], Vernonia amygdalina root [4] Euphorbia formosana [5], Allium sativum [6], Moringa oleifera [7] and Typhonium flagelliforme [8]. Morinda citrifolia (Noni) or `mengkudu' in Malaysia has been extensively used for its broad therapeutic effects, including various anticancer activities, in both clinical practice and laboratory animal models [9].…”

Section: Introductionmentioning

confidence: 99%

“…The extract dose dependently induced leukemia cells apoptosis both in vitro and in vivo restored all pathological blood, liver and spleen changes in vivo and reinstated the endogenous immune responses to normal healthy levels in vivo. [7] and Typhonium flagelliforme [8]. Morinda citrifolia (Noni) or `mengkudu' in Malaysia has been extensively used for its broad therapeutic effects, including various anticancer activities, in both clinical practice and laboratory animal models [9].…”

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Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model

Ahmadi

et al. 2017

JABB

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Leukemia affects over a million people worldwide and disturbs the bone marrow, the spleen and the lymph system. In finding dietary therapy against leukemia the vegetable Morinda citrifolia leaves were investigated and compared with ATRA (all-trans-retinoic acid) in mice. The extract standardised to epicatechin and scopoletin was anti-proliferative against human JURKAT and murine WEHI-3B leukemia cells (IC 50 of 14.5 and 17μg /ml respectively) in vitro yet was nontoxic to normal peripheral blood mononuclear cells. Leukemia was induced in the BALB/c mice by inoculating WEHI-3B cells subcutaneously. Three days after leukemia development the extract (100 and 200mg /Kg BW) or ATRA (5mg/ kg BW) were fed daily to the mice for four weeks. The extract decreased the leukemic mice peripheral blood immature leukocytes, anemia, total white blood cells; bone marrow blasts cells content and blast cells invasions to the spleen and liver. Electron microscopy and histopathology observations confirmed the extract induced leukemia cells apoptosis similar to ATRA. The extract also mitigated the changes in T-cells and B-cells levels in the leukemic spleen tissues in vivo. The extract dose dependently induced leukemia cells apoptosis both in vitro and in vivo restored all pathological blood, liver and spleen changes in vivo and reinstated the endogenous immune responses to normal healthy levels in vivo. [7] and Typhonium flagelliforme [8]. Morinda citrifolia (Noni) or `mengkudu' in Malaysia has been extensively used for its broad therapeutic effects, including various anticancer activities, in both clinical practice and laboratory animal models [9]. The Morinda citrifolia leaves are often eaten as vegetables after blanching by the locals of South East Asia. The leaves are also traditionally used medicinally to treat wound infections, pain, arthritis, swellings and for general health. The leaves have liver-protective, antioxidant and skin healing effects [10]. The leaves are scientifically reported to have no acute, sub-acute or sub-chronic oral toxicity [11]. The oral no observed-adverseeffect level (NOAEL) of M. citrifolia leaves ethanolic extract is 1000 mg/kg [12]. This work demonstrates the therapeutic effects of the edible Morinda citrifolia (Mengkudu/Noni) leaf extract for leukemia and the possible modes of action in mice model. Keywords Materials and Methods Plant materialsM. citrifolia leaves (Voucher No. SK2322/14, identified by Biodiversity Unit, Institute of Bioscience, University Putra Malaysia, Serdang) were collected from Institute Bioscience, Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model 2/11Copyright: ©2017 Ahmadi et al. University Putra Malaysia, State of Selangor, Malaysia. The leaves were dried, coarsely ground and soaked overnight in 50% aqueous ethanol in the leaf: solvent ratio (w/v) of 1:5 (kg/l), with occasional stirring. The extract was filtered and rotary evaporated to remove all solvents. The yield was 13.6%. The leaf extract is standardized using HPLC (Waters 2996, Milford, MA)...

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“…Cleaved procaspase-8 may either directly activate caspase-3 or induce the cleavage of p22/BID, the BH-3 domain-only sub-group of BCL-2 family, to generate a truncated p15 BID (tBID). The truncated p15BID translocates to the mitochondria and promotes the release of Cyt c. 6,7 In the receptor-independent mechanism, multiple pro-apoptotic signals converge at the mitochondrial level to cause the release of Cyt c from the mitochondria. Cyt c synergistically acts with Apaf-1 to activate caspase-9, and subsequently release caspase-3.…”

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confidence: 99%

Inhibitory effects of ginseng sapogenins on the proliferation of triple negative breast cancer MDA-MB-231 cells

Kwak

Park

Lee

et al. 2014

Bioorganic & Medicinal Chemistry Letters

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Typhonium flagelliforme induces apoptosis in CEMss cells via activation of caspase-9, PARP cleavage and cytochrome c release: Its activation coupled with G0/G1 phase cell cycle arrest

Abstract: Collectively, results presented in this study demonstrated that the DCM/F7 fraction inhibited the proliferation of leukemia cells, leading to the programmed cell death, which was confirmed to be through the mitochondrial pathway.

Cited by 57 publications

References 45 publications

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model

Inhibitory effects of ginseng sapogenins on the proliferation of triple negative breast cancer MDA-MB-231 cells

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