The study’s objective was to overview all Centaureinae species recorded in Lithuania, evaluate the distribution and population state of the newly discovered four alien species and provide information on the residence status, distribution and habitats occupied by all species of the subtribe. The subtribe Centaureinae in Lithuania is now represented by five genera: Centaurea, Leuzea, Psephellus, Serratula and Zoegea. The largest genus in the flora of the country is Centaurea, which includes four native and six alien species. The genus Serratula is represented by one native species, while the genera Leuzea, Psephellus and Zoegea each have one alien species. Tree alien species (an archaeophyte, Centaurea cyanus, and neophytes Centaurea montana and Psephellus dealbatus) are naturalised aliens, whereas Centaurea cheiranthifolia, Centaurea diffusa, Centaurea iberica, Centaurea macrocephala, Leuzea repens and Zoegea crinita are casual aliens. Five species of the subtribe Centaureinae (Centaurea jacea, Centaurea phrygia, Centaurea scabiosa, Centaurea stoebe and Serratula tinctoria) are native to Lithuania. Maps of the distribution of newly discovered alien species in Lithuania are presented.