Typing for Human Alloantigens with the Primed Lymphocyte Typing Technique

Morling, Niels; Jakobsen, B. K.; Platz, P.; Ryder, L. P.; Svejgaard, A.; Thomsen, Mögens

doi:10.1016/s0065-2776(08)60721-x

Cited by 13 publications

(15 citation statements)

References 219 publications

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“…This pattern of response was not related to MLC-induced proliferation, which was depressed in 9 DS subjects tested and is in keeping with previous observations of CTL activity in cultures with negative MLC-induced proliferation [6,7,42,65]. This pattern of response was not related to MLC-induced proliferation, which was depressed in 9 DS subjects tested and is in keeping with previous observations of CTL activity in cultures with negative MLC-induced proliferation [6,7,42,65].…”

Section: Discussionsupporting

confidence: 92%

Cell-mediated cytotoxicity in down syndrome: impairment of allogeneic mixed lymphocyte reaction, NK and NK-like activities

et al. 1988

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Peripheral blood mononuclear cells (PBMC) from 16 non-institutionalized patients with Down syndrome (DS) were studied with various monoclonal antibodies and analysed for natural killer (NK), and NK-like activity. Lymphocyte proliferation and cytotoxic T-lymphocyte (CTL) cytotoxicity generated in mixed lymphocyte culture (MLC) were also evaluated in 11 DS patients. Phenotypic characterization of PBMC from DS subjects confirms our previous findings of high numbers of CD8+ lymphocytes and HNK-1+, and CD16+ cells. Lymphocyte proliferation and CTL cytotoxicity generated in MLC were low or absent in most patients. NK activity was low in almost all DS patients, while NK-like cytotoxicity generated in MLC was normal in the majority and did not correlate with NK activity from unstimulated PBMC.

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Section: Discussionsupporting

confidence: 92%

Cell-mediated cytotoxicity in down syndrome: impairment of allogeneic mixed lymphocyte reaction, NK and NK-like activities

et al. 1988

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“…Our homozygous tissue typing cells are used as stimulator cells in the mixed lymphocyte culture to define HLA-D determinants (17) and several of them have been included in various international histocompatibility workshops (8,17), which also have proven their reliability as typing reagents. Therefore, it is remarkable that digestion with BamHI resulted in patterns of restriction fragments that were unique to nearly each of the HLA-Dw/DR types defined by these cells, the only exceptions being Dw2 and Dw6.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the results with Pst I suggest that our probe detects heterozygosity within the HLA-D region. Therefore, it is possible that the homozygous typing cells are not homozygous for all HLA-D (3- (8).…”

Section: Discussionmentioning

confidence: 99%

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Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Owerbach¹,

Lernmark²,

Rask³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class H antigen j-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamfIl, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HIA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as P8t I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region. f-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.The HLA-D region class II antigens are membrane, proteins that are expressed on many cells involved in the immune response. The antigens are highly polymorphic and seem to determine a number of cellular immune functions (1, 2). The HLADw/DR determinants defined by serological and mixed lymphocyte culture test are associated not only with certain diseases, often of autoimmune character (3, 4), but also with characteristics of the immune response to antigens in normal individuals (5-7). Present evidence suggests that class II antigens are encoded 'by several loci (DR, DC, SB) on the short arm of chromosome 6 (cf. ref. 8). The polymorphic character of the class II antigens resides in the 3 chain, whereas the a chain appears less heterogeneous (9-11). Restriction enyzme polymorphism analysis of genomic DNA with cloned cDNA of class II antigen a-chain or (-chain mRNA showed single hybridization bands with a-chain (12-14) but showed multiple bands with 13-chain probes (14,15).In the present study we have used the HLA-D region (3chain cDNA probe pDR-f3-1 (11,16) Hybridization Probe. The probe for hybridization was derived from the recombinant plasmid pDR-/3-1, which contains DNA sequences complementary to a class II antigen 83-chain mRNA (11,16), the sequence of which demonstrates homology to the mouse I-A class, II antigens and is tentatively thought to represent a HLA-DC A chain (11). The pDR-/3-1 cDNA was digested with Pst I and Pvu II and two fragments representing nucleotides 1-790 of the clone (14) were isolated by agarose gel electrophoresis, followed by electroelution into dialysis tubing. These restriction fragments correspond to most of the coding region and some 3' nontranslated sequences and do not contain naturally occurring EcoRI, BamHI, or Pst I restriction sites (11,16

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“…that the blastogenic response depends on determinants in the HLA region in addition to HLA-DR (Morling et al, 1982). Already in Histocompatibility Testing 1980 several groups reported the existence of clusters of sera determining factors supertypic to HLA-DR which could be governed by genes separate from but in strong association with DR.…”

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confidence: 99%