Typing of French isolates of infectious haematopoietic necrosis virus (IHNV) with monoclonal antibodies using indirect immunofluorescence

Danton, Malcolm; Ristow, Sandra S.; Hattenberger-Baudouy, A. M.; Kinkelin, P. de

doi:10.3354/dao018223

Cited by 3 publications

(6 citation statements)

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“…The weaker reaction with AU-9695338, DE-DF 4/99-8/99, FR-32/87 and IT-217A could be due to substitutions of these amino acids. French isolates, including isolate 32/87, of IHNV have been typed previously with MAb 1NDW14D using indirect immunofluorescence (Danton et al 1994). Not including isolate 32/87, only 5 of the 27 isolates tested reacted with MAb 1NDW14D (Danton et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Section: Infectious Haematopoietic Necrosis Virus (Ihnv) Is the Causamentioning

confidence: 99%

“…Electrophoretic typing, monoclonal antibody (MAb) analysis, T1 ribonuclease fingerprinting and gene sequencing of IHNV isolates have shown that identical or almost identical isolates are found within a certain geographic location independent of host species (Hsu et al 1986, Ristow & Arnzen de Avila 1991, Oshima et al 1995. Among the European isolates, a panel of French isolates have previously been studied using MAbs against the N protein in indirect immunofluorescence (Danton et al 1994) and the IT-217A and FR-32/87 isolates have been characterised using MAbs against the G protein ( Arkush et al 1989).In the present study the complete N gene and a 605 nt partial G gene sequence of 6 representative IHNV isolates from Italy, France, Germany, Austria and Russia were determined and compared with isolates from the 3 major genogroups from the west coast of USA and Canada. Our results confirm previous work and expand our understanding of European IHNV to include isolates from additional countries, with phylogenetic trees constructed based on analysis of a different viral gene.…”

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confidence: 99%

“…French isolates, including isolate 32/87, of IHNV have been typed previously with MAb 1NDW14D using indirect immunofluorescence (Danton et al 1994). Not including isolate 32/87, only 5 of the 27 isolates tested reacted with MAb 1NDW14D (Danton et al 1994).It has been shown that IHNV isolates found within a localised geographic area typically have identical or nearly identical nucleotide sequences, independent of host species (Hsu et al 1986, Ristow & Arnzen de Avila 1991, Oshima et al 1995. In phylogenetic studies the European IHNV isolates are divided into genogroups and subgenogroups corresponding to the geographic region they were isolated from, whereas in serological studies one exception is found with the isolate DE-DF 13/98 11621 from eel (the other European isolates being isolated from rainbow trout), which reacted differently with MAb 1NDW14D.…”

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confidence: 99%

“…In phylogenetic studies the European IHNV isolates are divided into genogroups and subgenogroups corresponding to the geographic region they were isolated from, whereas in serological studies one exception is found with the isolate DE-DF 13/98 11621 from eel (the other European isolates being isolated from rainbow trout), which reacted differently with MAb 1NDW14D. Considerable variation among the epitopes of the nucleoprotein of French IHNV isolates from rainbow trout has previously been shown (Danton et al 1994). The different reaction of DE-DF 13/98 11621 can therefore be due to either the considerable variation among the epitopes in general among IHNV isolates, or to DE-DF 13/98 11621 having been isolated from a different species.…”

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Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Johansson¹,

Einer-Jensen²,

Batts³

et al. 2009

Dis. Aquat. Org.

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Infectious haematopoietic necrosis virus (IHNV) causes the lethal disease infectious haematopoietic necrosis (IHN) in juvenile salmon and trout. The nucleocapsid (N) protein gene and partial glycoprotein (G) gene (nucleotides 457 to 1061) of the European isolates IT-217A, FR-32/87, DE-DF 13/98 11621, DE-DF 4/99-8/99, AU-9695338 and RU-FR1 were sequenced and compared with IHNV isolates from the North American genogroups U, M and L. In phylogenetic studies the N gene of the Italian, French, German and Austrian isolates clustered in the M genogroup, though in a different subgroup than the isolates from the USA. Analyses of the partial G gene of these European isolates clustered them in the M genogroup close to the root while the Russian isolate clustered in the U genogroup. The European isolates together with US-WRAC and US-Col-80 were also tested in an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (MAbs) against the N protein. MAbs 136-1 and 136-3 reacted equally at all concentrations with the isolates tested, indicating that these antibodies identify a common epitope. MAb 34D3 separated the M and L genogroup isolates from the U genogroup isolate. MAb 1DW14D divided the European isolates into 2 groups. MAb 1DW14D reacted more strongly with DE-DF 13/98 11621 and RU-FR1 than with IT-217A, FR-32/87, DE-DF 4/99-8/99 and AU-9695338. In the phylogenetic studies, the Italian, French, German and Austrian isolates clustered in the M genogroup, whereas in the serological studies using MAbs, the European M genogroup isolates could not be placed in the same specific group. These results indicate that genotypic and serotypic classification do not correlate.KEY WORDS: Infectious haematopoietic necrosis virus · IHNV · Europe · Nucleocapsid protein · Glycoprotein · Phylogeny · Enzyme-linked immunosorbent assay · ELISA Resale or republication not permitted without written consent of the publisherDis Aquat Org 86: [213][214][215][216][217][218][219][220][221] 2009 phosphoprotein (P), matrix protein (M), glycoprotein (G) and the polymerase (L); and one non-virion (NV) protein (Fauquet et al. 2005).IHNV was first observed in cultured sockeye salmon on the west coast of North America (Bootland & Leong 1999). Currently IHNV in North America is found from Alaska to northern California along the Pacific coast and inland to Idaho (Kurath et al. 2003). In Asia IHNV has been isolated from fry and fingerlings of Pacific salmon Oncorhynchus spp. and rainbow trout in Japan (Nishizawa et al. 2006) and from juvenile rainbow trout and masu salmon Oncorhynchus masou in Korea (Kim et al. 2007). In 1987 IHNV was isolated from rainbow trout yolk sac fry in Italy (Bovo et al. 1987) and from rainbow trout fry and fingerlings in France (Laurencin 1987). Since then isolation of IHNV in Europe has been reported from Austria (Kolodziejek et al. 2008), Germany (Fichtner et al. 2000, Enzmann et al. 2005, Belgium (World Organisation for Animal Health 2007) and Switzerland (Knuesel et al. 2003). The first outbreak of IHN in...

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Section: Discussionmentioning

confidence: 99%