Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide

Simoons-Smit, Ina M.; Appelmelk, Ben J.; Verboom, Theo; Negrini, Riccardo; Penner, J L; Aspinall, G. O.; Moran, Anthony P.; Fei, S F; Shi, Bingtian; Rudnica, W; Savio, Antonella; Graaff, J. de

doi:10.1128/jcm.34.9.2196-2200.1996

Cited by 154 publications

(86 citation statements)

References 28 publications

(29 reference statements)

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“…Of the 57 Irish isolates, biomass was available from 37 for detection of Le antigen expression. Le x and/or Le y epitopes were detected on 89% of the H. pylori isolates which is comparable to that observed on isolates from other parts of the world [23,24]. Of potential interest, none of the Irish isolates expressed Le , Le , sialyl-Le x and H type 1 chain which have been detected on H. pylori isolates from other geographical populations [23,24,56].…”

Section: Discussionsupporting

confidence: 60%

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Marshall

1999

FEMS Immunology and Medical Microbiology

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The cagA gene, vacA gene, CagA (cytotoxin-associated gene A product) and VacA (vacuolating cytotoxin) status of a collection of Helicobacter pylori isolates from the geographically distinct Irish population was determined, the potential association of these traits with Lewis (Le) antigen expression was assessed, and the relationship between these bacterial properties and the pathology associated with H. pylori infection was evaluated. Of the 57 isolates, a higher proportion from ulcer than from non-ulcer patients expressed VacA (71% vs. 53%). H. pylori isolates which were cagA-positive were no more significantly associated with peptic ulcers than non-ulcer disease (71% vs. 67%, P = 0.775), nor were CagA-positive isolates (57% vs. 50%, P = 0.783), but 80% of the isolates from duodenal ulcer patients were cagA-positive. Thirty-seven of the 57 isolates were tested for Le antigen expression. No statistically significant relationship (P > 0.05) was found between the occurrence and level of expression of Le(x) or Le(y) and cagA, vacA, or VacA status. This lack of an association in the Irish H. pylori isolates contrasts with that previously reported for predominantly North American isolates, and may be attributable to the adaptation of H. pylori strains with differing attributes to different human populations.

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Section: Discussionsupporting

confidence: 60%

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Marshall

1999

FEMS Immunology and Medical Microbiology

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“…This is in contrast to the L-and P-selectins which require these acidic groups for binding [16,30^32]. Non-acidic sequences of Le a and Le x types have been detected both immunochemically and chemically on a considerable proportion of H. pylori isolates investigated to date [33,34]. The sialyl-Le x sequence has so far been reported on one H. pylori strain, P466 [35].…”

Section: Discussionmentioning

confidence: 99%

Interactions of the gastrotropic bacteriumHelicobacter pyloriwith the leukocyteâendothelium adhesion molecules, the selectins â a preliminary report

Galustian

Elviss²,

Chart³

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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The deleterious effects of Helicobacter pylori infection of the stomach are largely the result of a vigorous chronic inflammatory response, and include chronic gastritis, peptic ulceration and gastric cancer. We are exploring the possibility that carbohydrate components on H. pylori contribute to the persistent inflammation through interactions with leukocyte-endothelial adhesion molecules of the host. Lipopolysaccharides of most H. pylori strains contain sequences related to the Lewis (Le(x) or Le(a)) antigens. Carbohydrate sequences of this family encompass ligands for the leukocyte-endothelium adhesion molecules of the host, namely, the E- and P-selectins, which are expressed on inflamed endothelia, and L-selectin, which is constitutively expressed on leukocytes. Here we investigate H. pylori isolates from patients with chronic gastritis, duodenal ulcer and gastric cancer for their interactions with the selectins. Our results provide unequivocal evidence of interactions of isolates from each of the diagnostic groups with E- and L-selectins.

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“…Several typing methods have been described for H. pylori among which are biotyping [5], multi-locus enzyme electrophoresis [6], serotyping [7], restriction fragment length polymorphism (RFLP) of genomic DNA [8^10] or polymerase chain reaction (PCR) products [11^13], ribotyping [14], pulsed-¢eld gel electrophoresis [15], random ampli¢cation of polymorphic DNA (RAPD) [16], repetitive sequence element polymerase chain reaction (REP-PCR) [17] and DNA^DNA hybridisation [18]. The majority of these methods con¢rm the genetic heterogeneity of H. py-lori.…”

Section: Introductionmentioning

confidence: 99%

Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP-PCR and restriction fragment end-labelling

Doorn¹

1998

FEMS Microbiology Letters

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Genetic diversity of 32 Helicobacter pylori strains isolated from patients with gastritis, gastric or duodenal ulcer, carcinoma, or lymphoma was determined by repetitive sequence element polymerase chain reaction (REP-PCR), and by the new typing method restriction fragment end-labelling (RFEL). Furthermore, these two methods were used to investigate a possible correlation between clinical symptoms and the genetic background of Helicobacter pylori. Both REP-PCR and RFEL revealed 31 different patterns for the 32 strains tested, but the pair of isolates with identical REP-PCR patterns was not the same as the pair of isolates with identical RFEL patterns. Computer-assisted analysis of the DNA fingerprints was used to determine similarity coefficients. This analysis revealed no clustering of disease-specific strains by any of the two methods.

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Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide

Cited by 154 publications

References 28 publications

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Interactions of the gastrotropic bacteriumHelicobacter pyloriwith the leukocyteâendothelium adhesion molecules, the selectins â a preliminary report

Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP-PCR and restriction fragment end-labelling

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Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide

Cited by 154 publications

References 28 publications

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Lack of a relationship between Lewis antigen expression and cagA, CagA, vacA and VacA status of Irish Helicobacter pylori isolates

Interactions of the gastrotropic bacteriumHelicobacter pyloriwith the leukocyteâendothelium adhesion molecules, the selectins â a preliminary report

Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP-PCR and restriction fragment end-labelling

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Interactions of the gastrotropic bacteriumHelicobacter pyloriwith the leukocyteâendothelium adhesion molecules, the selectins â a preliminary report