Typing of Human Mycobacterium avium Isolates in Italy by IS 1245 -Based Restriction Fragment Length Polymorphism Analysis

Abstract

All but 2 of 63 Mycobacterium avium isolates from distinct geographic areas of Italy exhibited markedly polymorphic, multibanded IS1245 restriction fragment length polymorphism (RFLP) patterns; 2 isolates showed the low-number banding pattern typical of bird isolates. By computer analysis, 41 distinct IS1245 patterns and 10 clusters of essentially identical strains were detected; 40% of the 63 isolates showed genetic relatedness, suggesting the existence of a predominant AIDS-associated IS1245 RFLP pattern.

Comparison of Mycobacterium avium isolates from Greek AIDS and human immunodeficiency virus-negative patients by pulsed-field gel electrophoresis

“…More recent studies are also divided in their findings. Using IS 1245 typing, identical M. avium strains were found to infect both immunocompromised and immunocompetent patients, and to be geographically widespread in Denmark [24], while a predominantly AIDS‐associated IS 1245 RFLP pattern was recognized among Italian strains from distinct geographic areas [25]. On the other hand, using the same typing method, diverse M. avium isolates from HIV‐seropositive and ‐negative children have also been reported [26].…”

Molecular Analysis of Mycobacterium avium Isolates by Using Pulsed-Field Gel Electrophoresis and PCR

“…Different laboratory methods, including serotyping (41), multilocus enzyme electrophoresis (45), restriction fragment length polymorphism (RFLP) analysis and hybridization to specific probes (4,10,11,14,15,19,29,32,33), and pulsedfield gel electrophoresis (PFGE) (2,4,7,23,24,36), have been applied for these purposes. The last two methods mentioned are DNA-based methods and typically use agarose gel electrophoresis of restriction enzyme-digested genomic DNA which is stained directly with ethidium bromide (PFGE) or which is transferred to membranes and probed with labeled DNA (RFLP analysis).…”