Typing ofPantoea agglomeransisolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey

Loncaric, Igor; Heigl, Helmut; Licek, Elisabeth; Moosbeckhofer, Rudolf; Busse, Hans‐Jürgen; Rosengarten, Renate

doi:10.1051/apido/2008062

Cited by 49 publications

(34 citation statements)

References 43 publications

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“…The SDS-PAGE was performed as described by Loncaric et al 2009. Groups with similar protein patterns consisting of at least three isolates were subjected to analyses of respiratory quinones (representative strains only) and further genotypic characterization (enterobacterial repetitive intergenic consensus (ERIC)-PCR, ARDRA, 16S rDNA sequence analysis).…”

Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis Ofmentioning

confidence: 99%

“…Bacterial DNA extraction was as described by Loncaric et al 2009. Primers used for ERIC-PCR were as described by Versalovic et al (1991).…”

Section: Genetic Characterizationmentioning

confidence: 99%

“…Honey bees, like other social insects, harbor a wide variety of bacteria, due to the high density of individuals within the colony, the sharing of food, and the coexistence of colony members from multiple generations (Evans and Armstrong 2006;Loncaric et al 2009;Kačániová et al 2009). …”

Section: Introductionmentioning

confidence: 99%

“…The honey bees have been used as a vector for establishment of these three antagonistic of E. amylovora in apple and pear orchards (Thomson et al 1992;Loncaric et al 2008). A. pullulans and P. agglomerans are commonly found in honey bee's environment (Loncaric et al 2008(Loncaric et al , 2009). There are also other bacteria found in the honey bee environment including members of Burkholderia (Palumbo et al 2007), Acinetobacter (Evans and Armstrong 2006;Liu et al 2007), Pseudomonas (de Werra et al 2008), and Stenotrophomonas (Eva ns a nd Ar mstr on g 20 0 6; Romanenko et al 2008) that exhibit antagonistic activity against several different pathogens such as mycotoxigenic fungi or P. larvae.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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-This study was conducted to improve the knowledge about bacteria associated with honey bees, Apis mellifera carnica. In this survey, the diversity of Gram-negative non-fermenting bacteria isolated and cultivated from pollen loads, honey sac, freshly stored nectar, and honey was investigated. Bacteria were characterized by a polyphasic approach. Based on morphological and physiological characteristics and comparison of isolates protein patterns after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 11 protein similarity groups were established and confirmed by enterobacterial repetitive intergenic consensus PCR. One isolate, representing a protein similarity group (representative strain), was characterized in more detail by analysis of respiratory quinones, amplified ribosomal DNA restriction analysis, and 16S rDNA sequence analysis. Based on the results of these examinations, seven representative strains were identified as members of the genus Pseudomonas. The remaining representative strains were allocated to the genera Acinetobacter, Chryseobacterium, Stenotrophomonas, and Commamonas.bacterial diversity / PCR / honey / honey bees / 16S rDNA gene

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Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis Ofmentioning

confidence: 99%

“…Bacterial DNA extraction was as described by Loncaric et al 2009. Primers used for ERIC-PCR were as described by Versalovic et al (1991).…”

Section: Genetic Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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“…Originally a plant bacterium, it is known both as an epiphytic microbe developing on the surface of plants and as an endophytic organism living inside the plants [7,8]. The bacterium also occurs abundantly in plant and animal products, in the body of arthropods and other animals, in water, soil, dust and air, and occasionally in humans [7,9,10]. From the human viewpoint, the role of this organism is ambiguous -both deleterious and beneficial: on one side, it causes disorders in people exposed to inhalation of organic dusts [11] and diseases of crops [12], and on the other, it produces substances effective in the treatment of cancer and other diseases of humans and animals [13], suppresses the development of various plant pathogens by antibiotic production and/or competition [7,14], promotes plant growth by nitrogen fixation and other mechanisms [5,7,8], and appears as a potentially efficient bio-fertilizer and bio-remediator [7,15].…”

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confidence: 99%

Pantoea agglomerans: a marvelous bacterium of evil and good. Part I. Deleterious effects: Dust-borne endotoxins and allergens – focus on cotton dust

Dutkiewicz¹,

Maćkiewicz²,

Lemieszek³

et al. 2015

Ann Agric Environ Med.

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The ubiquitous Gram-negative bacterium Pantoea agglomerans (synonyms: Enterobacter agglomerans, Erwinia herbicola) is known both as an epiphytic microbe developing on the surface of plants, and as an endophytic organism living inside the plants. The bacterium also occurs abundantly in plant and animal products, in the body of arthropods and other animals, in water, soil, dust and air, and occasionally in humans. From the human viewpoint, the role of this organism is ambiguous -both deleterious and beneficial: on one side it causes disorders in people exposed to inhalation of organic dusts and diseases of crops, and on the other it produces substances effective in the treatment of cancer and other diseases of humans and animals, suppresses the development of various plant pathogens, promotes plant growth, and appears as a potentially efficient biofertilizer and bioremediator. P. agglomerans has been identified as a predominant bacterium on cotton plants grown worldwide, usually as an epiphyte, rarely as pathogen. It is particularly numerous on cotton bract after senescence. During the processing of cotton in mills, bacteria and their products are released with cotton dust into the air and are inhaled by workers, causing respiratory and general disorders, usually defined as byssinosis. The most adverse substance is endotoxin, a heteropolymer macromolecule present in the outermost part of the cell wall, consisting of lipopolysaccharide (LPS) as a major constituent, phospholipids and protein. Numerous experiments carried out in last quarter of 20th century on laboratory animals and human volunteers supported convincing evidence that the inhaled endotoxin produced by P. agglomerans causes numerous pathologic effects similar to those elicited by cotton dust, such as the influx of free lung cells into airways and activation of alveolar macrophages which secrete mediators (prostaglandins, platelet-activating factor, interleukin-1, tumour necrosis factor) that cause the accumulation of platelets in pulmonary capillaries, initiating acute and chronic inflammation, resulting in endothelial cell damage and extravasation of cells and fluids into the lung interstitium. These changes cause bronchoconstriction, the decrement of lung function expressed as reduction of forced expiratory volume in one second (FEV 1 ) and/or diffusion capacity, increase in the airway hyper-reactivity and subjective symptoms such as fever, airway irritation and chest tightness. The conclusions from these experiments, performed mostly 20-30 years ago, did not loose their actuality until recently. This was because to-date no other cotton dust component had been identified as a more important work-related hazard than bacterial endotoxin. Although other microbial and plant constituents are also considered as potential causative agents of byssinosis, the endotoxin produced by Pantoea agglomerans and other Gram-negative bacteria present in cotton dust, is still regarded as a major cause of this mysterious disease. Key wordsPantoea agglomerans, end...

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Linking bacterial diversity to floral identity in the bumble bee pollen basket

et al. 2020

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Typing ofPantoea agglomeransisolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey

Cited by 49 publications

References 43 publications

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

<i>Pantoea agglomerans</i>: a marvelous bacterium of evil and good. Part I. Deleterious effects: Dust-borne endotoxins and allergens – focus on cotton dust

Linking bacterial diversity to floral identity in the bumble bee pollen basket

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