Typing of <i>Staphylococcus epidermidis</i> clinical strains by a selected panel of Brazilian killer yeasts

Perez, Leandro Reus Rodrigues; d’Azevedo, Pedro Alves; Pazzini, Fabiano; Schrank, Augusto; Vainstein, Marilene Henning; Valente, Patrícia

doi:10.1002/jobm.200700143

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…Klebsiella pneumonia, Clostridium tetani, Enterococcus faecalis, Escherichia coli, Bacillus subtilis, Bacillus cereus and Citrobacter koseri. These bacteria have also been isolated from hospital SMW and reported by other authors [16][17][18][19]. Enterococcus faecalis and Enterococcus faecium were the most common species isolated.…”

Section: Plos Onesupporting

confidence: 61%

Disposal habits and microbial load of solid medical waste in sub-district healthcare facilities and households in Yilo-Krobo municipality, Ghana

et al. 2021

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The study aimed to assess disposal practices and quantify the microbial load present in SMW from ten sub-district level healthcare facilities and 385 households in Yilo Krobo municipality, Ghana. Disposal of solid medical waste (SMW) was assessed by questionnaire-based surveys, unstructured interviews and field observations. Microbiological analysis identified species and counts of bacteria present in SMW from both sources. Sociodemographic factors influencing the method of SMW disposal in households were evaluated using logistic regression analysis, with statistical significance set at p<0.05. Open burning (29%), burying (25%) and disposal at a dumpsite (49%) were common methods used by households to discard SMW. SMW disposal at a dumpsite was associated with age of respondents in households. Older people (50+ years) were three times more likely to place SMW in household waste later discarded at a dumpsite, compared to younger persons (20–30 years) [a0R, 95%CI = 3.37, 1.41–8.02]. In sub-district level healthcare facilities, open burning and burying were the most common methods used. Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa, Clostridium tetani, Enterococcus faecalis, Acinetobacter spp. Escherichia coli, Bacillus cereus and Enterococcus faecium) were bacteria identified in SMW recovered from both the healthcare facilities and the households. Klebsiella pneumoniae, Acinetobacter spp. and Clostridium tetani were found exclusively in untreated SMW generated in the healthcare facilities. Bacillus spp. and Pseudomonas spp. were found in one sample of treated SMW. The microbial load in SMW from healthcare facilities and households ranged from 0.036 x 103cfc/mg to 0.167 x 103 cfc/mg and from 0.118 x 103cfc/mg to 0.125 x 103cfc/mg respectively. This highlights the need for institutionalizing appropriate treatment methods in sub-district level facilities or strengthening the linkages with higher level facilities to ensure regular and adequate treatment of SMW. Public guidance on management of SMW generated in households which is context specific should also be provided.

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Section: Plos Onesupporting

confidence: 61%

Disposal habits and microbial load of solid medical waste in sub-district healthcare facilities and households in Yilo-Krobo municipality, Ghana

et al. 2021

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Biology of Killer Yeast and Technological Implications

Bajaj

Singh

2017

Yeast Diversity in Human Welfare

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The Prevalence of Killer Yeasts in the Gardens of Fungus-Growing Ants and the Discovery of Novel Killer Toxin named Ksino

Bizarria,

Creagh,

Badigian

et al. 2024

Preprint

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Killer toxins are proteinaceous antifungal molecules produced by yeasts, with activity against a wide range of human and plant pathogenic fungi. Fungus gardens of attine ants in Brazil were surveyed to determine the presence of killer toxin-producing yeasts and to define their antifungal activities and ecological importance. Our results indicate that up to 46% of yeasts isolated from specific fungal gardens can be killer yeasts, with an overall prevalence of 17% across all strains tested. Killer yeasts were less likely to inhibit the growth of yeasts isolated from the same environment but more effective at inhibiting yeast isolated from other environments, supporting a role for killer yeasts in shaping community composition. All killer yeasts harbored genome-encoded killer toxins due to the lack of cytoplasmic toxin-encoding elements (i.e., double-stranded RNA satellites and linear double-stranded DNAs). Of all the killer yeasts identified, an isolate of Candida sinolaborantium showed a broad spectrum of antifungal activities against 57% of yeast strains tested for toxin susceptibility. The complete genome sequence of C. sinolaborantium identified a new killer toxin, Ksino, with primary and tertiary structure homology to the Saccharomyces cerevisiae killer toxin named Klus. Genome-encoded homologs of Ksino were found in yeast strains of Saccharomycetes and Pichiomycetes, as well as other species of Ascomycota and Basidiomycota filamentous fungi. This demonstrates that killer yeasts can be widespread in attine ant fungus gardens, possibly influencing fungal community composition and the importance of these complex microbial communities for discovering novel antifungal molecules.

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Typing of Staphylococcus epidermidis clinical strains by a selected panel of Brazilian killer yeasts

Cited by 5 publications

References 23 publications

Disposal habits and microbial load of solid medical waste in sub-district healthcare facilities and households in Yilo-Krobo municipality, Ghana

Disposal habits and microbial load of solid medical waste in sub-district healthcare facilities and households in Yilo-Krobo municipality, Ghana

Biology of Killer Yeast and Technological Implications

The Prevalence of Killer Yeasts in the Gardens of Fungus-Growing Ants and the Discovery of Novel Killer Toxin named Ksino

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