Typing of porcine circovirus in clinical specimens by multiplex PCR

Detection of PCV2 DNA by SYBR Green I-based quantitative PCR

“…In addition, PCV2 was found to be associated with porcine dermatitis and nephropathy syndrome (PDNS) (Wellenberg et al, 2004), respiratory disease complex (Kim and Chae, 2003) and reproduction failure (Sanchez et al, 2001). Several qualitative PCR methods have been described for the detection of PCV2 in fresh and fixed material (Allan et al, 1999;Hamel et al, 2000;Kim and Chae, 2003;Larochelle et al, 1999;Ouardani et al, 1999). However, as PCV2 is so common within the swine population that conventional PCR can only be used as reference for PMWS diagnosis (Brunborg et al, 2004).…”

Monitoring for Presence of Potentially Xenotic Viruses in Recipients of Pig Islet Xenotransplantation

“…Recipient monitoring has included testing peripheral blood mononuclear cells (PBMC) and blood plasma for PCV 2 (19), PLHV (7), PCMV (2,11,13), and PERV by PCR or reverse transcription-PCR (25). This testing has been carried out on each recipient before transplantation, at nine intervals during the first year posttransplantation, and once each subsequent year.…”

PMWS-like Lesions in Preweaned Crossbred Piglets Naturally Infected with Porcine Circovirus 2 in India

“…Following DNA extraction, PCR targeting the ORF 2 gene of PCV2 was carried out using previously published primers which amplify a 264 bp product (Larochelle et al, 1999). The tissues were also screened by PCR/RT-PCR for the presence of Porcine Parvovirus (PPV) (Arnauld et al, 1998), Porcine Reproductive and Respiratory Syndrome virus (PRRSV) (Mardassi et al, 1994;Suarez et al, 1994), Aujeszky's Disease virus (ADV) (Balasch et al, 1998) and Classical Swine Fever virus (CSFV) (Katz et al, 1993).…”

“…Dig-11-dUTP labeled probe of 264 bp was generated by PCR using primers targeting the ORF 2 gene of PCV2 (Larochelle et al, 1999). Hybridization was carried out at 59 o C for 10 hours in a slide hybridizer (Dako, Denmark).…”