Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Salomonsen, Charlotte Mark; Themudo, Gonçalo Espregueira; Jelsbak, Lars; Molin, Søren; Høiby, Niels; Hammer, Anne Sofie

doi:10.1016/j.vetmic.2012.12.003

Cited by 24 publications

(18 citation statements)

References 29 publications

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“…Mink are the only animal species known to develop acute, contagious and fatal hemorrhagic pneumonia caused by P. aeruginosa . However, to date, no underlying reasons have been identified [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a “phiKMV-Like” Bacteriophage and Its Therapeutic Effect on Mink Hemorrhagic Pneumonia

et al. 2015

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The objective of this study was to investigate the potential of using phages as a therapy against hemorrhagic pneumonia in mink both in vitro and in vivo. Five Pseudomonas aeruginosa (P. aeruginosa) strains were isolated from lungs of mink with suspected hemorrhagic pneumonia and their identity was confirmed by morphological observation and 16S rDNA sequence analysis. Compared to P. aeruginosa strains isolated from mink with hemorrhagic pneumonia in 2002, these isolates were more resistant to antibiotics selected. A lytic phage vB_PaeP_PPA-ABTNL (PPA-ABTNL) of the Podoviridae family was isolated from hospital sewage using a P. aeruginosa isolate as host, showing broad host range against P. aeruginosa. A one-step growth curve analysis of PPA-ABTNL revealed eclipse and latent periods of 20 and 35 min, respectively, with a burst size of about 110 PFU per infected cell. Phage PPA-ABTNL significantly reduced the growth of P. aeruginosa isolates in vitro. The genome of PPA-ABTNL was 43,227 bp (62.4% G+C) containing 54 open reading frames and lacked regions encoding known virulence factors, integration-related proteins and antibiotic resistance determinants. Genome architecture analysis showed that PPA-ABTNL belonged to the “phiKMV-like Viruses” group. A repeated dose inhalational toxicity study using PPA-ABTNL crude preparation was conducted in mice and no significantly abnormal histological changes, morbidity or mortality were observed. There was no indication of any potential risk associated with using PPA-ABTNL as a therapeutic agent. The results of a curative treatment experiment demonstrated that atomization by ultrasonic treatment could efficiently deliver phage to the lungs of mink and a dose of 10 multiplicity of infection was optimal for treating mink hemorrhagic pneumonia. Our work demonstrated the potential for phage to fight P. aeruginosa involved in mink lung infections when administered by means of ultrasonic nebulization.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a “phiKMV-Like” Bacteriophage and Its Therapeutic Effect on Mink Hemorrhagic Pneumonia

et al. 2015

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“…Both indistinguishable and very close PFGE profiles were also found among mink P. aeruginosa from different regions in Eastern China [ 10 ], suggesting that certain P. aeruginosa clones, similarly to humans, would be more pathogenic to animals than others. These clones may spread either through a food or water supply, or may persist in the environment, as demonstrated for P. aeruginosa infecting mink [ 4 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Pseudomonas aeruginosa is a major opportunistic human pathogen, causing infections mostly in patients with impaired immune systems [ 1 ], or in people suffering from cystic fibrosis. It is also a cause of diseases in both livestock and companion animals, including otitis and urinary tract infections in dogs, mastitis in dairy cows, endometritis in horses and hemorrhagic pneumoniae in fur-bearing animals such as mink or foxes [ 2 - 4 ].…”

Section: Introductionmentioning

confidence: 99%

Population structure and antimicrobial susceptibility of Pseudomonas aeruginosa from animal infections in France

Haenni¹,

Hocquet

Ponsin³

et al. 2015

BMC Vet Res

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BackgroundPseudomonas aeruginosa is a major human pathogen, which also affects animals. It is thought that P. aeruginosa has a non-clonal epidemic population structure, with distinct isolates found in humans, animals or the environment. However, very little is known about the structure of the P. aeruginosa population from diseased animals. Data on antimicrobial resistance are also scarce.ResultsThirty-four already registered and 19 new MLST profiles were identified. Interestingly, a few clones were more prevalent, and clones associated to human outbreaks were also detected. Multidrug resistance phenotypes were overall rare.ConclusionWe highlight the non clonal structure of the population and show a higher prevalence of specific clones, possibly correlating with higher pathogenicity. The low proportion of antimicrobial resistance contrasts with the high resistance rate of human isolates.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0324-x) contains supplementary material, which is available to authorized users.

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“…It is also one of the major cause of diseases such as otitis, mastitis, endometritis, hemorrhagic pneumonia and urinary tract infections in both livestock and companion animals (Kidd et al 2011; Poonsuk and Chuanchuen 2012; Salomonsen et al 2013). However, in comparison with reported cases of hospital acquired infections, community associated infection due to P. aeruginosa is rarely reported (Huhulescu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic susceptibility pattern and analysis of plasmid profiles of Pseudomonas aeruginosa from human, animal and plant sources

et al. 2016

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Multidrug resistant organisms (MDROs) constitute a major public health threat globally. Clinical isolates of Pseudomonas aeruginosa remains one of the most studied MDROs however there is paucity of information regarding the susceptibility of its animal and plants isolates to antipseudomonas drug in Nigeria. From a total of 252 samples consisting of plants, animals and clinical samples, 54, 24 and 22 P. aeruginosa were isolated from vegetables, animals and clinical sources respectively. All the isolates were identified by standard biochemical methods. Antimicrobial susceptibility testing (AST) of the 100 P. aeruginosa isolates against 7 antipseudomonal drugs was carried out by disk diffusion method, the phenotypic detection of ESBL was done by double disk synergy test (DDST) while plasmid extraction on 20 selected isolates based on their resistance to 2 or more classes of antibiotics was carried out by alkaline lysis method and analysed with Lambda DNA/Hind lll marker respectively. The AST results revealed highest resistance of 91 and 55 % to ceftazidime and carbenicillin respectively while highest susceptibilities of 99 % for piperacillin–tazobactam and imipenem were recorded in overall assay. Fifteen out of 100 isolates specifically (10) from vegetables, (3) clinical and (2) poultry isolates showed synergy towards the beta-lactamase inhibitor indicating production of ESBL by DDST method. Detection of plasmids was among vegetable (n = 4), poultry (n = 4), cow (n = 3) and clinical isolates (n = 1). Plasmid profile for the selected isolates revealed 6 of the strains had one plasmids each while 5 strains possessed 2–4 plasmids and 1 strain had 5 plasmids. The sizes of the plasmid range from <1 to ≥23kbp. Detection of ESBL and Plasmids among the investigated isolates is suggestive of multiple interplay of resistance mechanism among the isolates. Plants and animal isolates of P. aeruginosa harbouring multiple mechanisms of resistance is of concern due to the danger it poses on the public health.

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Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Cited by 24 publications

References 29 publications

Isolation and Characterization of a “phiKMV-Like” Bacteriophage and Its Therapeutic Effect on Mink Hemorrhagic Pneumonia

Isolation and Characterization of a “phiKMV-Like” Bacteriophage and Its Therapeutic Effect on Mink Hemorrhagic Pneumonia

Population structure and antimicrobial susceptibility of Pseudomonas aeruginosa from animal infections in France

Antibiotic susceptibility pattern and analysis of plasmid profiles of Pseudomonas aeruginosa from human, animal and plant sources

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