Typing of strains from a single-source outbreak of Pseudomonas pickettii

Abstract

Plasmid profiles, genome restriction fragment polymorphisms, carbohydrate oxidation-fermentation reactions, methylumbelliferyl substrate hydrolysis patterns, antimicrobial susceptibilities, and results obtained with the Biolog GN biochemical substrate kit were used to type 19 common-source, but mixed-biotype, outbreak strains and one epidemiologically distinct strain ofPseudomonas pickettii. Biotyping with conventional and methylumbelliferyl substrates failed to distinguish between strains. Plasmid profile testing was found to be inconsistent and not reproducible. The Biolog GN kit allowed greater strain differentiation than restriction fragment polymorphism did (12 biotypes versus 5 biotypes); antimicrobial susceptibility testing yielded 4

Native Valve Endocarditis due toRalstonia pickettii: A Case Report and Literature Review

“…Historically, the first documented case of Ralstonia bacteremia and death was reported in 1968 [ 7 ]. At that time, the pathogen was reported as an unclassified, Gram-negative bacterium (Group IV d) which was only later identified as Ralstonia pickettii [ 8 ]. The case was a 33-year-old African American male who had persistent positive blood cultures with a Group IV d Gram-negative bacillus resistant to all attempted antibiotics (ampicillin, penicillin G, and chloramphenicol).…”

Characterization of regulatory element Rp3 of regulation of β-lactamases fromRalstonia pickettii

“…Ralstonia pickettii is a nonfermenting opportunistic pathogen, commonly grouped with and referred to as Burkholderia cepacia-like organisms. All species belonging to this group (Burkholderia gladioli, Pandoraea and Ralstonia) are capable of colonizing cystic fibrosis patients and may be involved in nosocomial infections (Kahan et al, 1983;Vershraegen et al, 1985;Dimech et al, 1993;Raveh et al, 1993;Chen et al, 1995;Chetoui et al, 1997). Ralstonia pickettii emerged recently as a new pathogen in a hospital in Rio de Janeiro, causing bloodstream infections, which originated from a contaminated intravenous solution and from colonized medical devices (Pellegrino et al, 2008).…”