Tyropeptins, proteasome inhibitors produced by Kitasatospora sp. MK993-dF2

Momose, Isao; Watanabe, Takumi

doi:10.1038/ja.2017.9

Cited by 11 publications

(11 citation statements)

References 79 publications

(81 reference statements)

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“…Thus, a general reduction of proteasomal activity has an effect on chloroplast protein abundance that is different from the rpn8a mutation. This is not surprising because the 20S proteasome removes damaged proteins under conditions of oxidative stress 9 and inhibition of its activity by MG132 interferes with this important function 25 .…”

Section: Resultsmentioning

confidence: 99%

“…Dried eluates were resuspended in 45 μl of 0.1% (w/v) TFA and 1/3rd of each fraction applied was analyzed by nano-LC-MS/MS using a Orbitrap Fusion Lumos mass spectrometer online-coupled to a nano RSLC HPLC system (both Thermo Scientific). LC separation was performed as described before 25 . Samples were measured in data-dependent acquisition mode using the top speed option (3 s).…”

Section: Methodsmentioning

confidence: 99%

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Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

et al. 2020

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The proteasome is an essential protein-degradation machinery in eukaryotic cells that controls protein turnover and thereby the biogenesis and function of cell organelles. Chloroplasts import thousands of nuclear-encoded precursor proteins from the cytosol, suggesting that the bulk of plastid proteins is transiently exposed to the cytosolic proteasome complex. Therefore, there is a cytosolic equilibrium between chloroplast precursor protein import and proteasomal degradation. We show here that a shift in this equilibrium, induced by mild genetic proteasome impairment, results in elevated precursor protein abundance in the cytosol and significantly increased accumulation of functional photosynthetic complexes in protein import-deficient chloroplasts. Importantly, a proteasome lid mutant shows improved photosynthetic performance, even in the absence of an import defect, signifying that functional precursors are continuously degraded. Hence, turnover of plastid precursors in the cytosol represents a mechanism to constrain thylakoid membrane assembly and photosynthetic electron transport.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

et al. 2020

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“…With this background in mind, the author carried out synthetic studies on biologically active natural products and their related compounds. In this review, studies on leptolyngbyolides (cytotoxic marine macrolides), 83,84) BE-54017 (V-ATPase inhibitor), 85) tyropeptin (proteasome inhibitor), [86][87][88][89] androprostamine A and resormycin (antiproliferative agents against prostate cancer cells) 90) are not described, although the details can be found in the publications indicated. Synthetic chemistry and natural products have long been friends and provided many opportunities to create medicines which in turn improved public health.…”

Section: Discussionmentioning

confidence: 99%

Chemical Studies on Bioactive Natural Products Directed toward Development of Novel Antiinfective and Anticancer Medicines

Watanabe

2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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Natural products are still rich sources of clinically used medicines and lead compounds for them. This review summarizes chemical studies carried out by the author on natural products of microorganism origin, many of which were discovered at the Institute of Microbial Chemistry (BIKAKEN). Caprazamycin B is a liponucleoside antibiotic from which CPZEN-45, an antituberculosis agent with a unique mode of action, was developed. Intervenolin and leucinostatin A exert antiproliferative activity toward tumor cells in the presence of the corresponding stromal cells, which implies that the primary molecular targets of these molecules should be related to growth signals from normal (stromal) cells. Details of the endeavors to establish efficient synthetic routes to these compounds which accelerated structure-activity relationship studies and further evaluation of biological activity are described.

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“…Tyropeptin A and B, isolated from Kitasatospora sp. MK993‐dF2, have also shown potential to inhibit the CT‐L activity of proteasome and reduce growth in prostate cancer cells . Another molecule known as Syringolin A, secreted by Pseudomonas syringae pv.…”

Section: Recent Functional Modulation Of Proteasome: Insights May Ovementioning

confidence: 99%

“…MK993-dF2, have also shown potential to inhibit the CT-L activity of proteasome and reduce growth in prostate cancer cells. 520,521 Another molecule known as Syringolin A, secreted by Pseudomonas syringae pv. syringae inhibits all three proteolytic activities of proteasome.…”

Section: Can Natural Molecules Overcome the Challenges Of Proteasome?mentioning

confidence: 99%

Proteasome‐mediated proteostasis: Novel medicinal and pharmacological strategies for diseases

Mishra

Upadhyay

Prajapati

et al. 2018

Medicinal Research Reviews

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Proteins actively participate in a wide range of cellular physiological functions. But aggregation of proteins results in cytotoxicity, and unwanted aggregation of misfolded proteins often causes many diseases. During abnormal protein aggregation events, cells try to cope against such deleterious consequences because of the remarkable functional attempts of two distinct proteolytic mechanisms. These tightly regulative and signaling mechanisms are autophagy pathway and ubiquitin proteasome system. Proteasome complex system holds the elimination capacity of intracellular aberrant protein aggregation. Despite the considerable progress that has been achieved, which elucidates wide function and diverse roles of proteasome system, still several crucial problems remain unanswered. For example, how the complex proteasomes assembly and their interactive pathways determine the precise sense of several proteotoxic insults, which can severely affect the cell survival and homeostasis? The specific degradation of various aberrant proteins that can disturb cellular homeostasis is achieved by proper proteasome functionality, which is yet another unclear and critical challenge. Therefore, a better understanding of the various cellular signaling mechanisms composing the proteasome machinery carries broad therapeutic implications linked with proteopathies. This article signifies the urgent need, which is now crucial for us to improve our understanding of the proteasome architecture, structure, and functions that span multiple level strategies from the molecular level to the cellular level. This systematic in-depth information of proteasome may be helpful in the near future to design a new molecular framework based on intrinsic and extrinsic cellular mechanisms that drive the assembly of proteasome to induce cellular survival against proteostasis imbalance and disease conditions.

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Tyropeptins, proteasome inhibitors produced by Kitasatospora sp. MK993-dF2

Cited by 11 publications

References 79 publications

Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

Chemical Studies on Bioactive Natural Products Directed toward Development of Novel Antiinfective and Anticancer Medicines

Proteasome‐mediated proteostasis: Novel medicinal and pharmacological strategies for diseases

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