Tyrosinase based amperometric biosensor for determination of tyramine in fermented food and beverages with gold nanoparticle doped poly(8-anilino-1-naphthalene sulphonic acid) modified electrode

Silva, Wanderson da; Ghica, Mariana Emilia; Ajayi, Rachel Fanelwa; Iwuoha, Emmanuel I.; Brett, Christopher M.A.

doi:10.1016/j.foodchem.2018.12.104

Cited by 67 publications

(28 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AuNPs have gained attention during the last decades because of their unique properties, like Surface Plasmon Resonance (SPR), non-toxicity, biocompatibility and stability, which have made them a very attractive material for biosensor systems [5][6][7][8]. Moreover, many researchers have investigated the role of AuNPs in the areas of electronics [9,10], biomedical [11,12], catalytic [13][14][15], food technology [16][17][18] and also solar cells for high performance applications [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Low-cost synthesis of AuNPs through ultrasonic spray pyrolysis

Tiyyagura

Majerič

Anžel

et al. 2020

Mater. Res. Express

View full text Add to dashboard Cite

The present research informs about the synthesis of gold nanoparticles (AuNPs) through Ultrasonic Spray Pyrolysis (USP), which were collected in ethanol with 0.1% Polyvinylpyrrolidone (PVP). Initially, the research focused on two precursors, where the first represented a homemade H-HAuCl 4 , completed in our own laboratory through the chlorine gas method by using HCl and KMnO 4 , and the second was the commercial C-HAuCl 4 , prepared by using Gold (III) chloride tetrahydrate powder and deionised water. The goal was to find any potential precursor differences and their influences on the later use for AuNPs synthesis through USP using almost the same parameters. In the first step of research it was determined that the H-HAuCl 4 precursor was similar to C-HAuCl 4 in chemical composition, surface tension and pH value. This finding represented the starting point for being able to use H-HAuCl 4 in the USP for AuNPs' synthesis. In the second step, AuNPs were synthesised from both types of precursors. Afterwards, characterisation of some functional properties by FTIR and UVvis techniques was done directly for H-and C-AuNPs in the collecting media. For SEM/EDX and TEM microscopy both types of H-and C-AuNPs were dried, and observation revealed that the morphology, shape and size distribution of dried AuNPs were very similar. Based on the performed laboratory research, it could be concluded that prepared H-AuNPs could represent a new and lowcost effective solution for future USP transfer onto the industrial level, not only in in the process itself, but also in the field of Low-cost Precursor Preparation.Abbreviations

show abstract

Section: Introductionmentioning

confidence: 99%

Low-cost synthesis of AuNPs through ultrasonic spray pyrolysis

Tiyyagura

Majerič

Anžel

et al. 2020

Mater. Res. Express

View full text Add to dashboard Cite

show abstract

“…They can also be coupled to different devices to perform in situ analyzes, using different detection methods [ 34 ], especially voltammetric methods. Laccase and tyrosinase biosensors, depending on their specificity, are used for the detection of phenolic compounds in the food industry [ 35 , 36 ], in the analysis of the environment [ 37 , 38 , 39 ] or biological samples [ 40 , 41 ]. The activity of these enzymes in the sensitive element of the biosensor, involves the binding and cleavage of molecular oxygen in the active center of enzyme, which include copper ions.…”

Section: Introductionmentioning

confidence: 99%

Laccase and Tyrosinase Biosensors Used in the Determination of Hydroxycinnamic Acids

Bounegru

Apetrei

2021

IJMS

View full text Add to dashboard Cite

In recent years, researchers have focused on developing simple and efficient methods based on electrochemical biosensors to determine hydroxycinnamic acids from various real samples (wine, beer, propolis, tea, and coffee). Enzymatic biosensors represent a promising, low-cost technology for the direct monitoring of these biologically important compounds, which implies a fast response and simple sample processing procedures. The present review aims at highlighting the structural features of this class of compounds and the importance of hydroxycinnamic acids for the human body, as well as presenting a series of enzymatic biosensors commonly used to quantify these phenolic compounds. Enzyme immobilization techniques on support electrodes are very important for their stability and for obtaining adequate results. The following sections of this review will briefly describe some of the laccase (Lac) and tyrosinase (Tyr) biosensors used for determining the main hydroxycinnamic acids of interest in the food or cosmetics industry. Considering relevant studies in the field, the fact has been noticed that there is a greater number of studies on laccase-based biosensors as compared to those based on tyrosinase for the detection of hydroxycinnamic acids. Significant progress has been made in relation to using the synergy of nanomaterials and nanocomposites for more stable and efficient enzyme immobilization. These nanomaterials are mainly carbon- and/or polymer-based nanostructures and metallic nanoparticles which provide a suitable environment for maintaining the biocatalytic activity of the enzyme and for increasing the rate of electron transport.

show abstract

“…For this reason, several GC works involve surface functionalization schemes for biosensing by decorating the material´s surface by solvothermal deposition [ 30–35 ] and by electrodeposition in solution. [ 35,36 ] In particular, noble‐metal nanoparticles (NPs), either by doping of or surface attachment to carbon films, have been extensively studied [ 35,37–41 ] because of their versatility, chemical stability, [ 42,43 ] and unique optical properties. [ 44 ] These NPs have also found application in microelectromechanical devices such as sensors, [ 45 ] medical devices.…”

Section: Introductionmentioning

confidence: 99%