Tyrosinase-immobilized CNT based biosensor for highly-sensitive detection of phenolic compounds

Wee, Youngho; Park, Seunghwan; Kwon, Young Hyeon; Ju, Youngjun; Yeon, Kyung Min; Kim, Jungbae

doi:10.1016/j.bios.2019.03.008

Cited by 85 publications

(30 citation statements)

References 43 publications

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“…They applied Carbon Nanotubes (CNTs) to make a solution of Enzyme Adsorption, Participation and Crosslinking (EAPC) by crosslinking with tyrosinase molecules. Using these nanostructures, the sensitivity of the sensor was improved, as the LOD for phenol and catechol reached 35 and 14 nM, respectively [ 119 ]. Bhardwaj et al developed a SPR-based biosensor using antibodies to detect AFB1.…”

Section: Emerging Biosensor-based Detection Methods Of Natural Producmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities make the detection, determination or quantification of NPs essential for their application. The advanced instrumental methods require time-consuming sample preparation and analysis. In contrast, biosensors allow rapid detection of NPs, especially in complex media, and are the preferred choice of detection when speed and high throughput are intended. Here, we review diverse biosensors reported for the detection of NPs. The emerging approaches for improving the efficiency of biosensors, such as microfluidics, nanotechnology, and magnetic beads, are also discussed. The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices. The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells, sensing elements, and detection mechanisms on miniaturized folded chips.

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Section: Emerging Biosensor-based Detection Methods Of Natural Producmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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“…Ty-based electrochemical biosensors may be considered “classics” because Tyr is an enzyme suitable for evaluating the electrochemical properties of materials, with the help of which new nanostructured electrodes are developed [ 35 , 115 , 116 ]. Nanomaterials designed to improve the performance of tyrosinase-based biosensors include carbon nanostructures (e.g., carbon nanotubes, graphene, carbon quantum dots, and nanodiamonds), metal nanoparticles, and oxides (e.g., gold, platinum, and nickel oxide nanoparticles) and semiconductor nanoparticles (e.g., CdS quantum dots and titanium nanoparticles) [ 16 , 39 , 117 , 118 , 119 ].…”

Section: Enzymatic Biosensors For the Detection Of Hydroxycinnamic Acidsmentioning

confidence: 99%

“…They can also be coupled to different devices to perform in situ analyzes, using different detection methods [ 34 ], especially voltammetric methods. Laccase and tyrosinase biosensors, depending on their specificity, are used for the detection of phenolic compounds in the food industry [ 35 , 36 ], in the analysis of the environment [ 37 , 38 , 39 ] or biological samples [ 40 , 41 ]. The activity of these enzymes in the sensitive element of the biosensor, involves the binding and cleavage of molecular oxygen in the active center of enzyme, which include copper ions.…”

Section: Introductionmentioning

confidence: 99%

Laccase and Tyrosinase Biosensors Used in the Determination of Hydroxycinnamic Acids

Bounegru

Apetrei

2021

IJMS

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In recent years, researchers have focused on developing simple and efficient methods based on electrochemical biosensors to determine hydroxycinnamic acids from various real samples (wine, beer, propolis, tea, and coffee). Enzymatic biosensors represent a promising, low-cost technology for the direct monitoring of these biologically important compounds, which implies a fast response and simple sample processing procedures. The present review aims at highlighting the structural features of this class of compounds and the importance of hydroxycinnamic acids for the human body, as well as presenting a series of enzymatic biosensors commonly used to quantify these phenolic compounds. Enzyme immobilization techniques on support electrodes are very important for their stability and for obtaining adequate results. The following sections of this review will briefly describe some of the laccase (Lac) and tyrosinase (Tyr) biosensors used for determining the main hydroxycinnamic acids of interest in the food or cosmetics industry. Considering relevant studies in the field, the fact has been noticed that there is a greater number of studies on laccase-based biosensors as compared to those based on tyrosinase for the detection of hydroxycinnamic acids. Significant progress has been made in relation to using the synergy of nanomaterials and nanocomposites for more stable and efficient enzyme immobilization. These nanomaterials are mainly carbon- and/or polymer-based nanostructures and metallic nanoparticles which provide a suitable environment for maintaining the biocatalytic activity of the enzyme and for increasing the rate of electron transport.

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“…In the last few years, a number of electrochemical biosensors combining phenol oxidases with a variety of materials, such as polymeric matrices [5,6] or nanomaterials, Sensors 2021, 21, 899 2 of 13 have been developed [7,8]. Carbon nanotubes (CNTs) [9,10] graphene [11,12], carbon fibers [13], metallic nanoparticles, metal oxide nanoparticles, nanostars [14][15][16][17][18], molecular imprinted polymers [19], and nanostructured thin films [20,21] have all been shown to be excellent electron mediators. One-dimensional (1D) nanostructures can be an interesting alternative to zero-dimensional (OD) nanoparticles due to their high surface-to-volume ratios.…”

Section: Introductionmentioning

confidence: 99%

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Salvo-Comino

Martín-Pedrosa

Garcı́a

et al. 2021

Sensors

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The integration of nanomaterials as electron mediators in electrochemical biosensors is taking on an essential role. Due to their high surface-to-volume ratio and high conductivity, metallic nanowires are an interesting option. In this paper, silver nanowires (AgNWs) were exploited to design a novel catechol electrochemical biosensor, and the benefits of increasing the aspect ratio of the electron mediator (nanowires vs. nanoparticles) were analyzed. Atomic force microscopy (AFM) studies have shown a homogeneous distribution of the enzyme along the silver nanowires, maximizing the contact surface. The large contact area promotes electron transfer between the enzyme and the electrode surface, resulting in a Limit of Detection (LOD) of 2.7 × 10−6 M for tyrosinase immobilized onto AgNWs (AgNWs-Tyr), which is one order of magnitude lower than the LOD of 3.2 × 10−5 M) obtained using tyrosinase immobilized onto silver nanoparticles (AgNPs-Tyr). The calculated KM constant was 122 mM. The simultaneous use of electrochemistry and AFM has demonstrated a limited electrochemical fouling that facilitates stable and reproducible detection. Finally, the biosensor showed excellent anti-interference characteristics toward the main phenols present in wines including vanillin, pyrogallol, quercetin and catechin. The biosensor was able to successfully detect the presence of catechol in real wine samples. These results make AgNWs promising elements in nanowired biosensors for the sensitive, stable and rapid voltammetric detection of phenols in real applications.

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Tyrosinase-immobilized CNT based biosensor for highly-sensitive detection of phenolic compounds

Cited by 85 publications

References 43 publications

Emerging biosensors in detection of natural products

Emerging biosensors in detection of natural products

Laccase and Tyrosinase Biosensors Used in the Determination of Hydroxycinnamic Acids

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

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