Tyrosinase Immobilized on Magnetic Nanoparticles for Ligand Fishing of Tyrosinase Ligands from Prunella Vulgaris L

Yan, Zhao; Li, Yuan; Bai, Xiao-Bo; Jiang, Xin-Xin; Zhang, Yi; Fang, Qiong; Zhang, Qin; Liao, Xun

doi:10.2139/ssrn.4057236

Cited by 1 publication

(2 citation statements)

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Section: Resultsmentioning

confidence: 99%

“…In humans, tyrosinase is closely related to melanogenesis and its inhibitors may represent an important clinical strategy to treat melanoma [88]. In this sense, tyrosinase inhibitors were sought by different ligand fishing approaches such as HF [42], UF [19,[88][89][90][91][92][93][94], and MPs [95,96], in an attempt to efficiently capture ligands with affinity for this target. Yang et al [89], for example, successfully fished out two derivatives from quercetin and kaempferol (quercetin-3-O-(6-O-malonyl)-β-d-glucopyranoside and kaempferol-3-O-(6-O-malonyl)-β-D-glucopyranoside, respectively) from the Morus alba L. leaf extract as promising tyrosinase inhibitors.…”

Section: Tyrosinasementioning

confidence: 99%

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Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential.

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