Tyrosinase-mediated melanogenesis in melanoma cells: Array comparative genome hybridization integrating proteomics and bioinformatics studies

Yin, Sheng; Lee, Jae-Rin; Hahn, Myong‐Joon; Yang, Jun‐Mo; Qian, Guo‐Ying; Park, Yong‐Doo

doi:10.1016/j.ijbiomac.2020.12.146

Cited by 11 publications

(4 citation statements)

References 48 publications

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“…Upregulation of hspa8 (alias hsp70 ) supports this argument. Moreover, hspa8 is a potential diagnostic biomarker for the tyrosinase-mediated melanogenesis process and therefore a minor difference of hspa8 gene expression could be relevant for the transition of RFCs to MFCs (Yin et al 2021 ). Melanogenesis affects gene expression associated with glycolysis and enhances the glycolytic activity of enzymes such as aldolase A (Slominski et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics

Bjørgen,

Malik,

Rimstad

et al. 2023

Cell Tissue Res

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Spatial transcriptomics is a technique that provides insight into gene expression profiles in tissue sections while retaining structural information. We have employed this method to study the pathological conditions related to red and melanized focal changes in farmed Atlantic salmon (Salmo salar). Our findings support a model where similar molecular mechanisms are involved in both red and melanized filet discolorations and genes associated with several relevant pathways show distinct expression patterns in both sample types. Interestingly, there appears to be significant cellular heterogeneity in the foci investigated when looking at gene expression patterns. Some of the genes that show differential spatial expression are involved in cellular processes such as hypoxia and immune responses, providing new insight into the nature of muscle melanization in Atlantic salmon.

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Section: Discussionmentioning

confidence: 99%

Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics

Bjørgen,

Malik,

Rimstad

et al. 2023

Cell Tissue Res

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“…Including, ACTB as a hub protein associated with tyrosinase-mediated melanogenesis in melanoma cells [34]. The research results suggested that ACTB was involved in the formation of melanin in melanocyte or melanoma cells.…”

Section: A C C E P T E D a R T I C L Ementioning

confidence: 85%

Skin proteomic screening and functional analysis of differential proteins associated with coat color in sheep (Ovis aries)

Yin,

Hao,

Zhao

et al. 2024

Anim Biosci

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Objective: Coat color is an important characteristic and economic trait in domestic sheep. In this study, we explored the potential mechanisms and the signaling pathways involved in coat color regulation for sheep. Methods: Isobaric tags for relative and absolute quantification (iTRAQ) technology was used to catalog global protein expression profiles in skin of sheep with black versus white coat color. Immunofluorescence was used to observe the expression localization of differential protein. Western blot and quantitative real time polymerase chain reaction (qRT-PCR) were used to evaluate their role in the coat color formation of sheep. Results: A total of 136 differential proteins were obtained in different coat colors, including 101 up-regulated and 35 down-regulated. Pigmentation function entries were enriched through GO annotation. Tyrosine metabolism and platelet activation signaling pathway were extracted by KEGG analysis. APOA1 (Apolipoprotein A-1)and FGA (Fibrinogen alpha chain) were found to be critical differential proteins by the interaction of differential proteins in the direct-interaction network diagram.Strikingly, twenty candidate differential proteins were screened, from which ACTB (Beta-actin) protein showed higher expression in white sheep skin, while ALB (albumin), APOA1 MAOA (Amine oxidase) and FGA proteins showed higher expression in black sheep skin, which validated by immunofluorescence, western blot and qRT-PCR. Conclusion:Our studies identified several novel proteins that may involved in A c c e p t e d A r t i c l e the coat color formation of sheep. The white and black sheep skin proteome profiles obtained provide a valuable resource for future research to understand the network of protein expression controlling skin physiology and melanogenesis in sheep.

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“…EGFR is a type I tyrosine kinase receptor, which is expressed on the membrane surface of keratinocytes and melanocytes to activate the intracellular kinase pathway, and then to guide the phosphorylation of downstream signaling pathways, such as MAPK, Akt, etc, thereby affecting melanin production 20 . ACTB, a type of actin, is a hub protein target in tyrosinase‐mediated melanogenesis of melanoma cells 21 . In addition, the protein filaments ACT promotes the transport and metabolism of melanin by binding to the N‐terminal power region of MyosinVa.…”

Section: Discussionmentioning

confidence: 99%

A Novel multi‐fruit acids formula design on molecular basis for skin brightening via a system biology approach

Guo¹,

Ma²,

Bouffard³

et al. 2022

J of Cosmetic Dermatology

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Tyrosinase-mediated melanogenesis in melanoma cells: Array comparative genome hybridization integrating proteomics and bioinformatics studies

Cited by 11 publications

References 48 publications

Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics

Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics

Skin proteomic screening and functional analysis of differential proteins associated with coat color in sheep (Ovis aries)

A Novel multi‐fruit acids formula design on molecular basis for skin brightening via a system biology approach

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