2017
DOI: 10.5796/electrochemistry.85.17
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Tyrosinase Modified Poly(thionine) Electrodeposited Glassy Carbon Electrode for Amperometric Determination of Catechol

Abstract: A stepwise strategy of mediator-free amperometric biosensor for the detection of catechol was developed based on the covalent bonding of tyrosinase (TYR) onto thionine (TN)-electrodeposited glassy carbon (GC) surface via glutaraldehyde (GA). Prior to the TYR-immobilization, poly(thionine) was prepared on a GC electrode surface by an electrooxidative polymerization of thionine. The TYR/GA/pTN modified electrode was evaluated by SEM and EIS measurements. The terminal amino groups (-NH 2 ) which electrodeposited … Show more

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Cited by 6 publications
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“…The resulting TYR/GA/pTN/GCE biosensor showed a fast and sensitive response to catechol and a detection limit of 6.0 µM. The biosensor retained 71% of its initial activity for catechol oxidation after one month of storage [ 143 ].…”
Section: Tyrosinase Immobilization Strategiesmentioning
confidence: 99%
“…The resulting TYR/GA/pTN/GCE biosensor showed a fast and sensitive response to catechol and a detection limit of 6.0 µM. The biosensor retained 71% of its initial activity for catechol oxidation after one month of storage [ 143 ].…”
Section: Tyrosinase Immobilization Strategiesmentioning
confidence: 99%