Tyrosine as important contributor to the antioxidant capacity of seminal plasma

Overveld, Floris W.P.C. van; Haenen, Guido R.M.M.; Rhemrev, Johann; Vermeiden, Jan P.W.; Bast, Aalt

doi:10.1016/s0009-2797(00)00179-4

Cited by 83 publications

(38 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The total radical trapping antioxidant potential assay was developed using the ABAP/ABTS reagent. 25 The sample concentrations ranged between 125 and 1000 lg/mL. A sample aliquot of 10 lL was allowed to react with 990 lL of ABTS/ABAP reagent.…”

Section: Content Of Total Anthocyaninsmentioning

confidence: 99%

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Ramos-Escudero

Muñoz

Alvarado-Ortíz

et al. 2012

Journal of Medicinal Food

138

View full text Add to dashboard Cite

This study was designed to determine the contents of total polyphenols, flavonoids, flavonols, flavanols, and anthocyanins of purple corn (Zea mays L.) extracts obtained with different methanol:water concentrations, acidified with 1% HCl (1 N). Another objective was to determine the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and deoxyribose assay, individual phenolic compounds by high-performance liquid chromatography (HPLC), and endogenous antioxidant enzyme (superoxide dismutase [SOD], catalase [CAT], and total peroxidase [TPX]) activity and lipid peroxidation activity (thiobarbituric acidreactive substances [TBARS] assay) in isolated mouse organs. Overall, the highest total content of polyphenols, anthocyanins, flavonoids, flavonols, and flavanols was obtained with the 80:20 methanol:water extract, acidified with 1% HCl (1 N). The 50% inhibitory concentration values obtained by the DPPH and ABTS assays with this extract were 66.3 lg/mL and 250 lg/mL, respectively. The antioxidant activity by the FRAP assay was 26.1 lM Trolox equivalents/g, whereas the deoxyribose assay presented 93.6% inhibition. Because of these results, the 80:20 methanol:water extract, acidified with 1% HCl (1 N), was used for the remaining tests. Eight phenolic compounds were identified by HPLC: chlorogenic acid, caffeic acid, rutin, ferulic acid, morin, quercetin, naringenin, and kaempferol. Furthermore, it was observed that the purple corn extract was capable of significantly reducing lipid peroxidation (lower malondialdehyde [MDA] concentrations by the TBARS assay) and at the same time increasing endogenous antioxidant enzyme (CAT, TPX, and SOD) activities in isolated mouse kidney, liver, and brain. On the basis of the results, it was concluded that the purple corn extract contained various bioactive phenolic compounds that exhibited considerable in vitro antioxidant activity, which correlated well with the decreased MDA formation and increase in activity of endogenous antioxidant enzymes observed in the isolated mouse organs. This warrants further in vivo studies with purple corn extracts to assess its antioxidant activity and other bioactivities.KEY WORDS: antioxidant activity endogenous antioxidant enzymes isolated mouse organs oxidative stress phenolic compounds purple corn Zea mays L.

show abstract

Section: Content Of Total Anthocyaninsmentioning

confidence: 99%

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Ramos-Escudero

Muñoz

Alvarado-Ortíz

et al. 2012

Journal of Medicinal Food

138

View full text Add to dashboard Cite

show abstract

“…In such instances, the fi rst time the spermatozoa will come into contact with infi ltrating leukocytes will be at the moment of ejaculation. At this juncture, the spermatozoa will be protected from extracellular ROS by the powerful antioxidant properties of seminal plasma (Jones et al 1979 ;Rhemrev et al 2000 ;van Overveld et al 2000 ), and the impact of the leukocyte-derived ROS will be minimal. However, quite a different picture emerges when the seminal plasma is removed in the context of preparing spermatozoa for assisted conception therapy.…”

Section: Leukocytes As a Cause Of Oxidative Stressmentioning

confidence: 99%

Damage to Sperm DNA Mediated by Reactive Oxygen Species: Its Impact on Human Reproduction and the Health Trajectory of Offspring

Gavriliouk

Aitken

2015

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Disruptions to the genetic integrity of the mammalian spermatozoon play a major role in determining the subsequent developmental trajectory of the embryo. This chapter examines the causative links that connect DNA damage in human spermatozoa and the appearance of mutations in the progeny responsible for a variety of clinical conditions from autism to cancer. Integral to this discussion is an abundance of evidence indicating that human spermatozoa are vulnerable to free radical attack and the generation of oxidative DNA damage. The resolution of this damage appears to be initiated by the spermatozoa but is driven to completion by the oocyte in a round of DNA repair that follows fertilization. The persistence of unresolved oxidative DNA damage following zygote formation has the potential to create mutations/epimutations in the offspring that may have a profound impact on the health of the progeny. It is proposed that the creation of oxidative stress in the male germ line is a consequence of a wide variety of environmental/lifestyle factors that influence the health and well-being of the offspring as a consequence of mutational change induced by the aberrant repair of oxidative DNA damage in the zygote. Factors such as paternal age, subfertility, smoking, obesity, and exposure to a range of environmental influences, including radio-frequency electromagnetic radiation and xenobiotics, have all been implicated in this process. Identifying the contributors to oxidative stress in the germ line and resolving the mechanisms by which such stressors influence the mutational load carried by the progeny will be an important task for the future. This task is particularly pressing, given the extensive use of assisted reproductive technologies to achieve pregnancies in vitro that would have been prevented in vivo by the complex array of mechanisms that nature has put in place to ensure that only the fittest gametes participate in the generative process.

show abstract

“…Oxidative stress is defined as imbalance between the prooxidant and antioxidant ratio caused by limited availability of fewer antioxidants in relation to excessive amounts of oxidizing agents such as free oxygen radicals. Seminal plasma is rich in several antioxidant molecules (such as glutathione peroxidase, superoxide dismutase, ascorbic acid, alpha-tocopherol, and hypotaurine) secreted by the male reproductive tract [36,37] that protect the sperm from the oxidative stress caused by ROS. Presence of high numbers of white blood cells in the semen results in excessive production of ROS which can cause damage to spermatozoa.…”

Section: Oxidative Stressmentioning

confidence: 99%

Role of Assisted Reproduction Techniques in the Management of Unexplained Male Infertility

Gangrade

Patel

2015

Unexplained Infertility

View full text Add to dashboard Cite

Tyrosine as important contributor to the antioxidant capacity of seminal plasma

Cited by 83 publications

References 30 publications

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Damage to Sperm DNA Mediated by Reactive Oxygen Species: Its Impact on Human Reproduction and the Health Trajectory of Offspring

Role of Assisted Reproduction Techniques in the Management of Unexplained Male Infertility

Contact Info

Product

Resources

About