1996
DOI: 10.1016/0378-4347(95)00563-3
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Tyrosine protein kinase assays

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Cited by 9 publications
(5 citation statements)
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“…Numerous protein substrates have been identified for many PTKs; nevertheless, besides autophosphorylation assays, artificial protein substrates such as denatured enolase, and poly(Glu,Tyr) copolymers or peptide substrates (angiotensin or autophosphorylationsite peptide sequences), are commonly used in various PTK assays. 72,73 A thorough review of all the known or putative protein substrates for various protein kinases is outside the scope of this review. However, we will summarize some of the known protein substrates for Src (Table II) to illustrate the wide range of protein substrates this enzyme can recognize (for a review, see Ref.…”
Section: Protein Substrates For Ptkmentioning
confidence: 99%
“…Numerous protein substrates have been identified for many PTKs; nevertheless, besides autophosphorylation assays, artificial protein substrates such as denatured enolase, and poly(Glu,Tyr) copolymers or peptide substrates (angiotensin or autophosphorylationsite peptide sequences), are commonly used in various PTK assays. 72,73 A thorough review of all the known or putative protein substrates for various protein kinases is outside the scope of this review. However, we will summarize some of the known protein substrates for Src (Table II) to illustrate the wide range of protein substrates this enzyme can recognize (for a review, see Ref.…”
Section: Protein Substrates For Ptkmentioning
confidence: 99%
“…Other PTK assay formats, based on either antibody recognition (23)(24)(25)(26) or time-resolved fluorescence (27), have been reported; however, these approaches can also be problematic with respect to the accurate and efficient detection of a broad range of PTK enzymes (28). More recently, the scintillation proximity assay (SPA) has been used to characterize a variety of phenomena relating to PTK signaling, including the interaction of phosphopeptides with the SH 2 domains of p72syk, ZAP70, or p53/p56Lck (29,30), and the interaction of p21ras with either the raf kinase (31, 32) or neurofibromin, a GTPase-activating protein (33).…”
mentioning
confidence: 99%
“…binding of positively charged peptides to negatively charged papers), the same binding considerations will be valid for LSA-50 paper. Hence, we strongly recommend users to pay due attention to optimal peptide substrate design (a step which is often neglected, as shown in a review by Boutin et al) [15].…”
Section: Resultsmentioning
confidence: 99%