Tyrphostin AG 555 Inhibits Bovine Papillomavirus Transcription by Changing the Ratio between E2 Transactivator/Repressor Function

Baars, Sabine; Bachmann, Anastasia; Levitzki, Alexander; Rösl, Frank

doi:10.1074/jbc.m304449200

Cited by 2 publications

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References 67 publications

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“…The E8^E2C fusion protein comprises a short (10 aa) fragment of the E8 ORF fused to the C-terminal DNA binding and dimerization domain of the E2 protein, and has been shown to regulate viral transcription and replication by counteracting E2 functions (McBride, 2013). Additionally, other E2 antagonists have been found in other PVs, such as E2-TR in BPV1 (Choe et al, 1989;Baars et al, 2003). In our case we also found a second ORF, E2*I, which conserves the hinge and DNA-binding domain but lacks the transactivation domain, which would most likely render this protein an E2 repressor.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Mastomys natalensis papillomavirus in productive lesions after natural infection

Salvermoser

Chotewutmontri

Braspenning-Wesch

et al. 2016

Journal of General Virology

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Mastomys coucha, an African rodent, is a useful animal model of papillomavirus infection, as it develops both premalignant and malignant skin tumors as a consequence of a persistent infection with Mastomys natalensis papillomavirus (MnPV). In this study, we mapped the MnPV transcriptome in productive lesions by both classical molecular techniques and high-throughput RNA sequencing. Combination of these methods revealed a complex and comprehensive transcription map, with novel splicing events not described in other papillomaviruses. Furthermore, these splicing occurrences could potentially lead to the expression of novel E2, E1^E4, E7 and L2 isoforms. Expression level estimation of each transcript showed that lateregion mRNAs considerably outnumber early transcripts, with species coding for L1 and E1^E4 being the most abundant. In summary, the full transcription map assembled in this study will allow us to further understand MnPV gene expression and the mechanisms that lead to natural tumour development.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Mastomys natalensis papillomavirus in productive lesions after natural infection

Salvermoser

Chotewutmontri

Braspenning-Wesch

et al. 2016

Journal of General Virology

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Persistent Immune Responses Induced by a Human Immunodeficiency Virus DNA Vaccine Delivered in Association with Electroporation in the Skin of Nonhuman Primates

et al. 2009

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Strategies to improve vaccine efficacy are still required, especially in the case of chronic infections, including human immunodeficiency virus (HIV). DNA vaccines have potential advantages over conventional vaccines; however, low immunological efficacy has been demonstrated in many experiments involving large animals and in clinical trials. To improve the immunogenicity of DNA vaccines, we have designed a plasmid vector exploiting the binding capacity of the bovine papillomavirus E2 protein and we have used electroporation (EP) to increase DNA uptake after intradermal inoculation. We demonstrated, in nonhuman primates (NHPs), efficient induction of anti-HIV immunity with an improved DNA vaccine vector encoding an artificial fusion protein, consisting of several proteins and selected epitopes from HIV-1. We show that a DNA vaccine delivery method combining intradermal injection and noninvasive EP dramatically increased expression of the vaccine antigen selectively in the epidermis, and our observations strongly suggest the involvement of Langerhans cells in the strength and quality of the anti-HIV immune response. Although the humoral responses to the vaccine were transient, the cellular responses were exceptionally robust and persisted, at high levels, more than 2 years after the last vaccine boost. The immune responses were characterized by the induction of significant proportions of T cells producing both interferon-gamma and interleukin-2 cytokines, in both subpopulations, CD4(+) and CD8(+). This strategy is an attractive approach for vaccination in humans because of its high efficacy and the possible use of newly developed devices for EP.

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Tyrphostin AG 555 Inhibits Bovine Papillomavirus Transcription by Changing the Ratio between E2 Transactivator/Repressor Function

Cited by 2 publications

References 67 publications

Transcriptome analysis of Mastomys natalensis papillomavirus in productive lesions after natural infection

Transcriptome analysis of Mastomys natalensis papillomavirus in productive lesions after natural infection

Persistent Immune Responses Induced by a Human Immunodeficiency Virus DNA Vaccine Delivered in Association with Electroporation in the Skin of Nonhuman Primates

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