Tyrphostins reduce chemotherapy-induced intestinal injury in mice: assessment by a biochemical assay

Abstract

Intestinal injury that results from chemotherapy belongs to the major factors of dose-limitation in tumour therapy. The tyrphostins AG1714 and AG1801 reduce cisplatin and 5-FU-induced small intestinal mucosal damage, using a quantitative biochemical assay. The assay is based on the determination of the enzymatic activity of gamma-glutamyl transpeptidase, a marker of the brush border epithelium of the small intestine.

Dairy milk fat augments paclitaxel therapy to suppress tumour metastasis in mice, and protects against the side-effects of chemotherapy

“…Apoptotic cells in 10 randomly selected crypts were counted and expressed as apoptotic bodies per crypt. The activity of jejunal c-glutamyl transpeptidase (c-GGT) was recorded as a separate measure of gut damage, as described previously [37]. Briefly, a segment of intestine was isolated and flushed with PBS, cut into pieces, placed into Tris-Triton buffer, and stirred for 2 min.…”

Oxymatrine attenuates intestinal ischemia/reperfusion injury in rats

“…13 Briefl y, a segment of intestine was isolated and fl ushed with phosphate-buffered saline, cut into pieces, placed in Tris-Triton buffer, and stirred for 2 min. The tissues were incubated on ice for 30 min, followed by centrifugation to collect the supernatants.…”

Taurine Attenuates Multiple Organ Injury Induced by Intestinal Ischemia Reperfusion in Rats

“…The methodology to measure ␥-GGT has been described previously [24]. Briefly, a segment of intestine was isolated and flushed with phosphate-buffered saline, cut into pieces, placed into TrisTriton buffer, and stirred for 2 min.…”