U-DCS: characterization of the first permanent human dendritic sarcoma cell line

Abstract

A dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-year-old Caucasian male patient. Since its establishment, U-DCS has maintained stable phenotypic characteristics in vitro and has a doubling time of approximately 2 days under standard culture conditions. U-DCS is growing with typical dendritic cell morphology in tissue and expresses the dendritic cell sarcoma immunophenotypic markers S100 protein, MHCI, MHCII, and vimentin. Expression analysis revealed transcripts for the toll-like receptors TLR3, -4, -9 and DDX58 (RIG-I), but not for TLR2. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. Karyotype-, CGH-, and mFISH analysis point to a chromosomal instability and a hypotetraploid karyotype with approximately 130 chromosomes. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors.

Parahydrogen-Polarized Fumarate for Preclinical in Vivo Metabolic Magnetic Resonance Imaging

“…The results obtained for the dendritic cell line (for the origin of U-DCS cells, see ref 23) are exemplarily shown in Figure 3. On treating these cells with the unpurified solution, the cells rounded up and aggregates of the contaminants were visible in phase-contrast micrographs (Figure 3a).…”

Niclosamide, but not ivermectin, inhibits anoctamin 1 and 6 and attenuates inflammation of the respiratory tract

“…In the present study, we show that Niclo (but not Iver) inhibits cytokine release by airway epithelial cells through inhibition of ANO1 and ANO6, and lowering cytoplasmic Ca 2+ concentrations, which is likely to suppress lung inflammation. However, as these drugs may also inhibit the release of cytokines by immunologically active cells, we examined the effects of Niclo and Iver in THP-1 macrophages [ 24 , 79 ], Jurkat T-cells [ 2 ], and U-DCS dendritic cells [ 54 ]. These cells express high levels of ANO6 but no ANO1 ( https://www.proteinatlas.org (Fig.…”

“…Jurkat T-cells were cultured in RPMI1640 supplemented with 10% FBS, 2 mM L -glutamine, and 1% penicillin/streptomycin. U-DCS dendritic cells were kindly provided by Prof. Dr. Kevin Mellert and Prof. Dr. Peter Möller (lnstitute of Pathology, Albert-Einstein-AIlee 23, D-89070 Ulm, Germany) [ 54 ]. Cells were grown in Iscove/RPMI medium supplemented with 10% fetal calf serum, 2 mM L -glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, and insulin–transferrin–sodium selenite supplement (ITS, Roche, Mannheim, Germany).…”