U‐V‐fluorescence studies on the <i>in vitro</i> intracellular accumulation of carcinogenic hydrocarbon

Richter, K; Saini, Virender K.

doi:10.1002/aja.1001070302

Cited by 10 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Why the cells should recover and return to a normal morphologic state when PAH is still present in the medium is not clear. Richter and Saini [1960] reported that no morphologic alterations could be observed in cells treated with B(a)P, but their concentration (1 .O-1.5 pg/ml) was considerably below that which we used, and the B(a)P was already in crystalline form rather than dissolved in a solvent. A later study by Allison and Malluci [1964] reported that PAH was selectively taken up and concentrated in lysosomes, where it remained for several weeks.…”

Section: Discussioncontrasting

confidence: 60%

Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro

Kocan¹,

Y.²,

Eriksen³

et al. 1983

Environ. mutagen

View full text Add to dashboard Cite

Vertebrate fibroblasts grown in vitro and exposed to various concentrations of the mutagen/carcinogen benzo(a)pyrene (B(a)P) internalized the compound and recrystallized it in lysosomes by 6-18 h postexposure. This phenomenon occurred when B(a)P at or above 10 micrograms/ml was introduced to the culture medium in a solvent such as DMSO or acetone but not when introduced dissolved in serum. Likewise, high fetal bovine serum concentrations in the culture medium (greater than 20%) as well as human serum (10%) inhibited crystal formation, presumably owing to lipid competition for the compound. Electron-microscopic observations of the cells during the uptake and crystal forming periods revealed that the cell membrane became altered within 3 h of exposure to B(a)P. This was followed by a return to normal of the membrane and the appearance of vesicles within the cell by 6-8 h. The vesicles then became filled with crystals which continued to grow while in the presence of B(a)P and disappeared when the cells were exposed to B(a)P-free culture medium or serum lipids. Introduction of B(a)P into culture medium containing delipidated serum resulted in crystal formation indistinguishable from that which occurred when whole serum was present. Crystals were not formed when total lipoprotein and the lipoprotein components VLDL, LDL, HDL2, and HDL3 were used as solvents for B(a)P. The process of crystal formation was inhibited by the addition of 10(-3) M KCN, and removal of the crystals was dependent on the concentration of lipoprotein in the culture medium.

show abstract

Section: Discussioncontrasting

confidence: 60%

Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro

Kocan¹,

Y.²,

Eriksen³

et al. 1983

Environ. mutagen

View full text Add to dashboard Cite

show abstract

“…Appearance of fluorescent intracytoplasmic bodies ( Figure 2 ) is a well-known response of cells and tissues to exposure to individual PAHs. Tissue fluorescence after injection of colloidal suspensions of BaP ( Peacock 1940 ) and intracellular fluorescence localization of hydrocarbons, including BaP ( Richter and Saini 1960 ), have long been recognized. In a later study, BaP solubilized in serum was taken up by HeLa and monkey kidney cells in culture ( Allison and Mallucci 1964 ).…”

Section: Discussionmentioning

confidence: 99%

Combustion-Derived Ultrafine Particles Transport Organic Toxicants to Target Respiratory Cells

Penn

Murphy

Barker

et al. 2005

Environ Health Perspect

View full text Add to dashboard Cite

Epidemiologic evidence supports associations between inhalation of fine and ultrafine ambient particulate matter [aerodynamic diameter ≤ 2.5 μm (PM2.5)] and increases in cardiovascular/respiratory morbidity and mortality. Less attention has been paid to how the physical and chemical characteristics of these particles may influence their interactions with target cells. Butadiene soot (BDS), produced during combustion of the high-volume petrochemical 1,3-butadiene, is rich in polynuclear aromatic hydrocarbons (PAHs), including known carcinogens. We conducted experiments to characterize BDS with respect to particle size distribution, assembly, PAH composition, elemental content, and interaction with respiratory epithelial cells. Freshly generated, intact BDS is primarily (> 90%) PAH-rich, metals-poor (nickel, chromium, and vanadium concentrations all < 1 ppm) PM2.5, composed of uniformly sized, solid spheres (30–50 nm) in aggregated form. Cells of a human bronchial epithelial cell line (BEAS-2B) exhibit sequential fluorescent responses—a relatively rapid (~ 30 min), bright but diffuse fluorescence followed by the slower (2–4 hr) appearance of punctate cytoplasmic fluorescence—after BDS is added to medium overlying the cells. The fluorescence is associated with PAH localization in the cells. The ultrafine BDS particles move down through the medium to the cell membrane. Fluorescent PAHs are transferred from the particle surface to the cell membrane, cross the membrane into the cytosol, and appear to accumulate in lipid vesicles. There is no evidence that BDS particles pass into the cells. The results demonstrate that uptake of airborne ultrafine particles by target cells is not necessary for transfer of toxicants from the particles to the cells.

show abstract

“…Fluorescence microscopy has hitherto failed to de monstrate the presence of fluorescent carcinogens in cell nuclei after in vivo administration [1,2], This is in contradiction with the results obtained from auto radiography [3,4], subcellular fractionation and isola tion of carcinogens bound to DNA from the tissues exposed in vivo or in vitro [5][6][7], These studies have been reviewed recently [8],…”

Section: Introductionmentioning

confidence: 52%

Cellular Localization of Chemical Carcinogens Studied by Fluorescence Microscopy

Stora¹

1980

Oncology

View full text Add to dashboard Cite

show abstract

U‐V‐fluorescence studies on the in vitro intracellular accumulation of carcinogenic hydrocarbon

Cited by 10 publications

References 28 publications

Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro

Sequestration and release of polycyclic aromatic hydrocarbons by vertebrate cells in vitro

Combustion-Derived Ultrafine Particles Transport Organic Toxicants to Target Respiratory Cells

Cellular Localization of Chemical Carcinogens Studied by Fluorescence Microscopy

Contact Info

Product

Resources

About