A number of ethoxylated nonionic surfactants differing in hydrophobe branching and chainlengths have been evaluated for environmental responses. Screening biodegradation tests show that those nonionics having more than one methyl group per hydrophobe degrade considerably slower than those having less extensive branching. Continuous flow-through activated sludge tests, simulating actual waste treatment, show that the more highly branched nonionics biodegrade more slowly and less extensively than those with less hydrophobe branching. In addition, treated effluents originating from influents containing the more highly branched nonionics tend to be more surface active and more toxic to aquatic species than those originating from influents containing surfactants with less hydrophobe branching. Under conditions simulating plant stress, such as high surfactant concentrations in the influent or low temperature, biodegradation of the highly branched nonionics was considerably less extensive, while biodegradation of the linear nonionics was not affected to any measurable degree compared to more normal operating conditions. KEY WORDS: Activated sludge, alcohol ethoxylates, aquatic toxicity, linear and branched hydrophobes, nonylphenol ethoxylates, primary and ultimate biodegradation.