Über den Mechanismus des negativen Pasteur-Effekts bei Hefen der Gattung Saccharomyces

Idziak, Edmund S.; Wikén, T.

doi:10.1159/000161324

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Generally yeast cell pH values are obtained with a glass electrode in the cell suspension after freezing and thawing the cells once (CONWAY & DOWNEY 1950;SUOMALAINEN &OURA 1955 andIDZIAK &WIKEN 1962). This procedure leads to lower pH values than when the cells are boiled before determining the pH (CONWAY, BRADY & CARTON 1950;BRANDT 1945).…”

Section: Introductionmentioning

confidence: 99%

DETERMINATION OF THE YEAST CELL pH

Borst-Pauwels¹,

Dobbelmann²

1972

Acta Botanica Neerlandica

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Once freezing and thawing the yeast leads to incomplete destruction of the cell membrane and to too low values of the cell pH determined in the cell suspension with the glass electrode. The cells are rendered completely permeable through boiling, treatment with acetone, or freezing and thawing the cell repeatedly. Cell pH's obtained in this ways are definitely higher. The differences in cell pH's observed with completely broken cells and partly broken cells can be accounted for quantitatively to the presence of intact cells in the only once frozen and thawed cell suspension.

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Section: Introductionmentioning

confidence: 99%

DETERMINATION OF THE YEAST CELL pH

Borst-Pauwels¹,

Dobbelmann²

1972

Acta Botanica Neerlandica

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“…This phenomenon was dif ferent from the Crabtree effect10) or negative Pasteur effect. 11) As reported previously,2) the result of in vestigation on intracellular glucose contents suggested that inositol-deficiency caused low glycolytic activity itself. This paper presents the Preliminary experiments showed that within 5 min 14CO2 from 1-14C-glucose or 6-14C-glucose increased linearly with course of time.…”

mentioning

confidence: 55%

Biochemical studies on myo-inositol in microbes. VII. Effect of inositol-deficiency on glycolysis of the inositolexacting yeast Schizosaccharomyces pombe.

Dohi

Arima

1980

Agricultural and Biological Chemistry

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In Schizosaccharomyces pombe, inositol-deficiency caused a decrease in Crabtree effect and an enhanced Pasteur effect. A time-course estimation of intracellular glucose , glycolytic intermediates, ATP and other components showed that decreased glycolytic activity possibly occurred before low glucose uptake activity and that low transport activity could hardly have caused of low glycolytic activity. The activities of all glycolytic enzymes tested seemed to be low in vivo , as well as the activities of enzymes in the hexose mono-phosphate shunt. These results suggest that inositol deficiency caused pleiotropic situation, possibly due to structural abnormality. The possible cause of low aerobic fermentation activity may be due to the difference in Km values between pyruvate dehydrogenase complex and pyruvate decarboxylase. An oxidative phosphorylation-uncoupling mutant was isolated and was used in these investigations .

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Über den Mechanismus des negativen Pasteur-Effekts bei Hefen der Gattung Saccharomyces

Cited by 2 publications

References 3 publications

DETERMINATION OF THE YEAST CELL pH

DETERMINATION OF THE YEAST CELL pH

Biochemical studies on myo-inositol in microbes. VII. Effect of inositol-deficiency on glycolysis of the inositolexacting yeast Schizosaccharomyces pombe.

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