Über den Stoffwechsel der Hefe nach dem Einfrieren in flüssiger Luft

The 1964 Nobel Prize for the discovery of the biosynthesis of cholesterol

“…When investigating the metabolism of tri‐deuterated acetate by yeast, they found that formed citrate contained the expected amount of the isotope whereas succinate contained only half of the expected amount. On the basis of the findings that yeast cells form succinate from acetate and that deuterium was mainly recovered in fatty acids and sterols with only minor amounts in carbohydrates, Lynen proposed that fatty acids and sterols are directly formed from acetate (8). In the meantime, Wieland found that yeast cells that are shaken with oxygen will lack substrate for cellular respiration and need some incubation time before they are able to oxidize acetic acid.…”