Über die Eignung von Transplantationsbedingungen des AMel-3-Hamster-Melanoms zur Beurteilung von Einflüssen auf das Tumorwachstum

Abstract

To obtain optimal test conditions the growth velocities of the amelanotic hamster melanoma, AMel 3 Fortner, are compared with the transplantated cell numbers. The tumor is transplantated almost quantitatively as a definite cell suspension and weighed after constant time. Within 14 days, alterations of growth velocity are both most evident and least errant at about 10⁶ transplantated cells.

Operability of Fortner’s Hamster Melanoma AMel 3

“…They received pellets recipe R, forced wheat, and water ad libitum. At the time of the experiment, the animals were 4-6 months old and had an average weight of 100 g. Defined tumour cell suspensions (0.5 ml), each containing 2 x 10° living AMel 3 melanoma cells1 obtained by carefully-fractionated trypsination, were subcutaneously transplanted into the right side of the body [Wohlrab and Peker, 1971] in a total of 31 animals. 4-7 days after transplantation, the animals were pre-treated intramuscularly with 0.02 mg atropine (Atropinum sulfuricum, Chimpharm, Sofia) and 2 mg promazine (Sinophenin, Deutsches Hydrierwerk, Rodleben), and then anaesthesized intraperitoneally with 150 mg urethan (Athylurethan, Philopharm, Quedlinburg) in slight ether anaesthesia.…”

“…In the present studies, the therapeutic effectiveness of extensive surgical treatm ent has been examined, choosing experimental conditions under which the influences on the tum our growth appear most strikingly [Wohlrab and Peker, 1971],…”

Combined Surgical and Chemotherapeutic Treatment of Fortner's Amelanotic Hamster Melanoma Amel 3

“…As recently doseribed in detail elsewhere (Wohlrab and Peker, 1971;Haneke et al, in press), the animals were injected with 2 x U)^ viable AMel 3 cells subcutaneously into the right side of the body. As recently doseribed in detail elsewhere (Wohlrab and Peker, 1971;Haneke et al, in press), the animals were injected with 2 x U)^ viable AMel 3 cells subcutaneously into the right side of the body.…”

“…Thirty strain Z ^ golden hamsters of both sexes, aged 4 to 6 months and having an average weight of 100 g were used. As recently doseribed in detail elsewhere (Wohlrab and Peker, 1971;Haneke et al, in press), the animals were injected with 2 x U)^ viable AMel 3 cells subcutaneously into the right side of the body. Four days after inoculation, the tumours (mostly larger than peas) of 15 animals were removed by radical surgery (Table I).…”