Uber Sichtbarmachung und Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf Goldrubingläser

Siedentopf, H.; Zsigmondy, Richard

doi:10.1002/andp.19023150102

Cited by 307 publications

(240 citation statements)

References 7 publications

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“…The optical properties of colloidal gold have been studied for over 100 years with major contributions by Zsigmondy 10 and Mie 11 . The discovery of surface enhanced Raman scattering (SERS) of molecules near metal structures has renewed the interest in plasmon resonances of metal particles 12 .…”

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confidence: 99%

A molecular ruler based on plasmon coupling of single gold and silver nanoparticles

et al. 2005

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biomolecules with up to zeptomole sensitivity in bulk experiments 7,8,13 . Moreover, the binding of proteins to single functionalized particles can be followed by dark-field microscopy, by exploiting the dependence of the plasmon resonance wavelength on the refractive index of the particle's surroundings 14,15 . The plasmon resonance wavelength of a metal nanoparticle is also affected by other nanoparticles that are in its immediate environment. When two nanoparticles are brought into proximity, their plasmons couple, which shifts the resonance wavelength depending on the particle separation. Since this effect is well known theoretically 16, 17 and experimentally observed for fixed distances 18, 19 , we sought to explore its use as molecular ruler.We applied this 'plasmon ruler' to study the dynamics of DNA hybridization on a single We employed 40 nm diameter gold and silver nanoparticles. The particle diameter was chosen to ensure a sufficiently intense light scattering signal while minimizing any effects of the particles on proximal biomolecules. However, we subsequently found that a reduction in particle size to 20 nm for silver and 30 nm for gold is possible with the current technique.Particles were illuminated with unpolarized white light and light scattered by individual particles was collected by a darkfield microscope in transmission mode (Fig. 1a) 25 . Upon introduction of streptavidin-functionalized particles into the BSA-biotin coated glass chamber, we immediately observed numerous scattering sources adhering to the chamber surfaces. Nanoparticles were vividly colored: individual silver nanoparticles were blue (Fig. 1b), gold nanoparticles were green ( Fig. 1c), aggregates were red-shifted compared to individual particles (typically by about 50 nm for gold, 150nm for silver), and dust and scratches were white.Our first application of plasmon coupling was to monitor the directed assembly of functionalized particle pairs. We used the surface immobilized particles (Figs. 1b and c) as anchors for single stranded DNA (ssDNA) functionalized particles. The 33-nucleotide ssDNA molecules had a biotin at their 3' end, allowing them to bind to the streptavidin coated anchor particles (Fig. 1a). Shortly after introducing the ssDNA-functionalized particles into the chamber, some scattering centers suddenly changed color due to dimer formation. Silver particles turned from blue to green (Fig. 1b), gold particles turned from green to orange (Fig. 1c). The spectral shift upon dimer formation is considerably larger for the silver particles (102 nm) than for gold particles (23 nm, Fig. 1d). The fraction of surface immobilized particles that captured a ssDNAfunctionalized particle ranged from 10% to 86 % depending on the time the samples had been stored, with fresher particles performing better. Aggregates of more than two particles were easily identified by their intensity and distinct color with multiple peaks in the spectrum (see for example the purple dot in Fig 1b). To avoid these aggregates of more than two partic...

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A molecular ruler based on plasmon coupling of single gold and silver nanoparticles

et al. 2005

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“…Confocal achieves optical sectioning by the use of a pinhole at the detection focal plane to reject out-of-focus light, whereas two-photon utilizes the fact that only simultaneous absorption of two photons results in fluorescence emission, an event much more likely to occur at the point of highest light intensity in the sample (the focal plane). Light sheet microscopy, in contrast, builds upon a 100-year-old idea to illuminate the sample from the side with a thin sheet of light and detect the emitted fluorescence signal with an in-focus orthogonally arranged objective (Siedentopf and Zsigmondy 1903;Huisken and Stainier 2009). The optical sectioning is achieved by the confinement of illumination to a selective plane, which allows use of fast CCD or sCMOS cameras to capture the whole image simultaneously, and results in an increase of 2-3 orders of magnitude in imaging speed compared to confocal and two-photon microscopy.…”

Section: Clarity-optimized Light Sheet Microscopymentioning

confidence: 99%

Form Meets Function in the Brain: Observing the Activity and Structure of Specific Neural Connections

Deisseroth

2016

Research and Perspectives in Neurosciences

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Recent advances in neuroscience have enabled increasingly detailed insight into the activity and structure of brain circuitry. In previous work, we have developed and applied methods for precisely controlling the activity of specific cells and projections within neural systems during behavior (optogenetics). Here I review distinct complementary technological approaches for observing natural activity patterns in these cells and projections during behavior (fiber photometry) and for obtaining anatomical insights into the wiring and molecular phenotype of these circuit elements within the intact mammalian brain (CLARITY-optimized lightsheet microscopy). Together these approaches may help further advance understanding of the circuit dynamics and wiring patterns that underlie adaptive and maladaptive behavior.

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“…Light sheet microscopy or ultramicroscopy, originally developed in 1903 [41], reappeared in 1983 when it was applied to fixed and cleared tissue as orthogonal-plane fluorescence optical sectioning (OPFOS) [42], and as thin laser sheet imaging microscopy (TSLIM) in 2002 [43] finally being applied to whole fixed and cleared organs as ultramicroscopy [44]. However, it was not until Huisken et al in 2004 [45] presented selective plane illumination microscopy (SPIM) data applied to in vivo zebrafish imaging that light sheet microscopy really took off.…”

Section: Light Sheet Microscopymentioning

confidence: 99%

Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll

Koberstein-Schwarz

Ntziachristos

2015

Trends in Biotechnology

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Uber Sichtbarmachung und Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf Goldrubingläser

Cited by 307 publications

References 7 publications

A molecular ruler based on plasmon coupling of single gold and silver nanoparticles

A molecular ruler based on plasmon coupling of single gold and silver nanoparticles

Form Meets Function in the Brain: Observing the Activity and Structure of Specific Neural Connections

Unleashing Optics and Optoacoustics for Developmental Biology

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