UbiNAAT: a multiplexed point-of-care nucleic acid diagnostic platform for rapid at-home pathogen detection

Abstract

The COVID-19 pandemic increased demands for respiratory disease testing to facilitate treatment and limit transmission, demonstrating in the process that most existing test options were too complex and expensive to...

Point-of-Care Multiplex Detection of Respiratory Viruses

“…15 As an example of RT-LAMP-based POC platform, Lucira Health has created a system for multiplex respiratory testing that can simultaneously detect SARS-CoV-2, IAV, and IBV. 16 However, the RT-LAMP technique has certain limitations for multiplexed detection, because it uses 4−6 primers per individual assay. The use of a large number of primers in multiplexed assays increases the likelihood of amplifying nonspecific targets.…”

“…Due to its isothermal nature, RT-LAMP does not require a thermocycler, and the Bst polymerase, used in this reaction, possesses unique characteristics, including robustness to relatively high levels of amplification inhibitors, enabling the possibility of avoiding the use of RNA extraction/purification kits . As an example of RT-LAMP-based POC platform, Lucira Health has created a system for multiplex respiratory testing that can simultaneously detect SARS-CoV-2, IAV, and IBV . However, the RT-LAMP technique has certain limitations for multiplexed detection, because it uses 4–6 primers per individual assay.…”