Ubiquinol decreases hemorrhagic shock/resuscitation-induced microvascular inflammation in rat mesenteric microcirculation

Abstract

Hemorrhagic shock (HS) is a leading cause of death in traumatic injury. Ischemia and hypoxia in HS and fluid resuscitation (FR) creates a condition that facilitates excessive generation of reactive oxygen species (ROS). This is a major factor causing increased leukocyte‐endothelial cell adhesive interactions and inflammation in the microcirculation resulting in reperfusion tissue injury. The aim of this study was to determine if ubiquinol (coenzyme Q10) decreases microvascular inflammation following HS and FR. Intravital microscopy was used to measure leukocyte‐endothelial cell adhesive interactions in the rat mesentery following 1‐h of HS and 2‐h post FR with or without ubiquinol. Hemorrhagic shock was induced by removing ~ 40% of anesthetized Sprague Dawley rats' blood volume to maintain a mean arterial blood pressure <50 mmHg for 1 h. Ubiquinol (1 mg/100 g body weight) was infused intravascularly in the ubiquinol group immediately after 1‐h HS. The FR protocol included replacement of the shed blood and Lactate Ringer's in both the control and ubiquinol groups. We found that leukocyte adherence (2.3 ± 2.0), mast cell degranulation (1.02 ± 0.01), and ROS levels (159 ± 35%) in the ubiquinol group were significantly reduced compared to the control group (10.8 ± 2.3, 1.36 ± 0.03, and 343 ± 47%, respectively). In addition, vascular permeability in the control group (0.54 ± 0.11) was significantly greater than the ubiquinol group (0.34 ± 0.04). In conclusion, ubiquinol attenuates HS and FR‐induced microvascular inflammation. These results suggest that ubiquinol provides protection to mesenteric microcirculation through its antioxidant properties.

Coenzyme Q10 in Cardiovascular and Metabolic Diseases: Current State of the Problem

“…Prospective observational study of post-arrest patients demonstrated that COQ10 levels could be named a statistically significant predictor of poor neurologic outcome and in-hospital mortality [150]. These results are similar to other studies, which demonstrated its role in septic and hemorrhagic shocks [151-153]. This underlines the importance of metabolic resuscitation particularly in case of septic shock but may be useful also in other conditions with severely altered hemodynamics [154].…”

Ubiquinol treatment for TBI in male rats: Effects on mitochondrial integrity, injury severity, and neurometabolism

“…Ubiquinol (reduced form of CoQ10) has been used to enhance mitochondrial energy metabolism in many diseases such as heart failure (Kawashima, ), hemorrhagic shock (Shen et al, ), and amyotrophic lateral sclerosis (Csilla, ). Kalayci et al examined the protective effect of CoQ10 on neuronal damage in rats after administering a moderate TBI.…”

Resveratrol Improves Survival and Prolongs Life Following Hemorrhagic Shock

“…Therefore the effect of resveratrol as an antioxidant is very pertinent in this model. In addition to resveratrol and tempol, several other agents that are known to reduce oxidative stress have also been tested in hemorrhagic shock/resuscitation models, in shorter term studies (24,44,45). In various models of oxidant stress, it has been reported that resveratrol inhibits the expression and activity of NADPH oxidase, enhances activity of several antioxidant enzymes, including that of the SOD and scavenges reactive oxygen free radicals (ROS) (46).…”