Ubiquinol preserves immune cells in gamma-irradiated rats: Role of autophagy and apoptosis in splenic tissue

Elkenawy, Nora Mohamed; Ghaiad, Heba Ramadan; Ibrahim, Sherehan Mohamed; Aziz, Ramy Karam; Rashad, Eman; Eraqi, Walaa Ahmed

doi:10.1016/j.intimp.2023.110647

Cited by 3 publications

(1 citation statement)

References 71 publications

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“…51 Nrf2 upregulation, as in the sulbutiamine-treated group, can increase antiapoptotic protein, Bcl-2, expression with subsequent protection from the apoptotic cell death via binding of Nrf2 to the Bcl-2 gene antioxidant response element, thus control Bcl-2. 54,55 This is supported by the positive correlation between Nrf2 and Bcl-2 in the present study. A recent study also demonstrated that the protective effect of carvedilol against aluminuminduced testicular toxicity was mediated via upregulation of the antiapoptotic proteins Nrf 2 and Bcl 2 which suppressed testicular tissue apoptosis in rats.…”

Section: Discussionsupporting

confidence: 85%

Ameliorating diabetes‐induced testicular dysfunction by modulating PKC/Nrf2/Bcl‐2 signaling: Protective role of sulbutiamine

Abdelmonem,

Ali,

Al‐Mokaddem

et al. 2024

BioFactors

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The prevalence of testicular dysfunction is increasing as it is a common diabetes mellites (DM) complication. The objective of this study is to explore the potential protective effect of sulbutiamine against testicular hypofunction associated with streptozotocin (STZ)‐induced DM in rats. Sulbutiamine was administered orally (60 mg/kg) to male Wistar rats for 8 weeks starting 72 h after a single injection of STZ (45 mg/kg, i.p.). Blood glucose level (BGL), serum testosterone level, sperm number, and motility were determined. Testicular tissue was examined histopathologically, and the Johnson score was evaluated. Levels of malondialdehyde (MDA), protein kinase C (PKC), nuclear factor erythroid‐derived 2‐like 2 (Nrf2), and proliferating cell nuclear antigen (PCNA) were measured. Apoptosis was evaluated by immunohistochemical determination of B‐cell lymphoma protein 2 (Bcl‐2), Bcl‐2 associated X‐protein (Bax), and caspase‐3. Sulbutiamine administration managed to reduce BGL and boost testicular function as manifested by increased testicular weight, testosterone level, sperm number, and motility compared to the STZ group. Additionally, histopathological examination revealed an improved histological picture and Johnson score of testicular tissue after sulbutiamine treatment. Sulbutiamine administration reduced testicular PKC, MDA, and PCNA levels and increased Nrf2 compared to the untreated group. Moreover, sulbutiamine treatment suppressed apoptosis triggered by STZ as evidenced by elevated Bcl‐2, decreased Bax and reduced caspase‐3. The present work revealed for the first time a promising protective role of sulbutiamine against STZ‐induced testicular dysfunction which may add to the clinical utility of sulbutiamine. The underlying mechanisms involve reducing BGL and PKC, activating Nrf2 and inhibiting apoptosis.

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Section: Discussionsupporting

confidence: 85%

Ameliorating diabetes‐induced testicular dysfunction by modulating PKC/Nrf2/Bcl‐2 signaling: Protective role of sulbutiamine

Abdelmonem,

Ali,

Al‐Mokaddem

et al. 2024

BioFactors

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Novel quinazoline sulfonamide-based scaffolds modulate methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in immunodeficient irradiated model: Regulatory role of TGF-β

Soliman,

Ghorab,

Ghorab

et al. 2024

Bioorganic Chemistry

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Gastroprotective and microbiome-modulating effects of ubiquinol in rats with radiation-induced enteropathy

Eraqi,

El-Sabbagh,

Aziz

et al. 2024

anim microbiome

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Radiation enteritis is a frequently encountered issue for patients receiving radiotherapy and has a significant impact on cancer patients' quality of life. The gut microbiota plays a pivotal role in intestinal function, yet the impact of irradiation on gut microorganisms is not fully understood. This study explores the gastroprotective effect and gut microbiome-modulating potential of ubiquinol (Ubq), the reduced form of the powerful antioxidant CoQ-10. For this purpose, male albino rats were randomly assigned to four groups: Control, IRR (acute 7 Gy γ-radiation), Ubq_Post (Ubq for 7 days post-irradiation), and Ubq_Pre/Post (Ubq for 7 days pre and 7 days post-irradiation). The fecal microbiomes of all groups were profiled by 16S rRNA amplicon sequencing followed by bioinformatics and statistical analysis. Histopathological examination of intestinal tissue indicated severe damage in the irradiated group, which was mitigated by ubiquinol with enhanced regeneration, goblet cells, and intestinal alkaline phosphatase expression. Compared to the irradiated group, the Ubq-treated groups had a significant recovery of intestinal interleukin-1β, caspase-3, nitric oxide metabolites, and thio-barbituric reactive substances to near-healthy levels. Ubq_Pre/Post group displayed elevated peroxisome proliferator-activated receptor (PPAR-γ) level, suggesting heightened benefits. Serum insulin reduction in irradiated rats improved post-Ubq treatment, with a possible anti-inflammatory effect on the pancreatic tissue. Fecal microbiota profiling revealed a dysbiosis state with a reduction of bacterial diversity post-irradiation, which was re-modulated in the Ubq treated groups to profiles that are indistinguishable from the control group. These findings underscore Ubq's gastroprotective effects against radiation-induced enteritis and its potential in restoring the gut microbiota’s diversity and balance.

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Ubiquinol preserves immune cells in gamma-irradiated rats: Role of autophagy and apoptosis in splenic tissue

Cited by 3 publications

References 71 publications

Ameliorating diabetes‐induced testicular dysfunction by modulating PKC/Nrf2/Bcl‐2 signaling: Protective role of sulbutiamine

Ameliorating diabetes‐induced testicular dysfunction by modulating PKC/Nrf2/Bcl‐2 signaling: Protective role of sulbutiamine

Novel quinazoline sulfonamide-based scaffolds modulate methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in immunodeficient irradiated model: Regulatory role of TGF-β

Gastroprotective and microbiome-modulating effects of ubiquinol in rats with radiation-induced enteropathy

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