Ubiquinone accumulates in the mitochondria of yeast mutated in the ubiquinone binding protein, Qcr8p

Hagerman, Ruth A.; Waring, Natashya J.; Willis, Richard A.; Hagerman, Ann

doi:10.1016/j.bbrc.2006.03.110

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…In addition, CYC1-cytochrome c isoform 1 related to the electron carrier of the mitochondrial inner membrane (39), and AFG1, which may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase (59) subunits were also reduced in response to the high inhibitory concentration of NP for 180 min. This finding is consistent with our previous study on BPA toxicity (10), and several studies have shown that mutations and defects in various subunits downregulated in the present study may severely or completely inhibit the complex activity (16,25,48,63) and may cause various neuromuscular disorders in humans (62). Moreover, the human homolog of the CYC1 gene has been associated with insulin-responsive hyperglycemia (39).…”

Section: Resultssupporting

confidence: 93%

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Bereketoğlu

Arğa

Eraslan

et al. 2017

Physiological Genomics

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Bioaccumulative environmental estrogen, nonylphenol (NP; 4-nonylphenol), is widely used as a nonionic surfactant and can affect human health. Since genomes of and higher eukaryotes share many structural and functional similarities, we investigated subcellular effects of NP on BY4742 cells by analyzing genome-wide transcriptional profiles. We examined effects of low (1 mg/l; <15% cell number reduction) and high (5 mg/l; >65% cell number reduction) inhibitory concentration exposures for 120 or 180 min. After 120 and 180 min of 1 mg/l NP exposure, 187 (63 downregulated, 124 upregulated) and 103 genes (56 downregulated, 47 upregulated), respectively, were differentially expressed. Similarly, 678 (168 repressed, 510 induced) and 688 genes (215 repressed, 473 induced) were differentially expressed in cells exposed to 5 mg/l NP for 120 and 180 min, respectively. Only 15 downregulated and 63 upregulated genes were common between low and high NP inhibitory concentration exposure for 120 min, whereas 16 downregulated and 31 upregulated genes were common after the 180-min exposure. Several processes/pathways were prominently affected by either low or high inhibitory concentration exposure, while certain processes were affected by both inhibitory concentrations, including ion transport, response to chemicals, transmembrane transport, cellular amino acids, and carbohydrate metabolism. While minimal expression changes were observed with low inhibitory concentration exposure, 5 mg/l NP treatment induced substantial expression changes in genes involved in oxidative phosphorylation, cell wall biogenesis, ribosomal biogenesis, and RNA processing, and encoding heat shock proteins and ubiquitin-conjugating enzymes. Collectively, these results provide considerable information on effects of NP at the molecular level.

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Section: Resultssupporting

confidence: 93%

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Bereketoğlu

Arğa

Eraslan

et al. 2017

Physiological Genomics

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“…Mutation of L63 and W104 abolished the ability of Coq10 to complement the coq10 deletion mutation, and lowered, but did not completely inhibit, the CoQ‐binding activity of Coq10. The components of the electron transfer system NADH‐ubiquinone reductase [29], succinate‐ubiquinone reductase [30] and ubiquinone‐cytochrome c reductase [31,32] have been reported to contain CoQ‐binding subunits. These are necessary for the electron transfer mediated by CoQ in each reaction.…”

Section: Discussionmentioning

confidence: 99%

Coq10, a mitochondrial coenzyme Q binding protein, is required for proper respiration in Schizosaccharomyces pombe

Cui

Kawamukai

2009

The FEBS Journal

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It has been widely accepted that most coenzyme Q (CoQ) exists freely in the mitochondrial membrane as a CoQ pool. However, the recent identification of a mitochondrial CoQ‐binding protein, termed Coq10, in budding yeast has the potential to change our current view of CoQ status in membranes. Here, we studied the counterpart of budding yeast Coq10 (also termed Coq10) in fission yeast. Fission yeast coq10 null mutants exhibited a similar, but less severe, phenotype to CoQ‐deficient fission yeast, including the requirement for antioxidants for proper growth on minimal medium, increased sensitivity to H2O2, high levels of H2S production, and a deficiency in respiration. The coq10 null mutant produced nearly normal levels of CoQ10, suggesting that coq10 does not belong to the group of CoQ biosynthetic genes. To elucidate the role of Coq10, we expressed recombinant coq10 in Escherichia coli, and found that CoQ8 was present in purified recombinant Coq10. Mutational analysis of 13 conserved residues of Coq10 revealed that two hydrophobic amino acid residues, leucine 63 (L63) and tryptophan 104 (W104), play an important role in Coq10 binding to CoQ. An L63A/W104A double mutant of Coq10 exhibited lower CoQ‐binding activity than either of the single mutants, and was unable to complement the coq10 deletion in fission yeast. A human Coq10 ortholog was able to functionally compensate for the absence of coq10 in fission yeast, suggesting that Coq10 is important for proper respiration in a variety of organisms.

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Current awareness on yeast

2006

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 9th. Aug. 2006)

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Ubiquinone accumulates in the mitochondria of yeast mutated in the ubiquinone binding protein, Qcr8p

Cited by 5 publications

References 22 publications

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Coq10, a mitochondrial coenzyme Q binding protein, is required for proper respiration in Schizosaccharomyces pombe

Current awareness on yeast

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