Ubiquitin E3 Ligase A20 Facilitates Processing Microbial Product in Nasal Epithelial Cells

An, Yun-Fang; Li, Tong-Li; Geng, Xinyu; Yang, Gui; Zhao, Changqing; Yang, Ping‐Chang

doi:10.1074/jbc.m112.392639

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…Endocytosed antigens are wrapped by plasma membrane to form endosomes, and the latter can fuse with lysosomes; the antigens can then be decomposed by the enzymes in lysosomes. Our data are in line with Li et al () indicating that ubiquitin editing enzyme, A20, facilitates the fusion of endosomes and lysosomes, as observed by An et al (). We found >60% endosomes were fused with lysosomes in the naive states, which was markedly reduced by tryptase in culture.…”

Section: Discussionsupporting

confidence: 93%

Activation of protease‐activated receptor‐2 disrupts vaginal epithelial barrier function

Feng

Wei³

et al. 2014

Cell Biology International

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The epithelial cell barrier function is a critical factor in the maintenance of the homeostasis of the vaginal mucosa. This study elucidates one of the mast cell-derived chemical mediators, tryptase, on compromising the vaginal epithelial barrier function. The results showed that human vaginal cell line, VK2, express PAR2. Activation of PAR2 increases the expression of ADAM10 in VK2 cells, interferes with the endosome/lysosome fusion, and compromises the epithelial barrier function. We conclude that the activation of PAR2 on VK2 cells increases the expression of ADAM10, and compromises the VK2 monolayer barrier function.

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Section: Discussionsupporting

confidence: 93%

Activation of protease‐activated receptor‐2 disrupts vaginal epithelial barrier function

Feng

Wei³

et al. 2014

Cell Biology International

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“…21 Similar findings were reported by Li et al, in which Li et al found that deficiency of A20 promoted antigen transport across airway epithelial cells via a transcellular pathway. For example, An et al indicated that the fusion of endosome/lysosome is required in the degradation of microbial products in nasal epithelial cells; the disturbance of the expression of ubiquitin E3 ligase A20 interferes with such a transcellular epithelial barrier function.…”

Section: Discussionsupporting

confidence: 83%

“…For example, An et al indicated that the fusion of endosome/lysosome is required in the degradation of microbial products in nasal epithelial cells; the disturbance of the expression of ubiquitin E3 ligase A20 interferes with such a transcellular epithelial barrier function. 21 Similar findings were reported by Li et al, in which Li et al found that deficiency of A20 promoted antigen transport across airway epithelial cells via a transcellular pathway. 22 Our results are in line with those pioneer studies by showing that disturbance causes hyperpermeability of the corneal epithelial barrier.…”

Section: Discussionsupporting

confidence: 83%

Tryptase compromises corneal epithelial barrier function

Jie

Zhang

et al. 2013

Cell Biochemistry & Function

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Corneal epithelial barrier dysfunction is harmful to corneal health; the pathogenesis is unclear. This study aims to elucidate the mechanism by which tryptase compromises corneal epithelial barrier function. Human corneal epithelial cell line (HCE cells) was cultured into monolayers using as a study platform. Quantitative reverse transcription polymerase chain reaction and Western blotting were employed to detect the expression of matrix metalloprotenases (MMP)9. The endosome/lysosome fusion was observed by confocal microscopy. The corneal epithelial barrier function was assessed in Transwell system. The results showed that HCE cells expressed proteinase-activated receptor (PAR)2. Activation of PAR2 by tryptase induced expression of MMP9 in HCE cells, interfered with the fusion of endosome/lysosome, and compromised the epithelial barrier function, which could be prevented by pretreatment with MMP9 inhibitor. We conclude that tryptase can increase the expression of MMP9 in HCE cells and compromise the epithelial barrier function.

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“…The endocytic proteins can be degraded by the hydrolases in lysosomes (Watts, ). The fusion of endosome/lysosome is important in processing the endocytic proteins in the epithelial cells (An et al, ; Chen et al, ). The factors modulating the fusion of endosome/lysosome are not fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Integrin alpha v beta 6 contributes to maintaining corneal epithelial barrier function

Guo

Wang

et al. 2013

Cell Biology International

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The corneal epithelial barrier dysfunction is associated with a number of disorders of the cornea. This study aims to investigate the role of integrin alpha v beta 6 (avb6) in maintaining the corneal epithelial barrier function. In this study, the association of avb6 and endosome/lysosome in the human corneal epithelial cell line, HCE cells, was observed with immunocytochemistry. The corneal epithelial barrier function was assessed with HCE monolayers in Transwells. The results showed that avb6 was observed attaching to endosomes in HCE cells. Knockdown of avb6 resulted in disturbing the fusion of endosome/lysosome. Tumor necrosis factor (TNF)-α could bind avb6 to form a complex that interfered with the fusion of endosome/lysosome in HCE cells. With the HCE monolayer as a corneal epithelial barrier model, the knockdown of avb6 or exposure to TNF-α markedly increased the epithelial barrier permeability to a macomolecular protein, ovalbumin, in Transwell system. After passing through the deficient epithelial barrier, the ovalbumin molecules still conserved the antigenicity. We conclude that the integrin avb6 plays an important role in the fusion of endosome/lysosome in corneal epithelial cells; inhibition of avb6 results in corneal epithelial barrier dysfunction.

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Ubiquitin E3 Ligase A20 Facilitates Processing Microbial Product in Nasal Epithelial Cells

Cited by 12 publications

References 23 publications

Activation of protease‐activated receptor‐2 disrupts vaginal epithelial barrier function

Activation of protease‐activated receptor‐2 disrupts vaginal epithelial barrier function

Tryptase compromises corneal epithelial barrier function

Integrin alpha v beta 6 contributes to maintaining corneal epithelial barrier function

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