Ubiquitin in a lower plant

Pollmann, Lutz; Kampen, Jan von; Wettern, Michael

doi:10.1111/j.1432-1033.1991.tb16363.x

Cited by 11 publications

(5 citation statements)

References 43 publications

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“…One of them, The unequivocal identification of CON2 as an ubiquitin-encoding gene was facilitated by the exceptionally high degree of amino acid sequence conservation among ubiquitin proteins. Even the ubiquitin portion of a ubiquitin extension protein from a lower plant (Chlamydomonas reinhardtii) differs only at amino acid position 24 from the higher plant ubiquitins [39]. The deduced amino acid sequence for ubiquitin in parsley was identical with that reported for ubiquitins from other higher plants [40], including Arabidopsis [ 10], sunflower [5] and the monocotyledonous plant maize [ 12].…”

Section: Discussionsupporting

confidence: 54%

Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum

et al. 1993

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Ubiquitin is an omnipresent protein found in all eukaryotes so far analysed. It is involved in several important processes, including protein turnover, chromosome structure and stress response. Parsley (Petroselinum crispum) contains at least two active polyubiquitin (ubi4) genes encoding hexameric precursor proteins. The deduced amino acid sequences of the ubiquitin monomers are identical to one another and to ubiquitin sequences from several other plant species. Analysis of the promoter region of one ubi4 gene revealed putative regulatory elements. In parsley plants, the ubi4 mRNAs were the predominant ubiquitin mRNAs and were present at comparable levels in all plant organs tested. In cultured parsley cells, high levels of ubiquitin gene expression remained unaffected by heat shock, elicitor or light treatment.

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Section: Discussionsupporting

confidence: 54%

Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum

et al. 1993

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“…The transcription of the gene corresponding to the 2.3-kb mRNA was strongly enhanced after heat treatment of C. reinhardtii, as described for polyubiquitin genes from other organisms and makes the corresponding C. reinhardtii gene a member of the family of heat-shock genes in this organism. Since the signal detected at 0.7 kb should represent more than one mRNA, it is not possible to conclude from our data which mRNA contributes to the detected enhancement in the strength of the hybridization signal after heat shock, although former data (Pollmann et al 1991) indicate that it is neither UBI1 nor UBI3 from C. reinhardtii. Western analysis of C. reinhardtii proteins isolated from heat-shocked cells showed an increase in the amount of high-molecular-weight conjugates (Wettern et al 1990) and the appearance of new ubiquitin conjugates (data not shown), but no significant alteration in the amount of free ubiquitin.…”

Section: Discussioncontrasting

confidence: 54%

“…In Northern analysis, however, only two abundant signals were detectable, probably for the following reasons. Pollmann et al (1991) demonstrated that UbCEP52 is encoded by at least two different cDNAs of approximately the same size (702 vs. 732 nucleotides). The similar size of the corresponding mRNAs makes it difficult to separate them elecl~rophoretically, resulting in their appearance as one single intense signal at the appropriate size in Northern analysis.…”

Section: Discussionmentioning

confidence: 98%

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Expression of ubiquitin genes in Chlamydomonas reinhardtii: involvement in stress response and cell cycle

Kampen

Nieländer

Wettern

1995

Planta

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Abstract. Applying different stresses (heat shock, photoinhibition, and chilling) to the unicellular green alga Chlamydomonas reinhardtii led to a characteristic transcription pattern of mRNAs encoding ubiquitin extension proteins [0.7-kb transcript(s)] as well as polyubiquitin (2.3-kb transcript). Heat shock resulted in an increase in the amount of polyubiquitin mRNA (up to tenfold compared with control cells). Chilling in the light led to a sevenfold increase in the amount of 2.3-kb transcript and to a twofold increase in the amount of 0.7-kb mRNA(s), whereas a less pronounced effect on the level of the polyubiquitin transcript was observed after applying either chilling in darkness or photoinhibition. The latter stress, however, led to a dramatic decrease in mRNAs encoding ubiquitin extension proteins (down to 23% of control cells). Experiments performed with a temperature-sensitive cell-cycle mutant of C. reinhardtii showed that the 2.3-kb polyubiquitin mRNA was no longer transcribed when cells were shifted to a non-permissive temperature, and thus were blocked in their vegetative cell cycle. This leads to the assumption that the expression of the corresponding gene is necessary for completion of the C. reinhar&ii vegetative cell cycle.

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“…This has already been shown in the case of yeast [47], barley [24], D. discoideum [58] and A. thaliana [9]. In one case, C. reinhardii, the steady-state level of ubiquitin-CEP mRNAs was found to decrease after heat shock [48]. It was also shown to increase in potato tubers after wounding [50].…”

Section: Discussionmentioning

confidence: 72%

Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses

et al. 1992

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Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5-mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.

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Ubiquitin in a lower plant

Cited by 11 publications

References 43 publications

Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum

Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum

Expression of ubiquitin genes in Chlamydomonas reinhardtii: involvement in stress response and cell cycle

Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses

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